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When it comes to studying gene expression, qPCR and dPCR are complementary technologies that each offer distinct advantages. qPCR is well-suited for accurately quantifying highly expressed genes over a wide range, providing precise measurements, while dPCR shines in detecting and quantifying low-abundance targets with exceptional sensitivity and the ability to provide absolute quantification. Combining these techniques can help researchers gain more comprehensive insights into the analysis of applications such as cancer research.
“Applied Biosystems have been working with us since the very beginning. They have the best system to identify or to validate biomarkers, both in liquid biopsy and tumors. For example, when we were trying to identify biomarkers in liquid biopsies, some markers have very low expression levels. So Applied Biosystems have helped us with new technologies like Absolute Q Digital PCR that allow us to have a better sensitivity to detect these low frequency markers.”
--Olga Vera
Witness the impactful work of Olga Vera, a junior principal investigator at the Health Research Institute of IdiPAZ, as she explores non-genetic alterations in melanoma to help uncover potential biomarkers. Discover how Thermo Fisher Scientific’s advanced qPCR and dPCR technologies, including the QuantStudio Absolute Q Digital PCR System, are supporting her research efforts to detect low-frequency markers and how this research monitoring of blood-based biomarkers may help to improve future patient outcomes.
Learn more about their research in the video below:
For Research Use Only. Not for use in diagnostic procedures.