Native Mass Spectrometry

On-demand Webinar: Automating Native Mass Spectrometry Through Online Buffer Exchange (OBE)

Learn how to optimize protein overexpression with an online buffer exchange (OBE) for nMS applications, how the experiment is implemented, and how it can be extended by coupling to affinity separation (e.g., IMAC-OBE).

View webinar ›

Direct Mass Technology Mode Decipher complexity with clarity

The Thermo Scientific Direct Mass Technology mode redefines mass spectrometry by equipping Thermo Scientific Q Exactive UHMR Hybrid Quadrupole-Orbitrap mass spectrometers with charge detection. Learn how this technology provides direct, accurate mass determination, enabling you to decipher protein complexes, biotherapeutics, and viral particles that are too complex to resolve using ensemble methods.

Learn more › 

Native MS

Publications

• Production and characterization of an AAV1-VP3-only capsid: An analytical benchmark standard
• Online Buffer Exchange Enables Automated Membrane Protein Analysis by Native Mass Spectrometry
• Three-dimensional structure determination of protein complexes using matrix-landing mass spectrometry
• Native Mass Spectrometry-Based Screening for Optimal Sample Preparation in Single-Particle Cryo-EM
• Rapid Online Buffer Exchange for Screening of Proteins, Protein Complexes, and Cell Lysates by Native Mass Spectrometry
• Analysis of Tagged Proteins Using Tandem Affinity-Buffer Exchange Chromatography Online with Native Mass Spectrometry
• Adeno-associated virus capsid assembly is divergent and stochastic
• Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer
• Native Top-Down Mass Spectrometry with Collisionally Activated Dissociation Yields Higher-Order Structure Information for Protein Complexes
• Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins

Posters

• Establishing a Decision Tree for Native Mass Spectrometry Analysis of Membrane Proteins in Complex Membrane Mimetics
• Automated High-throughput Online Native MS Screening for Proteins and Protein Complexes
• Increased sensitivity and throughput for native intact mass analysis using an online buffer exchange column
• Glycoprotein characterization combining native Mass Spectrometry and Direct Mass Technology

Application notes and technical notes

• Rapid online buffer exchange
• Rapid Online Buffer Exchange for Protein Screening

Webinars

• Direct Analysis of Glycosylated Therapeutics
  Wendy Sandoval, Genentech
• Automating Native Mass Spectrometry Through the Use of Online Buffer Exchange
  In this webinar, Vicki Wysocki from Ohio State University will describe the integration of a new online buffer exchange (OBE) column for native MS (nMS) applications. The workflow will simplify and save time in nMS analysis. She will present data on how this workflow is performed and further extended to affinity separation to study protein overexpression.

Q&A Document

• Q&A document from the Vicki Wysocki Webinar
  Numerous questions were asked during the webinar that we have captured in this Q&A document

Direct Mass Technology mode

Publications

• Production and characterization of an AAV1-VP3-only capsid: An analytical benchmark standard
• Mapping the architecture of the initiating phosphoglycosyl transferase from S. enterica O-antigen biosynthesis in a liponanoparticle
• Resolving heterogeneous macromolecular assemblies by Orbitrap-based single-particle charge detection mass spectrometry
• Frequency chasing of individual megadalton ions in an Orbitrap analyser improves precision of analysis in single-molecule mass spectrometry
• Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes
• Isotopic Resolution of Protein Complexes up to 466 kDa Using Individual Ion Mass Spectrometry
• Exploring Charge-Detection Mass Spectrometry on Chromatographic Time Scales
• Combining Surface-Induced Dissociation and Charge Detection Mass Spectrometry to Reveal the Native Topology of Heterogeneous Protein Complexes
• Automated Control of Injection Times for Unattended Acquisition of Multiplexed Individual Ion Mass Spectra
• STORI Plots Enable Accurate Tracking of Individual Ion Signals
• Individual Ion Mass Spectrometry Enhances the Sensitivity and Sequence Coverage of Top Down Mass Spectrometry

Posters

• Glycoprotein characterization combining native Mass Spectrometry and Direct Mass Technology

Webinars

• Characterization of Biotherapeutic Beasts by Mass Spectrometry
  Thierry Bessen, Novartis