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Primary Antibodies for the Violet Laser
Detect more with a violet laser
Invitrogen eBioscience Super Bright polymer dyes and Brilliant Violet™ dyes are violet laser-excitable fluorophores that expand the capabilities of multi-parametric flow cytometry analysis. This family of bright fluorophores enables the use of more markers off the violet channel and increases the detection of low-abundance antigens due to their super bright signals. This empowers you to detect more in a single sample and answer complex cell biology questions—in less time and with less sample.
The Brilliant Violet™ polymer dyes join the trusted Invitrogen Alexa Fluor dye, Invitrogen eBioscience Super Bright dye, and Invitrogen eFluor dye portfolios in providing fluorochromes compatible with the 405 nm spectral line of the violet laser.
Properties of fluorescent dyes for the violet laser
The table below lists the properties of the most commonly used fluorophores for the violet laser.
| Fluorophore | Fluorophore type | Ex/Em (nm) | Relative intensity* (1 = lowest, 5 = highest) | Suggested bandpass filter (nm) | Uses |
|---|---|---|---|---|---|
| Alexa Fluor 405 | Organic dye | 401/421 | 1 | 450/50 | Use this conjugate for detection of high-abundance targets |
| eFluor 450 | Organic dye | 405/450 | 2 | 450/50 | Comparable to Pacific Blue dye |
| eFluor 506 | Organic dye | 419/506 | 1 | 510/50 | Use in place of AmCyan or Horizon V500 |
| Brilliant Violet™ 421 | Polymer dye | 407/423 | 4 | 450/50 | BV421 has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometers |
| Brilliant Violet™ 480 | Polymer dye | 440/479 | 3 | 525/40 | BV480 has a medium relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometers |
| Brilliant Violet™ 605 | Polymer dye | 407/605 | 4 | 610/20 | BV605 has a medium-high relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometry |
| Brilliant Violet™ 650 | Polymer dye | 407/649 | 2 | 660/20, 630 LP | BV650 has a low-to-medium relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometers |
| Brilliant Violet™ 711 | Polymer dye | 407/713 | 4 | 710/50, 690 LP | BV711 has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometers |
| Brilliant Violet™ 786 | Polymer dye | 407/786 | 3 | 780/60, 750 LP | BV786 has a medium relative brightness and can be used for cell surface, intracellular, and nuclear staining with conventional and spectral flow cytometers |
| Super Bright 436 | Polymer dye | 414/436 | 4 | 450/50 | Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 421 dye |
| Super Bright 600 | Tandem polymer dye | 414/600 | 5 | 610/20 | Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 605 |
| Super Bright 645 | Tandem polymer dye | 414/645 | 5 | 660/20 | Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 650 dye |
| Super Bright 702 | Tandem polymer dye | 414/702 | 5 | 710/50 | Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 711 dye |
| Super Bright 780 | Tandem polymer dye | 414/780 | 4 | 780/60 | Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 786 dye or Brilliant Violet 785 dye |
| Pacific Blue | Organic dye | 410/455 | 3 | 450/50 | Comparable to eFluor 450 |
| Pacific Green | Organic dye | 410/500 | 2 | 522/31 | Use in place of AmCyan or Horizon V500 |
| Pacific Orange | Organic dye | 400 /551 | 1 | 585/40 | Orange-fluorescent dye; conjugates of this dye are fluorescent even at neutral pH |
| Qdot 525 | Nanocrystal | UV—525 | 3 | 530/30 | Additional choice for multiplexed fluorescence detection |
| Qdot 565 | Nanocrystal | UV—565 | 3 | 574/26 | Additional choice for multiplexed fluorescence detection |
| Qdot 605 | Nanocrystal | UV—561/605 | 3 | 605/20 | Additional choice for multiplexed fluorescence detection |
| Qdot 655 | Nanocrystal | UV—633/655 | 3 | 655/20 | Additional choice for multiplexed fluorescence detection |
| Qdot 705 | Nanocrystal | UV—633/705 | 3 | 695/40 | Additional choice for multiplexed fluorescence detection |
| Qdot 800 | Nanocrystal | UV—633/800 | 3 | 780/60 | Additional choice for multiplexed fluorescence detection |
| * The relative intensity of any fluorophore is dependent upon cell type being studied, reagent used, instrument, and instrument configuration. The relative intensities provided are to be used as a guideline; observed intensities may vary. | |||||
This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.
Contact our technical sales specialists for help choosing antibodies, fluorophores, and reagents for success on the flow cytometer you’ll be using.
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Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.