Isolation of DNA Fragments and PCR Clean-Up | Thermo Fisher Scientific

Kits for DNA gel extraction, cell-free DNA isolation, PCR clean-up, and microbiome DNA extraction

Whether you prefer organic reagents, filter columns, or magnetic beads, our products for Isolation of DNA fragments are designed for sensitive, scalable extraction and are compatible with a range of downstream applications.

Looking to isolate genomic DNA? Check out genomic DNA extraction by sample type

PCR purification kits

PCR clean-up is a routine but critical laboratory procedure. You need a PCR clean-up method that efficiently removes short primers, unincorporated dNTPs, enzymes, short-failed PCR products, and salts from PCR reactions.

Invitrogen DNA purification kits provide easy, fast and safe methods for isolation of DNA fragments that yield exceptional results. Purified DNA fragments are ready for sequencing, additional PCR, transcription, mapping, cloning, and labeling.

Learn more about purifying your PCR product with PureLink PCR cleanup kits
See how the competitors stack up: Compare efficiency of PCR cleanup kits

Which PCR purification kit is right for you?

	Quick and efficient removal of by-products	Concentrates low-yield PCR products	Simple, reliable, rapid method, 96-well format	Rapid, scalable, magnetic bead format	Standalone magnetic bead formulation for flexible, scalable cleanup
Product name	PureLink PCR Purification Kit	PureLink PCR Micro Purification Kit	PureLink 96 PCR Purification Kit	ChargeSwitch PCR Clean-Up Kit	NEW MagMAX Pure Bind Beads
Format	Silica column	Silica column	96-well silica filter plate	Magnetic beads	Magnetic beads
Prep time	15 mins	10 mins	20 mins	10 mins	20 mins
Sample input volume	50–100 µL	50–100 µL	50–100 µL	25–50 µL	Flexible
DNA fragment size	300 bp to 600 bp	100 bp to 12 kb	100 bp to 12 kb	90 bp to 40 kb	>90 bp
DNA binding capacity	Up to 40 µg	Up to 5 µg	Up to 40 µg	~25 µg per mg beads	Varies based on sample to bead ratio
Automation compatibility	No	No	Yes	Yes	Yes
Pack size	50 preps 250 preps	50 preps 250 preps	4 plates	100 preps 960 preps	50 mL 250 mL 500 mL
Cat. No.	K310001 K310002	K310050 K310250	K3100-96A	CS12000 CS12000-10	A58521 A58522 A58523

DNA cleanup for PCR and next- generation sequencing workflows

The new Applied Biosystems MagMAX Pure Bind beads offer a high‑performing, affordable and sustainable magnetic bead solution for DNA cleanup.

Learn more

Perform both PCR purification and gel extraction?

The Invitrogen PureLink Quick Gel Extraction and PCR Purification Combo Kit contains everything you need for both procedures.

Cell-free DNA isolation

Biological fluid samples, such as plasma, serum, and urine, contain cell-free DNA (cfDNA) and viruses. Isolation of cfDNA and viral nucleic acid is challenging because of its low abundance in typical samples.

Large volumes of biological fluids are sometimes required to obtain sufficient amounts of cfDNA for analysis. Invitrogen/Thermo Fisher Scientific offers scalable products capable of handling these large volumes while efficiently recovering cfDNA from a broad range of sample types.

What is cell-free DNA?

Which cell-free DNA isolation kit is right for you?

	Fast isolation of viral nucleic acid	Easy-to-use, high sensitivity	Automated format, capable of processing large volumes of plasma
Product name	PureLink Viral RNA/DNA Mini Kit	PureLink Pro 96 Viral RNA/DNA Purification Kit	MagMAX Cell-Free DNA Isolation Kit
Sample input volume	500 µL	200 µL	0.5–10 mL
Compatible sample types	Plasma, serum, cerebrospinal fluid	Plasma, serum, cerebrospinal fluid	Plasma, serum, urine
Isolation technology	Silica spin column	Silica microplate	Paramagnetic beads
Prep time	15 mins	35 mins	24 samples in <1 hr
High-throughput compatibility	No	Yes	Yes
Downstream applications	Cloning, qPCR, sequencing, genotyping	Cloning, qPCR, sequencing, genotyping	Cloning, qPCR, sequencing, genotyping
Prep size	50 preps	4 x 96 preps	50 preps

Learn more about cell-free DNA isolation using MagMAX beads

Quick and easy DNA gel extraction

Routine isolation of DNA fragments from agarose gel is made easier with DNA gel extraction kits from Thermo Fisher Scientific. High recovery of DNA fragments is critical to the success of the entire workflow.

Invitrogen DNA gel extraction kits are designed to isolate DNA fragments from agarose gel in 15–30 minutes. The silica membrane technology captures DNA fragments without the need for pH adjustment.

With Invitrogen kits, DNA isolated from agarose gel is free of proteins, dye, and agarose; it is ready to use in a variety of applications, including DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling.

Which DNA gel extraction kit is right for you?

	Environmentally friendly	High DNA binding capacity	Yields highly concentrated DNA
Product name	PureLink Quick Gel Extraction Kit	GeneJET Gel Extraction Kit	GeneJET Gel Extraction and DNA Cleanup Micro Kit
DNA binding capacity	15 µg per column	25 µg per column	10 µg per column
Isolation technology & format	Silica spin/vacuum column	Silica spin/vacuum column	Silica spin/vacuum column
Prep time	30 mins	15 mins	15 mins
DNA fragment size range	40 bp to 10 kb	100 bp to 20 kb	25 bp to 20 kb
Elution volume	50 µL	20 to 50 µL	6 to 10 µL
Automation compatibility	No	No	No
Input sample types	Agarose gel slices	Agarose gel slices	Agarose gel slices, PCR and other enzymatic reaction mixtures

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Figure 1. Amplification of DNA isolated using the PureLink Quick Gel Extraction Kit. PCR amplicons varying in size from 100 bp to 5.4 kb were prepared using recombinant Taq DNA Polymerase. A portion of each PCR reaction was run on a 1% UltraPure Agarose gel (data not shown), and amplicon bands were excised and extracted using the PureLink Quick Gel Extraction Kit. Unpurified and gel-extracted PCR products were loaded onto a 1% agarose gel and visualized using SYBR Safe DNA Gel Stain.

See how the competitors stack up: Comparison of DNA gel extraction kits

Green benefits of the PureLink Quick Gel Extraction Kit

Less hazardous reagents
Improved, lightweight, recyclable packaging
Decreased fuel consumption and greenhouse gas emissions for transport
Less waste disposal

Microbiome DNA purification Kit

The Invitrogen PureLink Microbiome DNA Purification Kit enables rapid isolation of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil.

The kit uses proven PureLink spin column technology, combined with efficient triple-lysis approach and fast removal of inhibitors, to provide excellent yields of purified DNA from bacteria or fungi as well as host DNA.

The versatility of this microbiome DNA purification procedure makes it the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.

Application Note: Rapid 2-hour workflow for detection of select bacteria in stool samples
Medical Science Review: The human microbiota: composition, functions, and therapeutic potential

The PureLink Microbiome DNA Purification Kit offers:

Efficient lysis of all microorganisms (including durable species with thick and complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
Streamlined protocols for a variety of biological samples
Recovery of highly pure DNA compatible with PCR, sequencing, and many other types of downstream analysis

One kit to isolate microbial and host DNA from diverse sample types

The PureLink Microbiome DNA Purification Kit is designed to eliminate the need to order “specialized” kits because it has been optimized for use with a wide range of biological samples. This versatile kit enables microbial (and host, where applicable) DNA purification from the following samples:

Swabs (vaginal, buccal, skin, rectal, environmental)
Transport media
Growth media

Stool
Urine
Saliva
Soil

PCR clean-up