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With the wide range of Invitrogen Alexa Fluor dyes there is something for every filter and many options for multiplex detection. This series of superior fluorophores:
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Alexa Fluor dye | Absorption max. (nm) | Emission max (nm) | Emission color* | Extinction coefficient** | |
---|---|---|---|---|---|
Alexa Fluor 350 | 346 | 442 | Blue | 19,000 | |
Alexa Fluor 405 | 401 | 421 | Blue | 34,000 | |
Alexa Fluor 430 | 433 | 541 | Green/Yellow | 16,000 | |
Alexa Fluor 488 | 496 | 519 | Green | 71,000 | |
Alexa Fluor 532 | 532 | 553 | Yellow | 81,000 | |
Alexa Fluor 546 | 556 | 573 | Orange | 104,000 | |
Alexa Fluor 555 | 555 | 565 | Orange | 150,000 | |
Alexa Fluor 568 | 578 | 603 | Orange/Red | 91,000 | |
Alexa Fluor 594 | 590 | 617 | Red | 73,000 | |
Alexa Fluor 610 | 612 | 628 | Red | 138,000 | |
Alexa Fluor 633 | 632 | 647 | Far Red | 239,000 | |
Alexa Fluor 635 | 633 | 647 | Far Red | 140,000 | |
Alexa Fluor 647 | 650 | 665 | Near-IR*** | 239,000 | |
Alexa Fluor 660 | 663 | 690 | Near-IR*** | 132,000 | |
Alexa Fluor 680 | 679 | 702 | Near-IR*** | 184,000 | |
Alexa Fluor 700 | 702 | 723 | Near-IR*** | 192,000 | |
Alexa Fluor 750 | 749 | 775 | Near-IR*** | 240,000 | |
Alexa Fluor 790 | 784 | 814 | Near-IR*** | 270,000 | |
* Typical emission color seen through the eyepiece of a conventional fluorescence microscope with appropriate filters. ** Extinction coefficient at lmax in cm–1M–1. *** Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes. |
Source | Principal lines (nm) |
---|---|
Mercury arc lamp | 366, 405, 436, 546, 578* |
Xenon arc lamp | 250-1000* |
Tungsten–halogen lamp | 350-1000* |
Blue diode laser | 405 |
Helium–cadmium laser | 325, 442 |
Argon ion laser | 457, 488, 514 |
Nd:YAG laser | 532** |
Helium–neon laser | 543, 594, 633 |
Yellow diode laser | 561 |
Krypton ion laser | 568, 647 |
Red diode laser | 635 |
* Continuous white-light source. ** Frequency-doubled principal line output. |
If you are using | Try this Alexa Fluor dye |
---|---|
AMCA, coumarin | Alexa Fluor 350 |
Cy2, FITC (fluorescein) | Alexa Fluor 488 |
Cy3, TRITC (rhodamine) | Alexa Fluor 555 |
Rhodamine red | Alexa Fluor 568 |
Texas Red | Alexa Fluor 594 |
Cy5 | Alexa Fluor 647 |
Cy5.5, IR680 | Alexa Fluor 680 |
Cy7 | Alexa Fluor750 |
For Research Use Only. Not for use in diagnostic procedures.