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Make your low-abundance targets visible, spend less time optimizing, and make every one of your precious samples count. Invitrogen Alexa Fluor Plus conjugate technology provides higher signal to noise ratios with minimized cross-reactivity for fluorescent imaging and western blotting.
"That's amazing!". We showed real scientists the incredible images being created using new Invitrogen Alexa Fluor Plus secondary antibodies. Watch their reactions.
Alexa Fluor Plus 405 conjugates allow for improved signal to noise when compared to traditional Alexa Fluor 405 conjugates. Traditional Alexa Fluor 405 secondary antibody (left) compared to Alexa Fluor Plus 405 secondary antibody (right, goat anti-rabbit IgG secondary antibody, (Cat No. A48254) in immunofluorescent analysis of β-III tubulin in fixed HeLa cells. Nuclei were stained with SYTOX Deep Red stain (Cat. No S11380). The images contain overlay of tubulin (cyan), and nuclei (magenta). Images were taken on an EVOS M7000 Imaging system (Cat. No. AMF7000) at 20X magnification.
Alexa Fluor Plus 488 allows you to see greater detail compared to traditional Alexa Fluor 488 conjugates. Traditional Alexa Fluor 488 secondary antibody (left) compared to Alexa Fluor Plus 488 secondary antibody (right, goat anti-mouse IgG secondary antibody, Cat. No. A32723) in immunofluorescent analysis of β-III tubulin in E18 Sprague Dawley primary cortical neuronal cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of neuronal tubulin (green) and nuclei (blue). Images were taken on a confocal microscope at 40x magnification.
Product list: Alexa Fluor Plus 488 Secondary Antibodies* | |
Goat anti-rabbit | A32731 |
Goat anti-mouse | A32723 |
Goat anti-chicken | A32931 |
Goat anti-rat | A48262 |
Goat anti-human | A48276 |
F(ab’)2-goat anti-rabbit | A48282 |
F(ab’)2-goat anti-mouse | A48286 |
Donkey anti-goat | A32814 |
Donkey anti-rabbit | A32790 |
Donkey anti-mouse | A32766 |
Donkey anti-rat | A48269 |
*All Alexa Fluor Plus 488 conjugates available in small pack sizes
Alexa Fluor Plus 555 allows you to see greater detail compared to traditional Alexa Fluor 555 conjugates. Traditional Alexa Fluor 555 secondary antibody (left) compared to Alexa Fluor Plus 555 secondary antibody (right, goat anti-mouse IgG secondary antibody, Cat. No. A32727) in immunofluorescent analysis of β-III tubulin in E18 Sprague Dawley primary cortical neuronal cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of neuronal tubulin (orange) and nuclei (blue). Images were taken on a confocal microscope at 40x magnification.
Product list: Alexa Fluor Plus 555 Secondary Antibodies | |
Goat anti-rabbit | A32732 |
Goat anti-mouse | A32727 |
Goat anti-chicken | A32932 |
Goat anti-rat | A48263 |
Goat anti-human | A48277 |
F(ab’)2-goat anti-rabbit | A48283 |
F(ab’)2-goat anti-mouse | A48287 |
Donkey anti-goat | A32816 |
Donkey anti-rabbit | A32794 |
Donkey anti-mouse | A32773 |
Donkey anti-rat | A48270 |
Alexa Fluor Plus 594 allows you to see greater detail compared to traditional Alexa Fluor 594 conjugates. Traditional Alexa Fluor 594 secondary antibody (left) compared to Alexa Fluor Plus 594 secondary antibody (right, donkey anti-mouse IgG secondary antibody (Cat. No. A32744). Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570) in immunofluorescent analysis of tubulin in U2OS cells. The image contains overlay of tubulin (red) and nuclei (blue). Images were taken on an EVOS FL Auto 2 Imaging System with an Olympus 20X Super Apochromat objective (Cat. No. AMEP4734) at 20X magnification.
Alexa Fluor Plus 647 allows you to see greater detail compared to traditional Alexa Fluor 647 conjugates. Traditional Alexa Fluor 647 secondary antibody (left) compared to Alexa Fluor Plus 647 secondary antibody (right, goat anti-mouse IgG secondary antibody, Cat. No. A32728) in immunofluorescent analysis of β-III tubulin in E18 Sprague Dawley primary cortical neuronal cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of neuronal tubulin (pink) and nuclei (blue). Images were taken on a confocal microscope at 40x magnification.
Product list: Alexa Fluor Plus 647 Secondary Antibodies* | |
Goat anti-rabbit | A32733 |
Goat anti-mouse | A32728 |
Goat anti-chicken | A32933 |
Goat anti-rat | A48265 |
Goat anti-human | A48279 |
F(ab’)2-goat anti-rabbit | A48285 |
F(ab’)2-goat anti-mouse | A48289 |
Donkey anti-goat | A32849 |
Donkey anti-rabbit | A32795 |
Donkey anti-mouse | A32787 |
Donkey anti-rat | A48272 |
*All Alexa Fluor Plus 647 conjugates available in small pack sizes
Alexa Fluor Plus 680 allows you to see greater detail compared to traditional Alexa Fluor 680 conjugates. Traditional Alexa Fluor 680 secondary antibody (left) compared to Alexa Fluor Plus 680 secondary antibody (right, goat anti-chicken IgY secondary antibody, (Cat. No. A32934) in Immunofluorescent analysis of tubulin in A549 cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of tubulin (purple) and nuclei (blue). Images were taken on an EVOS FL Auto 2 Imaging System with an Olympus 20X Super Apochromat objective (Cat. No. AMEP4734) at 20X magnification.
Alexa Fluor Plus 800 shows more bands at lower dilutions, providing greater sensitivity and range of detection compared to traditional 800 near-IR conjugates. Competitor, 800 near-IR secondary antibody (bottom) compared to Alexa Fluor Plus 800 secondary antibody (top, goat anti-rabbit IgG secondary antibody, Cat. No. A32735) in a western blot analysis of heat shock protein 90 (Hsp90) and histone deacetylase 1 (HDAC1).
Alexa Fluor Plus 405 | Alexa Fluor Plus 488* | Alexa Fluor Plus 555 | Alexa Fluor Plus 594 | Alexa Fluor Plus 647* | Alexa Fluor Plus 680 | Alexa Fluor Plus 800 | |
Goat anti-rabbit | A48254 | A32731 | A32732 | A32740 | A32733 | A32734 | A32735 |
Goat anti-mouse | A48255 | A32723 | A32727 | A32742 | A32728 | A32729 | A32730 |
Goat anti-chicken | A48260 | A32931 | A32932 | A32759 | A32933 | A32934 | A32935 |
Goat anti-rat | A48261 | A48262 | A48263 | A48264 | A48265 | — | — |
Goat anti-human | A48275 | A48276 | A48277 | A48278 | A48279 | — | — |
Donkey anti-goat | A48259 | A32814 | A32816 | A32758 | A32849 | A32860 | A32930 |
Donkey anti-rabbit | A48258 | A32790 | A32794 | A32754 | A32795 | A32802 | A32808 |
Donkey anti-mouse | A48257 | A32766 | A32773 | A32744 | A32787 | A32788 | A32789 |
Donkey anti-rat | A48268 | A48269 | A48270 | A48271 | A48272 | — | — |
*All Alexa Fluor Plus 488 and 647 conjugates available in small pack sizes
Our new Alexa Fluor Plus conjugated F(ab’)2 secondary antibodies are cross-adsorbed or pre-adsorbed to increase sensitivity and reduce background staining. F(ab')2 fragment antibodies are divalent antibodies, containing two Fab portions, that are generated by pepsin digestion from a whole IgG antibody in order to remove the Fc region. F(ab’)2 antibodies are approximately 110kDa and because of their smaller size they can more easily penetrate complex tissue models than whole IgG molecules, potentially leading to more antigen binding capability. This property combined with Alexa Fluor Plus conjugation makes these molecules valuable staining tools in immunohistochemistry and immunofluorescent experiments.
Alexa Fluor Plus 488* | Alexa Fluor Plus 555 | Alexa Fluor Plus 594 | Alexa Fluor Plus 647* | |
F(ab’)2-goat anti-rabbit | A48282 | A48283 | A48284 | A48285 |
F(ab’)2-goat anti-mouse | A48286 | A48287 | A48288 | A48289 |
*All Alexa Fluor Plus 488 and 647 F(ab’)2 conjugates available in small pack sizes.
A
B
Comparison of whole IgG with F(ab’)2 Alexa Fluor Plus 555 secondary antibody in human small intestine (Duodenum). (A) Cell junctions were stained using Invitrogen Cadherin-17 Monoclonal Antibody (Cat No. 60351-1-IG) and Invitrogen Alexa Fluor Plus 555 whole IgG secondary antibody. (B) Under matched conditions, cell junctions were stained using the same primary antibody against Cadherin 17 (Cat. No. 60351-1-IG) and Invitrogen F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 (Cat. No. A48287). Nuclei were stained with Hoechst 33342 (Cat. No. H3570). Images were captured on an Invitrogen EVOS M7000 at 4x magnification.
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*All Alexa Fluor Plus 488 and 647 conjugates available in small pack sizes
For Research Use Only. Not for use in diagnostic procedures.