Sequencing Library TaqMan Kit for SOLiD Next-Generation Sequencing

Quantitate Library Input for Use on the SOLiD System

The SOLiD Library TaqMan Quantitation Kit facilitates the quantitation of amplifiable template in a SOLiD library for use in emulsion PCR (ePCR) on the SOLiD System. Complete with TaqMan qPCR mix custom-designed for SOLiD library adaptors and an adapted SOLiD library control*, the kit is a highly specific solution for determining the optimal amount of starting material needed to most effectively carry out ePCR. 

* The SOLiD library control included in the kit is recommended for use only as a qPCR control, and not as a sequence control.

The SOLiD Library TaqMan Quantitation Kit is designed to quantitate libraries accurately regardless of size and can be used on any real-time instrument, using either fast or standard cycling programs. The kit has been validated for the quantitation of SOLiD fragment libraries, SOLiD short and long mate-paired libraries, SOLiD whole transcriptome libraries, SOLiD small RNA libraries,  SOLiD Chip-seq libraries, and SOLiD SAGE™ libraries.

• The TaqMan Assay provides more specificity and accuracy in detecting properly adapted, amplifiable templates over non–probe-based quantitation methods.
• The validated, ready-to-use qPCR standard requires only a simple serial dilution for use in qPCR. It can also be used to determine the optimal concentration of template to use in ePCR.
• The highly robust qPCR mix can accommodate a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantitation range (Figure 1).
• qPCR-based methods use less material for quantitation.