Chromatography Learning Lab

Advancing the Purification of VSV-G Pseduotyped Lentiviral Vectors by Using Affinity Chromatography

Review the highlights of our discussion on an affinity resin that addresses the major challenges manufacturing processes for cell and gene therapy vectors derived from lentivirus.

Downstream Purification of Adeno-Associated Virus for Large-Scale Manufacturing of Gene Therapies

Learn how affinity chromatography can contribute to the overall viral clearance in the AAV downstream process.

Development of Purification Steps for Several AAV Serotypes Using POROS CaptureSelect AAVX Affinity Chromatography

Learn how a gene therapy company is testing POROS CaptureSelect AAVX affinity resin as a platform solution for the purification of various AAV vectors.

Evolution in AAV Process Development: 2022 and Beyond

Discover what is on the mind of key opinion leaders on how viral vector manufacture is evolving and what we can expect in the future.

Getting a Gene Therapy Product to Market: Pitfalls and How to Prevent Them

Discover key considerations on getting a gene therapy to market from leading experts in the field.

Optimizing AAV Manufacture

Learn more on AAV affinity chromatography and get key considerations when designing a process using affinity capture of AAVs.

Scalable AAV Manufacturing: Addressing Challenges Across the Workflow

Read and get more insight on challenges across the AAV manufacturing workflow and how innovative tools can help improve AAV production.

Current Technological Trends and Advancements in Vector Purification

Read and discover expert opinions in AAV vector purification and share their opinions on future developments in the downstream process of this gene therapy vector.

Platform Optimization for Efficient AAV Purification: Insights from a CDMO

Read and learn how POROS CaptureSelect AAVX resin can be used as a pan-affinity tool for the universal capture of AAV vectors and how a leading CDMO optimized the process of this resin to establish a robust purification platform.

Simplifying AAV Downstream Process and Product Characterization: A Look at Purification and Analytical Tools

Read and discover how to integrate advanced purification and analytical tools into the AAV manufacturing workflow.

Viral Clearance in a Downstream AAV Process: Case Study Using a Model Virus Panel and a Noninfectious Surrogate

Discover how four companies worked together on a study addressing viral clearance in an AAV8 two-step chromatography process.

Optimizing Vector Production and Purification to Enhance Scalable AAV Manufacturing

Gain more insights on how to intensify your AAV purification process through this wash and elution optimization study.

Simplifying Lentiviral Downstream Processing with a Novel Affinity Resin and Robust Analytical Tools

Read and learn more about a new affinity chromatography resin to purify lentiviral particles and rapid analytical tools for titer determination and impurity quantification.

AAV Purification and Scale Up

Explore innovative solutions that surmount technical hurdles and enhance downstream processes, producing high product yield at lower costs.

Viral Vector Purification

Explore recent advancements that can help solve purification challenges for mRNA and viral vectors such as AAV.

Manufacturing and Analytics for Lentivirus and AAV Vectors: A Visual Guide

Review the manufacturing processes and analytical testing for adeno-associated virus (AAV) and lentivirus (LV) side by side.

Advancing the Purification of VSV-G Pseudotyped Lentiviral Vectors by using Affinity Chromatography

Discover a lentiviral purification solution designed to relieve the VSV-G pseudotyped lentivirus particles from suspension cultures.

AAV Purification: Solving Downstream Challenges

Hear from technical specialists in AAV purification and process development who discuss industry trends, platform ability, and the importance of innovation in solving downstream AAV purification challenges.

Enhancing AAV Purification in Gene Therapy Manufacturing

Review the chromatography solutions for the efficient purification of multiple AAV serotypes, including expert insights on bioprocessing and downstream processing optimization

Considerations for Affinity Capture in an AAV Platform Downstream Process

Explore considerations when evaluating a platform affinity capture step for the purification of AAV vectors.

Scalable AAV Manufacturing: Addressing Challenges Across the Workflow

Watch and get more insight on challenges across the AAV manufacturing workflow and how innovative tools can help improve AAV production.

Current Technological Trends and Advancements in Vector Purification

Listen as experts discuss advancements in AAV vector purification and share their opinions on future developments in the downstream process of this gene therapy vector.

Platform Optimization for Efficient AAV Purification: Insights from a CDMO

Watch and learn how POROS CaptureSelect AAVX resin can be used as a pan-affinity tool for the universal capture of AAV vectors and how a leading CDMO optimized the process of this resin to establish a robust purification platform.

Simplifying AAV Downstream Process and Product Characterization: A Look at Purification Analytical Tools

Watch and discover how to integrate advanced purification and analytical tools into the AAV manufacturing workflow.

Viral Safety in AAV Production How Affinity Chromatography Effectively Contributes

Watch and learn how POROS CaptureSelect AAVX chromatography resin can contribute to the overall viral clearance in AAV downstream processing.

Optimizing Vector Production and Purification to Enhance Scalable AAV Manufacturing

Watch this webinar and learn how to intensify your AAV purification process through this wash and elution optimization study.

Simplifying Lentiviral Downstream Processing with a Novel Affinity Resin and Robust Analytical Tools

Watch this webinar and gain insights on a new affinity chromatography resin to purify lentiviral particles and rapid analytical tools for titer determination and impurity quantification.

Driving the Expansion of mRNA into the Therapeutic Sphere

Hear from a panel on the impact of mRNA technology and the innovation that's improving analytical development.

Scalable mRNA Affinity Capture

Expand your understanding of a critical unit operation for both plasmid and mRNA purification, and multiple approaches, each with advantages and pitfalls.

Get in the mRNA Vaccine Race with Affinity Purification

Learn how affinity chromatography provides fast, efficient, and highly scalable methods for mRNA purification.

Supporting Development of mRNA-based Therapies by Addressing Large-Scale Purification Challenges

Read to discover the benefits of a robust, scalable, and easy-to-use purification platform to support production of mRNA therapies.

Optimizing mRNA Purification Conditions by Using a High Throughput Screening Approach

Read and find out how you can optimize your mRNA purification process using high-throughput screening.

COVID-19 mRNA Vaccine Approvals: Key Lessons for Cell & Gene Therapy and mRNA Therapeutic Development

Read the conversation between thought-leaders from both the mRNA vaccine and therapeutics spheres as they share a unique cross-pollination of manufacturing experiences and ideas between both worlds.

Expansion of the RNA Technology Universe

Learn about the emerging technologies for the development of RNA modalities beyond mRNA-based therapeutics.

Optimizing mRNA Purification

Review the advances in mRNA research since its discovery and examine its therapeutic potential in the evolution of mRNA-based modalities.

GEN Spotlight: mRNA Ready to Make Its Mark

Gain knowledge on new development and manufacturing technologies in the mRNA therapy space.

The mRNA Therapeutics Boom

Learn how to simplify workflows and maximize efficiency of mRNA purification with POROS Oligo (dT)25 affinity resin.

mRNA Manufacturing and Analytics

Follow the steps involved and consider critical factors at each stage of the mRNA synthesis process, including downstream purification, formulation, and fill-finish.

Accelerating mRNA-Based Therapy Development with Scalable Purification of In Vitro Transcribed mRNA

Review applications data demonstrating how POROS Oligo (dT)25 helps to address the challenges associated with mRNA purification.

mRNA Platforms and Purification: Challenges and Solutions

Explore the challenges and importance of successful downstream purification of mRNA, as well as the potential of mRNA therapeutics and vaccines for precision therapy.

Driving the Expansion of mRNA into the Therapeutic Sphere

Dive into the expansion of mRNA technology, including the utility of new and emerging downstream purification and analytical solutions.

COVID-19 mRNA Vaccine Approvals: Key Lessons for Cell & Gene Therapy and mRNA Therapeutic Development

Listen to thought-leaders from both the mRNA vaccine and therapeutics spheres share a unique cross-pollination of manufacturing experiences and ideas between both worlds.

Supporting Development of mRNA-based Therapies by Addressing Large-Scale Purification Challenges

Learn the benefits of a robust, scalable, and easy-to-use purification platform to support production of mRNA therapies.

Accelerating mRNA-Based Therapy Development with Scalable Purification of In Vitro Transcribed mRNA

Understand how affinity chromatography can help to simplify workflows and maximize efficiency of your mRNA purification process.

Enabling mRNA-based therapeutics Development Through Efficient and Scalable mRNA Purification Methods

Gain more insight into implementing affinity chromatography in your mRNA purification process.

Supporting Development of mRNA Therapeutics by Enabling Commercial-Scale mRNA Purification

Learn the advantages of affinity capture during mRNA downstream processing at scale.

Optimizing mRNA Purification Conditions by Using a High Throughput Screening Approach

Learn how to optimize your mRNA purification process using high-throughput screening.

Meeting the Global Demand for Non-Monoclonal Antibody Biosimilars

Gain insights on how new approaches to the manufacture of biosimilars have the potential to support sustainable access to critical non-monoclonal antibody biosimilars.

Novel Hydrophobic Interaction Chromatography Next Generation Therapeutic Challenges

Learn how novel HIC resins can provide a solution in the downstream process of a variety of biotherapeutics.

Advantages of Antibody-Based Selectivity in the Purification of Next Generation Biologics

Read to examine the benefits of affinity chromatography for the purification of novel biologics through several case studies.

C-tag Affinity Tag, from Routine Protein Purification to Use in a cGMP Production Process

Review data demonstrating how C-tag, a 4 amino acid affinity tag, can be used for protein detection and purification.

Innovative Purification Solutions for Non-Monoclonal Biosimilars—Opportunities for Process Improvement

Understand how the development of a unique affinity resin enabled a customer to achieve 30% cost savings in their TSH purification process.

Improving Purification Efficiency in the Development of Next-Generation Biologics

Watch to examine the benefits of affinity chromatography for the purification of novel biologics through several case studies.

Affinity Tags Head Toward the Vaccination Clinic (C-Tag) Purification

Learn how the Jenner Institute uses affinity tags to streamline malaria vaccine development.

Get in the mRNA Vaccine Race with Affinity Purification

Learn how a novel affinity chromatography resin contributes to fast, efficient, and scalable mRNA purification.

COVID-19 mRNA Vaccine Approvals: Key Lessons for Cell & Gene Therapy and mRNA Therapeutic Development

Read the conversation between thought-leaders from both the mRNA vaccine and therapeutics spheres as they share a unique cross-pollination of manufacturing experiences and ideas between both worlds.

Addressing Challenges Across the Virus Like Particle Workflow

Read to understand how an integrated system of tools for protein expression, affinity purification, and quality control testing can help support efficient and cost-effective virus like particle production on a commercial scale.

How Affinity Tags Are Speeding Up Malaria Vaccine Development

Read to learn how the C-tag affinity tag system is applied as a standard purification process for candidate malaria vaccines to support efficient translation into clinical trials.

Purification and Quality Control Solutions to Accelerate Vaccine Development

Read to discover how novel chromatography solutions and analytical testing can help improve the downstream processes of different vaccine modalities such as mRNA, recombinant proteins and Virus Like Particles (VLPs).

Managing Purity in Vaccines: How Advanced Purification Technologies are Speeding the Hunt for New Protections Against Disease

Explore recent developments in the purification of various vaccine modalities such as VLPs, mRNA, and protein subunits, through this collection of articles.

Intensify Vaccine Development with Innovative Purification Solutions

Gain more insights on various vaccine modalities and how they are efficiently purified through this collection of articles, webinars, and infographics.

Enabling Rapid Vaccine Development through Manufacturing Innovation and Process Efficiency

Learn how to implement an efficient downstream purification process and avoid bottlenecks in the manufacturing of vaccines and new modalities.

Streamlining Vaccine Development and Production with Modality-specific Purification Tools for mRNA and Beyond

Expand your knowledge on purification tools used for rapid candidate screening and intensified downstream processing of vaccine modalities.

COVID-19 mRNA Vaccine Approvals: Key Lessons for Cell & Gene Therapy and mRNA Therapeutic Development 

Watch a conversation between thought-leaders from both the mRNA vaccine and therapeutics spheres as they share a unique cross-pollination of manufacturing experiences and ideas between both worlds.

Improving and Intensifying Vaccine Purification by Implementing Affinity Chromatography 

Understand how affinity chromatography can aid vaccine development and manufacturing by improving purification of vaccine modalities such as mRNA and protein subunits.

Virus-Like Particle Production in Insect Cells Using Baculovirus Express System: Addressing Challenges Across the Workflow

Watch to understand how an integrated system of tools for protein expression, affinity purification, and quality control testing can help support efficient and cost-effective virus-like particle production on a commercial scale.

Rapid Vaccine Manufacturing for Clinical Trials—Insights from the Malaria Field

Watch to learn how the C-tag affinity tag system is applied as a standard purification process for candidate malaria vaccines to support efficient translation into clinical trials.

Advancing Vaccine Development with Novel Chromatography Solutions and State of the Art Quality Testing 

Watch to discover how novel chromatography solutions and analytical testing can help improve the downstream processes of different vaccine modalities such as mRNA, recombinant proteins, and Virus Like Particles (VLPs).

Accelerating Vaccine Development and Large-Scale Manufacturing with Advanced Purification Tools

Learn how novel purification solutions can help improve the downstream process of different vaccine modalities such as mRNA, recombinant proteins, and Virus Like Particles (VLPs). 

Understanding Viral Clearance During Anion-Exchange Chromatography

Review a method of purification that considers such factors as load pH, virus concentration, and the physical and chemical properties of three different monoclonal antibodies.

Diversity of Single-Domain Antibodies for Purification of Biotherapeutics and Analytical Assays Purification

Learn more about single domain antibodies and how they are ideal ligands to develop a broad array of analytical assays and techniques.

Choosing a Chromatography Resin for the Downstream Purification of Biotherapeutics

Find out how various resin options in the process development toolbox can help you customize the manufacturing processes for your specific biotherapeutic molecule.

Custom Chromatography Solutions for Complex Biotherapeutics Purification

Discover how custom chromatography resins can help overcome purification challenges associated with novel molecules when off-the-shelf chromatography resins are not available.

Advantages of Antibody-Based Selectivity in the Purification of Next Generation Biologics

Examine the benefits of affinity chromatography for the purification of novel biologics through several case studies.

Advances in Downstream Processing of Complex Antibodies

Gain insight into selecting an affinity chromatography ligand to polish complex monoclonal antibodies including specificity, capacity, stability, and cost.

Antibody Therapeutic Polishing: Utility of Hydrophobic-Interaction Chromatography

Explore how two clinical monoclonal antibodies yield aggregate clearance using hydrophobic interaction chromatography for aggressive purification.

Pure and Simple: Affinity Chromatography Solutions for Complex Biotherapeutic Manufacturing

Discover how using affinity purification can help improve biotherapeutic manufacturing of antibody-based therapeutics, mRNA and vaccines, viral vectors, and other novel therapeutics.

Improve Purification Process Efficiency

Learn from experts as they share their views on enhancing downstream processes for various biomolecules using affinity chromatography.

Affinity Resin Binding Solutions

Discover how our purification solutions can deliver efficient and scalable yields for downstream processing.

Pilot-Scale Column Packing with Thermo Scientific POROS 50 µm Resins

Learn how to pack a pilot-scale column with Thermo Scientific POROS chromatography resins.

Lab-Scale Column Packing with Thermo Scientific POROS 50 µm Resins

Learn how to pack a lab-scale column with Thermo Scientific POROS chromatography resins.

DOE Methodology for Downstream Purification of pDNA with Ion-Exchange Resins

Learn about a design of experiment (DoE) study to evaluate and optimize pDNA capture for various downstream purification processes.

Column Packing Talk with Ana LeBris

Review best practices for packing POROS chromatography resins.

Platform Purification for the Production of Complex Biologics

Discover robust chromatography tools that will clear more impurities in a single step while maintaining high recoveries and process flexibilities for complex biomolecules.

Addressing Purification Challenges for Complex Therapeutics with Custom Chromatography Resins

Learn how our custom resin platform and novel affinity ligand technology enables the development of innovative purification resins, providing a solution for challenging downstream processes.

Understanding Viral Clearance During Anion Exchange Chromatography by Using a Novel Design of Experiment Approach

Understand the outcome of a study on viral clearance study during AEX chromatography to improve effectivity and rigor of viral clearance at this step.