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Thermo Scientific Molecular Cloning
Molecular cloning, a key component of the molecular biology workflow, is used to assemble recombinant DNA molecules and to direct their replication. In the molecular cloning workflow, the DNA to be cloned is identified and treated with enzymes to generate DNA fragments. These fragments are then combined with vector DNA to generate recombinant DNA molecules.
Learn more about the high-quality PCR cloning kits, bacterial expression and ligase-independent cloning kits, enzymes, competent cells, and DNA vectors you need for efficient molecular cloning.
PCR cloning workflow reagents
An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Ideal for both blunt-end and sticky-end cloning.
Thermo Scientific DH10B, DH5α, and BL21(DE3) competent cells are produced with stringent quality control to enable reliable, consistent performance in cloning workflows. Thermo Scientific DH10B competent cells are also included in the Thermo Scientific CloneJET PCR Cloning Kit and in the Thermo Scientific Phusion Site-Directed Mutagenesis Kit.
Ligation- independent cloning (LIC)
Designed for fast and efficient LIC and tight regulation of gene expression in E. coli. LIC enables high-cloning efficiencies of more than 95% and eliminates the need for restriction enzyme digestion and ligation steps.
DNA ligation and end repair
Allows fast sticky-end or blunt-end DNA ligation, typically in only 5 minutes at room temperature.
Used for blunting and phosphorylation of DNA ends, typically in just 5 minutes, for subsequent use in blunt-end ligation.
Molecular Cloning Enzymes and Reagents
Thermo Scientific competent cells are produced with stringent quality control to enable reliable, consistent performance in cloning workflows.
Enzymes for modification of DNA ends, such as T4 DNA ligase, alkaline phosphatase, PNK, Klenow fragment and T4 DNA Polymerase.
IPTG and X-gal for blue/white colony selection.
pUC plasmid and lambda DNA for use as molecular cloning vectors.
Thermo Scientific competent cells are produced with stringent quality control to enable reliable, consistent performance in cloning workflows.
Enzymes for modification of DNA ends, such as T4 DNA ligase, alkaline phosphatase, PNK, Klenow fragment and T4 DNA Polymerase.
IPTG and X-gal for blue/white colony selection.
pUC plasmid and lambda DNA for use as molecular cloning vectors.
Other molecular cloning products
Quick and convenient preparation of competent E. coli cells for high transformation efficiencies.
Removal of N-terminal fusion tags from recombinant protein preparations.
Complete your molecular biology workflow
DNA ladders
Wide range of DNA Ladders for accurate DNA sizing and quantification in agarose or polyacrylamide gels.
dNTPs, NTPs and modified nucleotides
High purity nucleotides for DNA polymerization reactions.
cDNA synthesis kits
Reverse transcriptases, primers, nucleotides and and kits for efficient cDNA synthesis.
High-fidelity DNA polymerases and master mixes
Phusion High-Fidelity DNA Polymerases, kits and master mixes.
Video: How to achieve fast and efficient ligation
How to video to demonstrate how to achieve fast and efficient ligation as part of a cloning workflow using the Thermo Scientific Rapid DNA Ligation Kit.
Resources
Find videos, webinars, articles, and tools in our Molecular biology resource library