Custom and Custom Plus TaqMan™ Copy Number Assays enable the generation of specific, reproducible and easy-to-interpret copy number results. They are suited for validation studies and large sample screens and provide a flexible targeted approach to copy number analysis.

Each custom assay is a mix of forward primer, reverse primer, and FAM™ dye-labeled TaqMan™ MGB™ probe. Designed to run under universal oligo concentration and thermal cycling conditions, these assays are simple to use. Just add TaqMan™ Genotyping Master Mix and your sample to generate sensitive and reproducible data.

Comparison of TaqMan Copy Number Assay Products

There are two custom assay options to meet your research needs (Custom TaqMan® Copy Number Assays and Custom Plus TaqMan® Copy Number Assays). Both allow you to submit a target sequence to our TaqMan® Assay design pipeline, which uses your input sequence to design custom primer and probe sequences. See the key differences between the two options in Table 1:

Feature Custom Plus TaqMan® Copy Number Assays Custom TaqMan® Copy Number Assays Predesigned TaqMan® Copy Number Assays
Designed with copy number-specific algorithm optimized for performance
Available limited to human and mouse assays 
Contains TaqMan® FAM™ dye-labeled MGB™ probes and 2 unlabeled PCR primers
Targets undergo SNP and repetitive sequencing mask 
Genome-specific check 
Reference assay compatibility check✔ (optional)✔ (optional) 
Assay sequences provided  
Assay context sequences and genome location provided 

Easy Ordering

Browse the GeneAssist™ Copy Number Assay Workflow Builder. Navigate to the human or mouse genomic region of interest to select predesigned assays, or select a targe region for Custom Plus Assay design. Alternatively, submit pre-masked sequences for standard Custom Assay design or submit primer/probe pair sequences for assay synthesis.

Order Custom TaqMan Copy Number Assays

Ordering Information

Catalog NumberProduct NameNo. of 10 µL reactions (384-well plate)No. of 20 µL reactions (96-well plate)
4442487Custom Plus TaqMan® Copy Number Assay, SM720360
4442520Custom Plus TaqMan® Copy Number Assay, MED1,500750
4442488Custom Plus TaqMan® Copy Number Assay, LG5,8002,900
4400294Custom TaqMan® Copy Number Assay, SM720360
4400295Custom TaqMan® Copy Number Assay, MED1,500750
4400296Custom TaqMan® Copy Number Assay, LG5,8002,900

References

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  2. H. Mefford et al. 2008. Recurrent Rearrangements of Chromosome 1q21.1 and Variable Pediatric
  3. Phenotypes. N Engl J Med 359. N Engl J Med 10.1056/nejmoa0805384
  4. Holly N. Cukier et al. 2009. Sample degradation leads to false-positive copy number variation calls in multiplex real-time polymerase chain reaction assays. Anal Biochem 386(2):288-90
  5. Leanne M. Dibbens et al. 2009. Familial and sporadic 15q13.3 microdeletions in idiopathic generalized epilepsy: precedent for disorders with complex inheritance. Human Molecular Genetics Vol. 18, No. 19 3626–31 doi:10.1093/hmg/ddp311
  6. Spencer KL et al. 2010. Dissection of Chromosome 16p12 Linkage Peak Suggests a Possible Role for CACNG3 Variants in Age-Related Macular Degeneration Susceptibility. Invest Ophthalmol Vis Sci [Epub ahead of print]
  7. Güney Bademci et al. 2010. A Rare Novel Deletion of the Tyrosine Hydroxylase Gene in Parkinson Disease. Hum Mutat. E1767-71 Online
  8. Louise C. Laurent et al. 2011. Dynamic Changes in the Copy Number of Pluripotency and Cell Proliferation Genes in Human ESCs and iPSCs during Reprogramming and Time in Culture. Cell Stem Cell 8, 106–118
  9. Ping Mayo et al. 2010. CNV Analysis Using TaqMan® Copy Number Assays. Current Protocols in Human Genetics 2.13.1-2.13.10
  10. Anuradha Ramamoorthya et al. 2010. Differential quantification of CYP2D6 gene copy number by four different quantitative real-time PCR assays. Pharmacogenetics and Genomics Vol 20 No 7 452-54
  11. R. Stephanie Huang et al. 2009. Population-specific GSTM1 copy number variation. Human Molecular Genetics Vol. 18, No. 2 366–72
  12. Amanda K. Huber et al. Analysis of Immune Regulatory Genes' Copy Number Variants in Graves' Disease. Thyroid 21(1): 69-74. doi:10.1089/thy.2010.0262
  13. Tram Kim Lam et al. 2009. Copy Number Variants of GSTM1 and GSTT1 in Relation to Lung Cancer Risk in a Prospective Cohort Study. Ann Epidemiol 19(8):546-52. Epub 2009 Apr 25
  14. Emeli Lundström et al. 2011. Effects of GSTM1 in Rheumatoid Arthritis; Results from the Swedish EIRA study. PLoS One 6(3): e17880. doi:10.1371/journal.pone.0017880
  15. Donald F. Conrad et al. 2010. Origins and functional effect of copy number variation in the human genome. Nature 464(7289):704-12. Epub 2009 Oct 7
  16. de Kovel CG et al. 2010. Recurrent microdeletions at 15q11.2 and 16p13.11 predispose to idiopathic generalized epilepsies. Brain 133(Pt 1):23-32. Epub 2009 Oct 20
  17. Tannour-Louet M et al. 2010. Identification of de novo copy number variants associated with human disorders of sexual development. PLoS One 5(10):e15392
  18. Tuch BB et al. Tumor transcriptome sequencing reveals allelic expression imbalances associated with copy number alterations. PLoS One 5(2):e9317
  19. Hakan Thonberg et al. 2011. Mutation screening of patients with Alzheimer disease identifies APP locus duplication in a Swedish patient. BMC Research Notes 4:476
  20. Nori Matsunami et al. 2013. Identification of Rare Recurrent Copy Number Variants in High-Risk Autism Families and Their Prevalence in a Large ASD Population. PLoS One 8(1):e52239
  21. Low copy number of the salivary amylase gene predisposes to obesity
    VOLUME 46. NUMBER 5. MAY 2014 Nature Genetics
    Mario Falchi et. al.