Search Thermo Fisher Scientific
Overview
Size exclusion chromatography (SEC) columns are the ideal choice for monitoring aggregates.
Protein aggregation is a critical quality attribute (CQA) for monoclonal antibodies (mAbs) as well as adeno-associated viruses (AAVs). It plays a significant role in determining the overall quality of these biologics. To detect and quantify aggregates, size-exclusion chromatography (SEC) is the preferred method.
SEC operates on the steric-exclusion principle, where larger species are effectively excluded from the pores of the packing material and elute faster than smaller species. This principle allows for accurate determination of aggregate levels in a sample.
Recommended SEC HPLC columns for monitoring new modality aggregates by their size
HPLC column selection guide
3 ways to search
|
|
|
Resources
Analysis of mAbs and Their Fragments by SEC and Orbitrap Mass Spectrometry
Importance of a Correct UHPLC Instrument Setup for Protein Aggregate Analysis by SEC
Lifetime Stability of SEC Columns for Protein Aggregate Analysis
A Universal Chromatography Method for Aggregate Analysis of mAbs
See Your Protein Therapeutics in High Resolution
Bioinert, reproducible, size exclusion chromatography: SurePac Bio 500 SEC MDi columns and guards
Related resources
Literature
Brochures
Contact us
* Required field
Style Sheet for Komodo Tabs
Style Sheet for Global Design System
Style Sheet for Global Design System
Style Sheet for Komodo Tabs
CMD SchemaApp code