Affinity Chromatography Resins—Antibody Therapeutics

artistic 3D model of a monoclonal antibody

We have developed an innovative portfolio of affinity chromatography resins to help you solve challenges in the downstream process of next-generation antibody therapeutics. These resins are specifically designed for the purification of engineered monoclonal antibody (mAbs) modalities.

Antibody purification by affinity chromatography

CaptureSelect affinity chromatography resins, specifically developed to bind antibody-subdomain regions are a key solution for the purification of novel antibody therapeutics, such as bi-specific monoclonal antibodies, Fab fragments, and Fc-fusion proteins. These resins are based on the CaptureSelect technology, enabling increased purity and yield in a single purification step.

Selectivity of the antibody affinity resins

Due to the unique selectivity of each resin, a broad range of antibody modalities can be captured. The antibody-subdomain-specific resins help to improve downstream processing of monoclonal antibodies and antibody fragments, and serve as an alternative solution when Protein A does not yield optimal results.

CaptureSelect affinity resins antibody subdomain diagram

CaptureSelect affinity resins are scalable platform purification solutions, non-animal derived, and suitable for cGMP manufacturing.

Benefit from:

High capacity, addressing process demand and high-titer feedstocks
Mild elution conditions for successful purification of pH-sensitive modalities
Efficient antibody fragment purification, without co-purification of light chains
Alternative purification solutions when Protein A doesn't yield desired results

IgE purification

In addition to our subdomain-specific IgG resins, we offer a highly specific resin for when you are working on IgE drug development. The CaptureSelect IgE affinity matrix helps provide efficient purification of IgE from plasma and recombinant sources.

Rapid implementation of novel affinity purification

This article describes the performance of CaptureSelect antibody affinity resins, including rapid implementation into a commercial manufacturing process of a next-generation antibody therapeutic.

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Bioprocess Resin Selection Tool

Use this interactive decision making tool to determine the right chromatography resin for your purification process.

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Find your desired antibody affinity matrix

All CaptureSelect antibody purification resins are available as loose resin in different unit sizes. For your process development needs, the resins are available in pre-packed columns in high-throughput screening format and for bench-scale screening purposes. Additionally, when your unique purification needs cannot be met with off-the-shelf resins, our custom chromatography resin service can help with solving your specific challenge. Click on the product table below to see all available resins.

CaptureSelect affinity matrix

Resin	Purifies	Characteristics	Unit size	Catalog number
CaptureSelect CH1-XL	Fab fragments Human IgG	Fab fragment purification platform (all Fab κ/λ) No binding of free light chains Efficient elution at milder pH	5 mL	1943462005
			10 mL	1943462010
			50 mL	1943462050
			250 mL	1943462250
			1 L	194346201L
			5 L	194346205L
CaptureSelect KappaXP	Human Ig (all isotypes) Fab fragments containing a kappa light chain	High dynamic binding capacity Efficient elution at milder pH Suitable for bi-specific antibodies	5 mL	2943212005
			10 mL	2943212010
			50 mL	2943212050
			250 mL	1943212250
			1 L	194321201L
			5 L	194321205L
CaptureSelect LambdaXP	Human Ig (all isotypes) Fab fragments containing a lambda light chain	High dynamic binding capacity Efficient elution at milder pH Suitable for bi-specific antibodies	5 mL	2943752005
			10 mL	2943752010
			50 mL	2943752050
			250 mL	1943752250
			1 L	194375201L
			5 L	194375205L
CaptureSelect FcXP Affinity Matrix	Fc-fusion proteins Poor Protein A-binding IgG molecules	High dynamic binding capacity Efficient elution at milder pH	5 mL	1943712005
			10 mL	1943712010
			50 mL	1943712005
			250 mL	1943712250
			1 L	194371201L
			5 L	194371205L
POROS CaptureSelect FcXP Affinity Resin	Human IgG Fc-fusion proteins	High dynamic binding capacity Efficient elution at milder pH Efficient purification of larger biomolecules	50 mL	A56249
			250 mL	A56251
			1000 mL	A56252
			5000 mL	A56253
			10000 mL	A56254

CaptureSelect Leakage ELISAs

Products incorporating CaptureSelect technology are used for clinical and commercial manufacturing of a variety of biologics. To complement each affinity resin, we offer product specific ELISAs to measure ligand potentially leaching from the column. Click on the product table below to see all available ELISAs.

CaptureSelect leakage ELISAs

Product	Catalog number	Unit size	Complementing resin
CaptureSelect CH1-XL Ligand Leakage ELISA	810346201	1 assay	CaptureSelect CH1-XL
CaptureSelect CH1-XL Ligand Leakage ELISA	810346210	10 assays	CaptureSelect CH1-XL
CaptureSelect KappaXP Ligand Leakage ELISA	810321201	1 assay	CaptureSelect KappaXP
CaptureSelect KappaXP Ligand Leakage ELISA	810321210	10 assays	CaptureSelect KappaXP
CaptureSelect LambdaXP Ligand Leakage ELISA	810375201	1 assay	CaptureSelect LambdaXP
CaptureSelect LambdaXP Ligand Leakage ELISA	810375210	10 assays	CaptureSelect LambdaXP
CaptureSelect FcXP Ligand Leakage ELISA	810371201	1 assay	CaptureSelect FcXP
CaptureSelect FcXP Ligand Leakage ELISA	810371210	10 assays	CaptureSelect FcXP
CaptureSelect IgG-CH1 Ligand Leakage ELISA	810320001	1 assay	CaptureSelect IgG-CH1
	810320010	10 assays
	810320050	50 assays
CaptureSelect KappaXL Ligand Leakage ELISA	810321001	1 assay	CaptureSelect KappaXL
	810321010	10 assays
	810321050	50 assays
CaptureSelect FcXL Ligand Leakage ELISA	810328001	1 assay	CaptureSelect FcXL
CaptureSelect FcXL Ligand Leakage ELISA	810328010	10 assays	CaptureSelect FcXL
CaptureSelect KappaSelect Leakage ELISA	810083301	1 assay	KappaSelect affinity resin*
CaptureSelect KappaSelect Leakage ELISA	810083310	10 assays	KappaSelect affinity resin*
CaptureSelect LambdaFabSelect Leakage ELISA	810308001	1 assay	LambdaFabSelect affinity resin*
CaptureSelect LambdaFabSelect Leakage ELISA	810308010	10 assays	LambdaFabSelect affinity resin*
CaptureSelect IgSelect Leakage ELISA	810083501	1 assay	IgSelect affinity resin*