Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
| Highest purity for molecular assays | Affordable & pure, flow-compatible cells | Untouched—most physiological relevant cells | |||
|---|---|---|---|---|---|
| Isolation method | Depletion or positive isolation | Positive isolation | Negative isolation | ||
| Starting samples | Any sample (whole blood, MNC, bone marrow) | Any sample (whole blood, MNC, bone marrow) | MNC* | ||
| # MNC processed (mL whole blood processed) | 1-2 x 109 MNC 200 mL whole blood | 1-2 x 109 MNC 80 mL whole blood | 1 x 109 cells | ||
| Purity | Highest 98–100% | Higher 95–99% | High 90–95% | ||
| Yield | Highest 95–100% | Standard 45–60% | Standard 50–70% | ||
| Viability | >95% | >95% | >95% | ||
| Isolation time | ~30 min | ~60 min | ~50 min | ||
| Isolation temp. | Cold | Cold | Cold | ||
| Applications | Depletion of unwanted monocytes or positive isolation for molecular isolation of proteins & nucleic acids | Cellular assays (cell culture, cell activation/proliferation, cytokine studies) Molecular isolation of proteins & nucleic acids | Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells) | ||
| Bead-free target cells | No | Yes (but antibody present) | Yes | ||
| Compatible with flow cytometry/sorting | No | Yes | Yes | ||
| Shelf life from production date | 3 years | 2 years | 2 years | ||
| Required materials | Magnets | Magnets | Magnets | ||
| Order now | Order now | Order now |