CellSensor® Vectors

Analyze key pathways using CellSensor® Vectors

CellSensor® Vectors combine the power of GeneBLAzer® Technology with lentiviral expression to enable efficient signal transduction pathway analysis in any cell line. As an alternative to viral transduction, you can introduce these vectors into your cell using standard transfection techniques with Lipofectamine™ 2000 reagent.

Use these vectors to interrogate key pathways activated or down-regulated by compounds and to determine compound potency and selectivity in your cell line of choice. CellSensor® Vectors:

• Enable ratiometric analysis of disease relevant signal transduction pathways in your cell line
• Permit visualization of activity and use of flow cytometry to rapidly establish optimized stable cell lines
• Provide optimal reporter response and high Z’-factors as a result of functional testing and sequence validation

CellSensor® Vectors are available with a wide range or response elements for analysis in your cell line of choice, with additions to the offering being made regularly. In addition, the pLenti-bsd/MCS-bla vector can be used to create the reporter construct of your choice. pLenti destination vectors, which contain neomycin, Zeocin™, or Blasticidin antibiotic resistance genes for stable selection, are compatible with Gateway(T) technology and are useful for high-level expression of a target gene of interest in mammalian cell lines. All CellSensor® Vectors provide superior response profiles as a result of response element optimization and sequence verification.

Cell Sorting workflow for selection of functional clones using CellSensor® Vectors

CellSensor® Vectors use GeneBLAzer® Beta-lactamase Technology to rapidly establish stably expressing functional clones using flow cytometry techniques. With this approach, functional clones can be selected quantitatively and rapidly, compared to the “blind” selection of traditional colony picking methods using antibiotic selection markers. A typical cell sorting strategy for efficient selection of optimal clones is outlined below.