Oncomine BRCA Assay

Try the new Oncomine BRCA Research Assay and save 33% off on your first order*.
Use promotion code RONC33.

The Oncomine BRCA Research Assay was evaluated by 22 laboratories during early-access program. Downoload a poster with data from this evaluation that was presented at the European Congress of Pathology in September 2016.

The Oncomine BRCA Research Assay enables robust performance and reliable, rapid, and consistent high-quality results from every sample.

• Based on proven Ion AmpliSeq technology
• Fully qualified on clinical research samples
• Requires as little as 10 ng DNA input
• Enables 100% exonic coverage, superior uniformity, and high reads (average is >600)
• Detects 5% and below minor-allele frequencies
• Flexible workflow enables full automation
  
  

The Ion Torrent Oncomine BRCA Assay is now available for use on the Ion GeneStudio systems with the Ion Chef Instrument or for use on the Ion Torrent Genexus System.

Figure 1. Ion Torrent Next Generation Sequencing systems

The associated figures demonstrate the superior performance of the Oncomine BRCA Assay. All exons are 100% covered, with an average of 64 bases of flanking sequence into the introns upstream and downstream of each exon, allowing for over 99% confidence of detecting 5% somatic variants. The uniformity and high read  counts ensure high sensitivity and accuracy of both somatic and germline mutation detection, demonstrated with different workflows (and sequencers). The performance has been verified for use on the Ion 530 chips on GeneStudio system and GX5 chip on Genexus system.

Figure 2. 100% exon coverage across both BRCA 1 and BRCA 2 genes, with high uniformity and read counts across all exons, allowing for over 99% confidence of detecting 5% somatic variant.

Figure 3. Superior accuracy in detecting somatic and germline variants that is highly consistent and independent of workflow. At 5% allele frequency, >1,000 SNV and >600 indel variants measured. At 50%, 100% allele frequency, >4,000 SNV and >200 indel variants measured. Positive predictive value (PPV) = true positives/total number of positives. Sensitivity = true positive/(true positives + false positives).

Figure 4. Relative abundance of BRCA exons are plotted. The sample has a deletion in BRCA1 (red) of exons 4–9 (green circle). BRCA2 (blue) has no CNV. The green plot indicates the sample ID amplicons used for normalization.z

For Research Use Only. Not for use in diagnostic procedures.
*Terms and conditions:
This promotion is available only to customers in Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Hungary, Italy, Luxembourg, Netherlands, Norway, Poland, Portugal, Slovakia, Spain, Sweden, Switzerland, South Africa and the United Kingdom on the first purchase of the Oncomine BRCA Research Assay. Offer valid on qualifying orders received no later than December 31, 2016. Discount will apply to the list price in effect at the time the order is received. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.