Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
This kit permits quick and easy determination of cell viability using two common microscope filters (FITC and TRITC) based on intracellular esterase activity and plasma membrane integrity.
1. Culture cells in appropriate medium and vessel for microscopy |
2. Thaw vials |
3. Transfer Live Green vial (A) into Dead Red vial (B) |
4. Mix to create 2X stock |
5. Add equal volume 2X stock to cells |
6. Incubate 15 min at 20–25°C |
7. Image cells |
Live Green | Dead Red | |
---|---|---|
Excitation/Emission | 488/515 nm | 570/602 nm |
Standard filter set | FITC or GFP | Texas Red |
EVOS Light Cube | GFP | Texas Red |
Storage conditions | –20°C | –20°C |
Hep G2 cells stained using the LIVE/DEAD Cell Imaging Kit.