Reagent Selection Guide for the Attune Flow Cytometers

The Invitrogen Attune cytometers can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry, to match the panels you are currently running. Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers.

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Find reagents for flow cytometry Select fluorophore Find antibodies

For a reagent guide for the first-generation Attune flow cytometer, see Resources for the Original Attune Cytometer

Find compatible reagents by excitation source

Each of the tabs below shows the antibody labels and application-based dyes and fluorescent reagents that are compatible with a given laser excitation source. Green laser configuration not available on the Attune CytPix model.

Violet 6 laser (405 nm) reagents

	Blue	Green	Orange	Red	Far Red	Near IR
Emission filter	450/40 nm	525/50 nm	610/20 nm	660/20 nm	710/50 nm	780/60 nm
Channel name	VL1	VL2	VL3	VL4	VL5	VL6
Compatible fluorescent proteins	▪ Azurite ▪ Cerulean ▪ eBFP ▪ eCFP ▪ mTurquoise ▪ Sirius	▪ T-Sapphire ▪ CFP ▪ vGFP
Antibody labels	▪ Super Bright 436 ▪ eFluor 450 ▪ VioBlue ▪ Brilliant Violet 421 ▪ Pacific Blue ▪ BD Horizon V450	▪ eFluor 506 ▪ VioGreen ▪ Brilliant Violet 510 ▪ Pacific Green ▪ BD Horizon V500	▪ Super Bright 600 ▪ Qdot 605 ▪ Pacific Orange ▪ Brilliant Violet 605	▪ Super Bright 645 ▪ Brilliant Violet 650	▪ Super Bright 702 ▪ Brilliant Violet 711 ▪ Qdot 705	▪ Super Bright 786 ▪ Brilliant Violet 786
Viability dyes (compatible with fixation)	▪ LIVE/DEAD Fixable Violet	▪ LIVE/DEAD Fixable Aqua	▪ LIVE/DEAD Fixable Yellow
Viability dyes (non-fixed cells)	▪ DAPI ▪ SYTOX Blue
DNA content/ cell cycle dyes (live cells)	▪ Vybrant DyeCycle Violet
DNA content/ cell cycle dyes (fixed cells)	▪ FxCycle Violet
Apoptosis dyes	▪ Annexin V AF350 ▪ PO-PRO-1	▪ Violet Ratiometric Probe (F2N12S)	▪ Violet Ratiometric Probe (F2N12S)
Cell proliferation	▪ Click-iT Plus Edu Pacific Blue ▪ CellTrace Violet
ROS detection
Phagocytosis

Violet laser (405 nm) reagents

	Blue	Green	Orange	Red
Emission filter	440/50 nm	512/25 nm	603/48 nm	710/50 nm
Channel name	VL1	VL2	VL3	VL4
Compatible fluorescent proteins	▪ Sirius ▪ Azurite ▪ eCFP ▪ eBFP ▪ mTurquoise ▪ Cerulean	▪ vGFP
Antibody labels	▪ Super Bright 436 ▪ Alexa Fluor 405 ▪ Pacific Blue	▪ Pacific Green ▪ Qdot 525	▪ Super Bright 600 ▪ Pacific Orange ▪ Qdot 605	▪ Super Bright 645 ▪ Qdot 705
Viability dyes (compatible with fixation)	▪ LIVE/DEAD Fixable Violet	▪ LIVE/DEAD Fixable Aqua	▪ LIVE/DEAD Fixable Yellow
Viability dyes (non-fixed cells)	▪ DAPI ▪ SYTOX Blue		▪ PI
DNA content/ cell cycle dyes (live cells)	▪ Vybrant DyeCycle Violet
DNA content/ cell cycle dyes (fixed cells)	▪ FxCycle Violet
Apoptosis dyes	▪ Annexin V AF350 ▪ PO-PRO-1	▪ Violet Ratiometric Probe (F2N12S)	▪ Violet Ratiometric Probe (F2N12S)
Cell proliferation	▪ Click-iT Plus Edu Pacific Blue ▪ CellTrace Violet
ROS detection
Phagocytosis

Blue laser (488 nm) reagents

Color	Green	Orange	Orange	Far Red	Near IR
Emission filter	530/30 nm	574/26 nm	590/40 nm	695/40 nm	780/60 nm
Channel	BL1	BL2 (without yellow laser present)	BL2 (with yellow laser present)	BL3	BL4
Compatible fluorescent proteins	▪ eGFP ▪ Emerald ▪ eYFP	▪ eYFP ▪ mCitrine ▪ Venus
Antibody labels	▪ Alexa Fluor 488 ▪ FITC	▪ Alexa Fluor 546 ▪ Qdot 565 ▪ Qdot 585 ▪ PE	▪ PE-Alexa Fluor 610 ▪ PE-Texas Red ▪ Qdot 585 ▪ PE	▪ PE-Alexa Fluor 700 ▪ PE-Cy5.5 ▪ PerCP ▪ PerCP-Cy5.5 ▪ Qdot 705 ▪ Tri-Color	▪ Pe-Cy7 ▪ PE-Alexa Fluor 750 ▪ Qdot 800
Viability dyes (compatible with fixation)	▪ LIVE/DEAD Fixable Green		▪ LIVE/DEAD Fixable Red
Viability dyes (non-fixed cells)	▪ SYTOX Green	▪ PI ▪ SYTOX Orange	▪ 7-AAD ▪ PI ▪ SYTOX Orange ▪ SYTOX AADVanced	▪ 7-AAD ▪ PI ▪ SYTOX AADVanced
DNA content/ cell cycle dyes (live cells)	▪ Vybrant DyeCycle Green	▪ Vybrant DyeCycle Orange			▪ Vybrant DyeCycle Ruby
DNA content/ cell cycle dyes (fixed cells)			▪ FxCycle PI/Rnase
Apoptosis dyes	▪ Annexin V AF488 ▪ MitoProbe JC-1 ▪ CellEvent Caspase-3/7 Green ▪ anti-PARP-FITC ▪ YO-PRO-1 iodide ▪ APO-BrdU TUNEL - Alexa Fluor 488 ▪ MitoProbe Transition Pore ▪ MitoProbe DiOC₂(3) ▪ Annexin V FITC	▪ Annexin V PE ▪ TMRE ▪ TMRM ▪ MitoProbe JC-1	▪ Annexin V PE ▪ TMRE ▪ TMRM
Cell proliferation	▪ Click-iT Plus Edu Alexa Fluor 488 ▪ CellTrace CFSE
ROS detection	CellROX Green
Phagocytosis	▪ pHrodo Green E. coli BioParticles ▪ pHrodo Green S. aureus BioParticles	▪ pHrodo Red E. coli BioParticles ▪ pHrodo Red Phagocytosis

Green laser (532 nm) reagents

Color	Orange	Red	Far Red	Near IR
Emission filter	575/36 nm	620/15 nm	695/40 nm	780/60 nm
Channel	GL2	GL2	GL3	GL4
Compatible fluorescent proteins	▪ tdTomato ▪ YFP	▪ tmCherry
Antibody labels	▪ Alexa Fluor 532 ▪ Alexa Fluor 555 ▪ DsRed ▪ Qdot 565 ▪ Qdot 605 ▪ R-phycoerythrin (R-PE, PE)	▪ Alexa Fluor 568 ▪ Alexa Fluor 594 ▪ Qdot 605 ▪ PE-Texas Red	▪ Qdot 705 ▪ PE-Alexa Fluor 700 ▪ TRI-COLOR (PE-Cy5) ▪ PE-Cy5.5	▪ Qdot 800 ▪ PE-Cy7
Viability dyes (compatible with fixation)		▪ LIVE/DEAD Fixable Red
Viability dyes (non-fixed cells)	▪ SYTOX Orange	▪ SYTOX AADVanced ▪ PI	▪ SYTOX AADVanced
DNA content/ cell cycle dyes (live cells)	▪ Vybrant DyeCycle Orange	▪ Vybrant DyeCycle Orange	▪ Vybrant DyeCycle Ruby
DNA content/ cell cycle dyes (fixed cells)		▪ FxCycle PI/Rnase
Apoptosis dyes	▪ Annexin V, R-PE ▪ Annexin V, Alexa Fluor 555 ▪ MitoTracker Orange CMTMRos	▪ Annexin V, Alexa Fluor 568 ▪ Annexin V, Alexa Fluor 594 ▪ MitoTracker Red MNXRos
Cell proliferation	▪ CellTrace Yellow	▪ Click-iT Plus EDU ▪ Alexa Fluor 594
ROS detection	▪ CellROX Orange
Phagocytosis	▪ pHrodo Red E. coli BioParticles

Yellow laser (561 nm) reagents

Color	Orange	Red	Far red	Near IR
Emission filter	585/16 nm	620/15 nm	695/40 nm	780/60 nm
Channel	YL1	YL2	YL3	YL4
Compatible fluorescent proteins	▪ mOrange ▪ RFP ▪ dTomato	▪ mCherry ▪ DsRed ▪ mKate ▪ mStrawberry
Antibody labels	▪ Alexa Fluor 568 ▪ PE ▪ Qdot 565 ▪ Qdot 585	▪ Alexa Fluor 594 ▪ PE- Alexa Fluor 610 ▪ PE-Texas Red	▪ PE-Alexa Fluor 700 ▪ PE-Cy5.5 ▪ Qdot 705 ▪ TRI-COLOR	▪ PE-Alexa Fluor 750 ▪ PE-Cy7 ▪ Qdot 800
Viability dyes (compatible with fixation)
Viability dyes (non-fixed cells)	▪ PI ▪ SYTOX Orange	▪ 7-AAD ▪ PI ▪ SYTOX AADvanced ▪ SYTOX Orange	▪ 7-AAD ▪ PI ▪ SYTOX AADvanced
DNA content/cell cycle dyes (live cells)	▪ Vybrant DyeCycle Orange			▪ Vybrant DyeCycle Ruby
DNA content/cell cycle dyes (fixed cells)		▪ FxCycle PI/Rnase
Apoptosis dyes	▪ Annexin V AF568 ▪ Annexin V AF594 ▪ MitoTracker Red CMXRos ▪ MitoTracker Orange CMTMRos ▪ TMRM
Cell proliferation
ROS detection	▪ CellROX Orange
Phagocytosis

Red (637 nm) laser reagents

Color	Far Red	Far Red	Near IR
Emission filter	670/14 nm	720/30 nm	780/60 nm
Channel	RL1	RL2	RL3
Compatible fluorescent proteins
Antibody labels	▪ APC ▪ Alexa Fluor 647 ▪ Alexa Fluor 660 ▪ Qdot 655	▪ Alexa Fluor 680 ▪ Alexa Fluor 700 ▪ APC-Alexa Fluor 700 ▪ Qdot 705	▪ APC-Alexa Fluor 750 ▪ APC-Cy7 ▪ Qdot 800
Viability dyes (compatible with fixation)	▪ LIVE/DEAD Fixable Far Red		▪ LIVE/DEAD Fixable Near-IR
Viability dyes (non-fixed cells)	▪ SYTOX Red
DNA content/cell cycle dyes (live cells)			▪ Vybrant DyeCycle Ruby
DNA content/cell cycle dyes (fixed cells)	▪ FxCycle Far Red
Apoptosis dyes	▪ Annexin V APC ▪ Annexin V AF647 ▪ Annexin V AF 680 ▪ TO-PRO-3 ▪ MitoProbe DilC₁(5)
Cell proliferation	▪ Click-iT Plus Edu Alexa Fluor 647 ▪ CellTrace Far Red
ROS detection	▪ CellROX Deep Red
Phagocytosis

Violet 6 (405 nm)

Violet 6 laser (405 nm) reagents

	Blue	Green	Orange	Red	Far Red	Near IR
Emission filter	450/40 nm	525/50 nm	610/20 nm	660/20 nm	710/50 nm	780/60 nm
Channel name	VL1	VL2	VL3	VL4	VL5	VL6
Compatible fluorescent proteins	▪ Azurite ▪ Cerulean ▪ eBFP ▪ eCFP ▪ mTurquoise ▪ Sirius	▪ T-Sapphire ▪ CFP ▪ vGFP
Antibody labels	▪ Super Bright 436 ▪ eFluor 450 ▪ VioBlue ▪ Brilliant Violet 421 ▪ Pacific Blue ▪ BD Horizon V450	▪ eFluor 506 ▪ VioGreen ▪ Brilliant Violet 510 ▪ Pacific Green ▪ BD Horizon V500	▪ Super Bright 600 ▪ Qdot 605 ▪ Pacific Orange ▪ Brilliant Violet 605	▪ Super Bright 645 ▪ Brilliant Violet 650	▪ Super Bright 702 ▪ Brilliant Violet 711 ▪ Qdot 705	▪ Super Bright 786 ▪ Brilliant Violet 786
Viability dyes (compatible with fixation)	▪ LIVE/DEAD Fixable Violet	▪ LIVE/DEAD Fixable Aqua	▪ LIVE/DEAD Fixable Yellow
Viability dyes (non-fixed cells)	▪ DAPI ▪ SYTOX Blue
DNA content/ cell cycle dyes (live cells)	▪ Vybrant DyeCycle Violet
DNA content/ cell cycle dyes (fixed cells)	▪ FxCycle Violet
Apoptosis dyes	▪ Annexin V AF350 ▪ PO-PRO-1	▪ Violet Ratiometric Probe (F2N12S)	▪ Violet Ratiometric Probe (F2N12S)
Cell proliferation	▪ Click-iT Plus Edu Pacific Blue ▪ CellTrace Violet
ROS detection
Phagocytosis

Violet (405 nm)

Violet laser (405 nm) reagents

	Blue	Green	Orange	Red
Emission filter	440/50 nm	512/25 nm	603/48 nm	710/50 nm
Channel name	VL1	VL2	VL3	VL4
Compatible fluorescent proteins	▪ Sirius ▪ Azurite ▪ eCFP ▪ eBFP ▪ mTurquoise ▪ Cerulean	▪ vGFP
Antibody labels	▪ Super Bright 436 ▪ Alexa Fluor 405 ▪ Pacific Blue	▪ Pacific Green ▪ Qdot 525	▪ Super Bright 600 ▪ Pacific Orange ▪ Qdot 605	▪ Super Bright 645 ▪ Qdot 705
Viability dyes (compatible with fixation)	▪ LIVE/DEAD Fixable Violet	▪ LIVE/DEAD Fixable Aqua	▪ LIVE/DEAD Fixable Yellow
Viability dyes (non-fixed cells)	▪ DAPI ▪ SYTOX Blue		▪ PI
DNA content/ cell cycle dyes (live cells)	▪ Vybrant DyeCycle Violet
DNA content/ cell cycle dyes (fixed cells)	▪ FxCycle Violet
Apoptosis dyes	▪ Annexin V AF350 ▪ PO-PRO-1	▪ Violet Ratiometric Probe (F2N12S)	▪ Violet Ratiometric Probe (F2N12S)
Cell proliferation	▪ Click-iT Plus Edu Pacific Blue ▪ CellTrace Violet
ROS detection
Phagocytosis

Blue (488 nm)

Blue laser (488 nm) reagents

Color	Green	Orange	Orange	Far Red	Near IR
Emission filter	530/30 nm	574/26 nm	590/40 nm	695/40 nm	780/60 nm
Channel	BL1	BL2 (without yellow laser present)	BL2 (with yellow laser present)	BL3	BL4
Compatible fluorescent proteins	▪ eGFP ▪ Emerald ▪ eYFP	▪ eYFP ▪ mCitrine ▪ Venus
Antibody labels	▪ Alexa Fluor 488 ▪ FITC	▪ Alexa Fluor 546 ▪ Qdot 565 ▪ Qdot 585 ▪ PE	▪ PE-Alexa Fluor 610 ▪ PE-Texas Red ▪ Qdot 585 ▪ PE	▪ PE-Alexa Fluor 700 ▪ PE-Cy5.5 ▪ PerCP ▪ PerCP-Cy5.5 ▪ Qdot 705 ▪ Tri-Color	▪ Pe-Cy7 ▪ PE-Alexa Fluor 750 ▪ Qdot 800
Viability dyes (compatible with fixation)	▪ LIVE/DEAD Fixable Green		▪ LIVE/DEAD Fixable Red
Viability dyes (non-fixed cells)	▪ SYTOX Green	▪ PI ▪ SYTOX Orange	▪ 7-AAD ▪ PI ▪ SYTOX Orange ▪ SYTOX AADVanced	▪ 7-AAD ▪ PI ▪ SYTOX AADVanced
DNA content/ cell cycle dyes (live cells)	▪ Vybrant DyeCycle Green	▪ Vybrant DyeCycle Orange			▪ Vybrant DyeCycle Ruby
DNA content/ cell cycle dyes (fixed cells)			▪ FxCycle PI/Rnase
Apoptosis dyes	▪ Annexin V AF488 ▪ MitoProbe JC-1 ▪ CellEvent Caspase-3/7 Green ▪ anti-PARP-FITC ▪ YO-PRO-1 iodide ▪ APO-BrdU TUNEL - Alexa Fluor 488 ▪ MitoProbe Transition Pore ▪ MitoProbe DiOC₂(3) ▪ Annexin V FITC	▪ Annexin V PE ▪ TMRE ▪ TMRM ▪ MitoProbe JC-1	▪ Annexin V PE ▪ TMRE ▪ TMRM
Cell proliferation	▪ Click-iT Plus Edu Alexa Fluor 488 ▪ CellTrace CFSE
ROS detection	CellROX Green
Phagocytosis	▪ pHrodo Green E. coli BioParticles ▪ pHrodo Green S. aureus BioParticles	▪ pHrodo Red E. coli BioParticles ▪ pHrodo Red Phagocytosis

Green (532 nm)

Green laser (532 nm) reagents

Color	Orange	Red	Far Red	Near IR
Emission filter	575/36 nm	620/15 nm	695/40 nm	780/60 nm
Channel	GL2	GL2	GL3	GL4
Compatible fluorescent proteins	▪ tdTomato ▪ YFP	▪ tmCherry
Antibody labels	▪ Alexa Fluor 532 ▪ Alexa Fluor 555 ▪ DsRed ▪ Qdot 565 ▪ Qdot 605 ▪ R-phycoerythrin (R-PE, PE)	▪ Alexa Fluor 568 ▪ Alexa Fluor 594 ▪ Qdot 605 ▪ PE-Texas Red	▪ Qdot 705 ▪ PE-Alexa Fluor 700 ▪ TRI-COLOR (PE-Cy5) ▪ PE-Cy5.5	▪ Qdot 800 ▪ PE-Cy7
Viability dyes (compatible with fixation)		▪ LIVE/DEAD Fixable Red
Viability dyes (non-fixed cells)	▪ SYTOX Orange	▪ SYTOX AADVanced ▪ PI	▪ SYTOX AADVanced
DNA content/ cell cycle dyes (live cells)	▪ Vybrant DyeCycle Orange	▪ Vybrant DyeCycle Orange	▪ Vybrant DyeCycle Ruby
DNA content/ cell cycle dyes (fixed cells)		▪ FxCycle PI/Rnase
Apoptosis dyes	▪ Annexin V, R-PE ▪ Annexin V, Alexa Fluor 555 ▪ MitoTracker Orange CMTMRos	▪ Annexin V, Alexa Fluor 568 ▪ Annexin V, Alexa Fluor 594 ▪ MitoTracker Red MNXRos
Cell proliferation	▪ CellTrace Yellow	▪ Click-iT Plus EDU ▪ Alexa Fluor 594
ROS detection	▪ CellROX Orange
Phagocytosis	▪ pHrodo Red E. coli BioParticles

Yellow (561 nm)

Yellow laser (561 nm) reagents

Color	Orange	Red	Far red	Near IR
Emission filter	585/16 nm	620/15 nm	695/40 nm	780/60 nm
Channel	YL1	YL2	YL3	YL4
Compatible fluorescent proteins	▪ mOrange ▪ RFP ▪ dTomato	▪ mCherry ▪ DsRed ▪ mKate ▪ mStrawberry
Antibody labels	▪ Alexa Fluor 568 ▪ PE ▪ Qdot 565 ▪ Qdot 585	▪ Alexa Fluor 594 ▪ PE- Alexa Fluor 610 ▪ PE-Texas Red	▪ PE-Alexa Fluor 700 ▪ PE-Cy5.5 ▪ Qdot 705 ▪ TRI-COLOR	▪ PE-Alexa Fluor 750 ▪ PE-Cy7 ▪ Qdot 800
Viability dyes (compatible with fixation)
Viability dyes (non-fixed cells)	▪ PI ▪ SYTOX Orange	▪ 7-AAD ▪ PI ▪ SYTOX AADvanced ▪ SYTOX Orange	▪ 7-AAD ▪ PI ▪ SYTOX AADvanced
DNA content/cell cycle dyes (live cells)	▪ Vybrant DyeCycle Orange			▪ Vybrant DyeCycle Ruby
DNA content/cell cycle dyes (fixed cells)		▪ FxCycle PI/Rnase
Apoptosis dyes	▪ Annexin V AF568 ▪ Annexin V AF594 ▪ MitoTracker Red CMXRos ▪ MitoTracker Orange CMTMRos ▪ TMRM
Cell proliferation
ROS detection	▪ CellROX Orange
Phagocytosis

Red (637 nm)

Red (637 nm) laser reagents

Color	Far Red	Far Red	Near IR
Emission filter	670/14 nm	720/30 nm	780/60 nm
Channel	RL1	RL2	RL3
Compatible fluorescent proteins
Antibody labels	▪ APC ▪ Alexa Fluor 647 ▪ Alexa Fluor 660 ▪ Qdot 655	▪ Alexa Fluor 680 ▪ Alexa Fluor 700 ▪ APC-Alexa Fluor 700 ▪ Qdot 705	▪ APC-Alexa Fluor 750 ▪ APC-Cy7 ▪ Qdot 800
Viability dyes (compatible with fixation)	▪ LIVE/DEAD Fixable Far Red		▪ LIVE/DEAD Fixable Near-IR
Viability dyes (non-fixed cells)	▪ SYTOX Red
DNA content/cell cycle dyes (live cells)			▪ Vybrant DyeCycle Ruby
DNA content/cell cycle dyes (fixed cells)	▪ FxCycle Far Red
Apoptosis dyes	▪ Annexin V APC ▪ Annexin V AF647 ▪ Annexin V AF 680 ▪ TO-PRO-3 ▪ MitoProbe DilC₁(5)
Cell proliferation	▪ Click-iT Plus Edu Alexa Fluor 647 ▪ CellTrace Far Red
ROS detection	▪ CellROX Deep Red
Phagocytosis

Find compatible reagents according to Attune instrument configuration

The antibody labels and fluorescent proteins that are compatible with each Attune Flow Cytometer laser configuration are shown in the expandable sections below.

Blue configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Blue–488 nm	530/30	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	574/26	BL2	Alexa Fluor 546 PE Qdot 565 Qdot 585	eYFP mCitrine Venus
	695/40	BL3	PE-Alexa Fluor 700 PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705 TRI-COLOR
	780/60	BL4	Pe-Cy7 PE-Alexa Fluor 750 Qdot 800

Blue/green configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Blue–488 nm	525/50	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	590/40	BL2	PE PE-Alexa Fluor 610 PE-Texas Red	EYFP mCitrine Venus
	695/40	BL3	PE-Alexa Fluor 700 PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705 TRI-COLOR
Green–532 nm	575/36	GL1	Alexa Fluor 532 Alexa Fluor 555 DsRed Qdot 565 Qdot 605 R-phycoerythrin (R-PE, PE)	tdTomato YFP
	620/15	GL2	Alexa Fluor 568 Alexa Fluor 594 Qdot 605 PE-Texas Red	tmCherry
	695/40	GL3	Qdot 705 PE-Alexa Fluor 700 TRI-COLOR (PE-Cy5) PE-Cy5.5
	780/60	GL4	Qdot 800 PE-Cy7

Blue/yellow configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Blue–488 nm	530/30	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	590/40	BL2	PE PE-Alexa Fluor 610 PE-Texas Red Qdot 585
	695/40	BL3	PE-Alexa Fluor 700 Tri-Color PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705
Yellow–561 nm	585/16	YL1	PE	mOrange RFP dTomato
	620/15	YL2	PE- Alexa Fluor 610 PE-Texas Red	mCherry DsRed mKate mStrawberry
	695/40	YL3	PE-Alexa Fluor 700 PE-Cy5.5 Qdot 705 Tri-Color
	780/60	YL4	PE-Cy7 Qdot 800

Blue/violet configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Violet–405 nm	440/50	VL1	Super Bright 436 Super Bright 645 Alexa Fluor 405 Pacific Blue	Sirius Azurite eCFP eBFP mTarquoise Cerulean
	512/25	VL2	Pacific Green	vGFP
	603/48	VL3	Pacific Orange Qdot 605
	710/50	VL4	Super Bright 702 Qdot 705
Blue–488 nm	530/30	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	574/26	BL2	Alexa Fluor 546 PE Qdot 565 Qdot 585	eYFP mCitrine Venus
	695/40	BL3	PE-Alexa Fluor 700 Tri-Color PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705
	780/60	BL4	Pe-Cy7 PE-Alexa Fluor 750 Qdot 800

Blue/red configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Blue–488 nm	530/30	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	574/26	BL2	Alexa Fluor 546 PE Qdot 565 Qdot 585	eYFP mCitrine Venus
	695/40	BL3	PE-Alexa Fluor 700 Tri-Color PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705
	780/60	BL4	Pe-Cy7 PE-Alexa Fluor 750 Qdot 800
Red–637 nm	670/14	RL1	APC Alexa Fluor 647 Qdot 655
	720/30	RL2	Alexa Fluor 680 Alexa Fluor 700 APC-Alexa Fluor 700 Qdot 705
	780/60	RL3	APC-Alexa Fluor 750 APC-Cy7 Qdot 800

Blue/green/red configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Blue–488 nm	525/50	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	590/40	BL2	PE PE-Alexa Fluor 610 PE-Texas Red	eYFP mCitrine Venus
	695/40	BL3	PE-Alexa Fluor 700 PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705 TRI-COLOR
Green—532 nm	575/36	GL1	Alexa Fluor 532 Alexa Fluor 555 DsRed Qdot 565 Qdot 605 R-phycoerythrin (R-PE, PE)	tdTomato YFP
	620/15	GL2	Alexa Fluor 568 Alexa Fluor 594 Qdot 605 PE-Texas Red	tmCherry
	695/40	GL3	Qdot 705 PE-Alexa Fluor 700 TRI-COLOR (PE-Cy5) PE-Cy5.5
	780/60	GL4	Qdot 800 PE-Cy7
Red–637 nm	670/14	RL1	Alexa Fluor 647 APC Qdot 655
	720/30	RL2	Alexa Fluor 680 Alexa Fluor 700 APC-Alexa Fluor 700 Qdot 705
	780/60	RL3	APC-Alexa Fluor 750 APC-Cy7

Blue/green/violet configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Blue–488 nm	525/50	BL1	Alexa Fluor 488 FITC	EGFP Emerald EYFP
	590/40	BL2	Alexa Fluor 488 FITC	EYFP mCitrine Venus
	695/40	BL3	PE-Alexa Fluor 700 PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705 TRI-COLOR
Green—532 nm	575/36	GL1	Alexa Fluor 532 Alexa Fluor 555 DsRed Qdot 565 Qdot 605 R-phycoerythrin (R-PE, PE)	tdTomato YFP
	620/15	GL2	Alexa Fluor 568 Alexa Fluor 594 Qdot 605 PE-Texas Red	tmCherry
	695/40	GL3	Qdot 705 PE-Alexa Fluor 700 TRI-COLOR (PE-Cy5) PE-Cy5.5
	780/60	GL4	Qdot 800 PE-Cy7
Violet—405 nm	440/50	VL1	Super Bright 436 Super Bright 645 Alexa Fluor 405 Pacific Blue	Azurite Cerulean EBFP ECFP mTurquoise
	512/25	VL2	Pacific Green	T-sapphire
	603/48	VL3	Pacific Orange Qdot 605
	710/50	VL4	Super Bright 702 Qdot 705

Blue/violet/yellow configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Violet–405 nm	440/50	VL1	Super Bright 436 Super Bright 645 Alexa Fluor 405 Pacific Blue	Sirius Azurite eCFP eBFP mTarquoise Cerulean
	512/25	VL2	Pacific Green	vGFP
	603/48	VL3	Pacific Orange Qdot 605
	710/50	VL4	Super Bright 702 Qdot 705
Blue–488 nm	530/30	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	590/40	BL2	PE PE-Alexa Fluor 610 PE-Texas Red Qdot 585
	695/40	BL3	PE-Alexa Fluor 700 Tri-Color PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705
Yellow–561 nm	585/16	YL1	PE	mOrange RFP dTomato
	620/15	YL2	PE- Alexa Fluor 610 PE-Texas Red	mCherry DsRed mKate mStrawberry
	695/40	YL3	PE-Alexa Fluor 700 PE-Cy5.5 Qdot 705 Tri-Color
	780/60	YL4	PE-Cy7 Qdot 800

Blue/red/violet configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Violet–405 nm	440/50	VL1	Super Bright 436 Super Bright 645 Alexa Fluor 405 Pacific Blue	Sirius Azurite eCFP eBFP mTarquoise Cerulean
	512/25	VL2	Pacific Green	vGFP
	603/48	VL3	Pacific Orange Qdot 605
	710/50	VL4	Super Bright 702 Qdot 705
Blue–488 nm	530/30	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	574/26	BL2	Alexa Fluor 546 PE Qdot 565 Qdot 585	eYFP mCitrine Venus
	695/40	BL3	PE-Alexa Fluor 700 Tri-Color PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705
	780/60	BL4	Pe-Cy7 PE-Alexa Fluor 750 Qdot 800
Red–637 nm	670/14	RL1	APC Alexa Fluor 647 Qdot 655
	720/30	RL2	Alexa Fluor 680 Alexa Fluor 700 APC-Alexa Fluor 700 Qdot 705
	780/60	RL3	APC-Alexa Fluor 750 APC-Cy7 Qdot 800

Blue/red/violet/green configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Violet–405 nm	440/50	VL1	Super Bright 436 Super Bright 645 Alexa Fluor 405 Pacific Blue	Azurite Cerulean EBFP ECFP mTurquoise
	512/25	VL2	Pacific Green	T-Sapphire
	603/48	VL3	Pacific Orange Qdot 605
	710/50	VL4	Super Bright 702 Qdot 705
Blue–488 nm	525/50	BL1	Alexa Fluor 488 FITC	EGFP Emerald EYFP
	590/40	BL2	PE PE-Alexa Fluor 610 PE-Texas Red	EYFP mCitrine Venus
	695/40	BL3	PE-Alexa Fluor 700 PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705
Green—532 nm	575/36	GL1	Alexa Fluor 532 Alexa Fluor 555 DsRed Qdot 565 Qdot 605 R-phycoerythrin (R-PE, PE)	tdTomato YFP
	620/15	GL2	Alexa Fluor 568 Alexa Fluor 594 Qdot 605 PE-Texas Red	tmCherry
	695/40	GL3	Qdot 705 PE-Alexa Fluor 700 TRI-COLOR (PE-Cy5) PE-Cy5.5
	780/60	GL4	Qdot 800 PE-Cy7
Red–637 nm	670/14	RL1	Alexa Fluor 647 APC Qdot 655
	720/30	RL2	Alexa Fluor 680 Alexa Fluor 700 APC-Alexa Fluor 700 Qdot 705
	780/60	RL3	APC-Alexa Fluor 750 APC-Cy7

Blue/red/yellow/violet configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Violet–405 nm	440/50	VL1	Super Bright 436 Super Bright 645 Alexa Fluor 405 Pacific Blue	Sirius Azurite eCFP eBFP mTarquoise Cerulean
	512/25	VL2	Pacific Green	vGFP
	603/48	VL3	Pacific Orange Qdot 605
	710/50	VL4	Super Bright 702 Qdot 705
Blue–488 nm	530/30	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	590/40	BL2	PE PE-Alexa Fluor 610 PE-Texas Red Qdot 585
	695/40	BL3	PE-Alexa Fluor 700 Tri-Color PE-Cy5.5 PerCP PerCP-Cy5.5 Qdot 705
Yellow–561 nm	585/16	YL1	PE	mOrange RFP dTomato
	620/15	YL2	PE- Alexa Fluor 610 PE-Texas Red	mCherry DsRed mKate mStrawberry
	695/40	YL3	PE-Alexa Fluor 700 PE-Cy5.5 Qdot 705 Tri-Color
	780/60	YL4	PE-Cy7 Qdot 800
Red–637 nm	670/14	RL1	APC Alexa Fluor 647 Qdot 655
	720/30	RL2	Alexa Fluor 680 Alexa Fluor 700 APC-Alexa Fluor 700 Qdot 705
	780/60	RL3	APC-Alexa Fluor 750 APC-Cy7 Qdot 800

Blue/violet 6 configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Violet–405 nm	440/50	VL1	Super Bright 645 Super Bright 436 eFluor 450 VioBlue Brilliant Violet 421 Pacific Blue BD Horizon V450	Azurite Cerulean eBFP eCFP mTurquoise Sirius
	512/25	VL2	eFluor 506 VioGreen Brilliant Violet 510 Pacific Green BD Horizon V500	T-Sapphire CFP vGFP
	603/48	VL3	Super Bright 600 Qdot 605 Pacific Orange Brilliant Violet 605
	660/20	VL4	Super Bright 645 Brilliant Violet 650
	710/50	VL5	Super Bright 702 Brilliant Violet 711 Qdot 705
	780/60	VL6	Super Bright 786 Brilliant Violet 786
Blue–488 nm	530/30	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	574/26	BL2	PE Alexa Fluor 546 PE-Alexa Fluor 610 PE-Texas Red PE-eFluor 610	eYFP mCitrine Venus
	695/40	BL3	PE-Alexa Fluor 700 PE-Cy5.5 PerCP-Cy5.5 PerCP-eFluor 710 Qdot 705

Blue/red/violet 6 configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Violet–405 nm	440/50	VL1	Super Bright 645 Super Bright 436 eFluor 450 VioBlue Brilliant Violet 421 Pacific Blue BD Horizon V450	Azurite Cerulean eBFP eCFP mTurquoise Sirius
	512/25	VL2	eFluor 506 VioGreen Brilliant Violet 510 Pacific Green BD Horizon V500	T-Sapphire CFP vGFP
	603/48	VL3	Super Bright 600 Qdot 605 Pacific Orange Brilliant Violet 605
	660/20	VL4	Super Bright 645 Brilliant Violet 650
	710/50	VL5	Super Bright 702 Brilliant Violet 711 Qdot 705
	780/60	VL6	Super Bright 786 Brilliant Violet 786
Blue–488 nm	530/30	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
	574/26	BL2	PE Alexa Fluor 546 PE-Alexa Fluor 610 PE-Texas Red PE-eFluor 610	eYFP mCitrine Venus
	695/40	BL3	PE-Alexa Fluor 700 PE-Cy5.5 PerCP-Cy5.5 PerCP-eFluor 710 Qdot 705
Red–637 nm	670/14	RL1	APC Alexa Fluor 647 Qdot 655 eFluor 660
	720/30	RL2	Alexa Fluor 680 Alexa Fluor 700 APC-Alexa Fluor 700 Qdot 705
	780/60	RL3	APC-Alexa Fluor 750 APC-Cy7 Qdot 800 APC-eFluor 780

Blue/red/yellow/violet 6 configuration

Excitation laser	Emission filter (nm)	Channel	Antibody labels	Compatible fluorescent proteins
Violet–405 nm	440/50	VL1	Super Bright 645 Super Bright 436 eFluor 450 VioBlue Brilliant Violet 421 Pacific Blue BD Horizon V450	Azurite Cerulean eBFP eCFP mTurquoise Sirius
	512/25	VL2	eFluor 506 VioGreen Brilliant Violet 510 Pacific Green BD Horizon V500	T-Sapphire CFP vGFP
	603/48	VL3	Super Bright 600 Qdot 605 Pacific Orange Brilliant Violet 605
	660/20	VL4	Super Bright 645 Brilliant Violet 650
	710/50	VL5	Super Bright 702 Brilliant Violet 711 Qdot 705
	780/60	VL6	Super Bright 786 Brilliant Violet 786
Blue–488 nm	530/30	BL1	Alexa Fluor 488 FITC	eGFP Emerald eYFP
Blue–488 nm	695/40	BL3	PE-Alexa Fluor 700 PE-Cy5.5 PerCP-Cy5.5 PerCP-eFluor 710 Qdot 705
Yellow–561 nm	585/16	YL1	Alexa Fluor 555 Qdot 605 R-phycoerythrin (R-PE, PE)	tdTomato mOrange YFP RFP
	620/15	YL2	Alexa Fluor 568 Alexa Fluor 594 Qdot 605 PE-Texas Red PE-Alexa Fluor 610 PE-eFluor 610	mCherry DsRed mKate mStrawberry
	780/60	YL4	Qdot 800 PE-Cy7 PE-Alexa Fluor 750
Red–637 nm	670/14	RL1	APC Alexa Fluor 647 Qdot 655 eFluor 660
	720/30	RL2	Alexa Fluor 680 Alexa Fluor 700 APC-Alexa Fluor 700 Qdot 705
	780/60	RL3	APC-Alexa Fluor 750 APC-Cy7 Qdot 800 APC-eFluor 780

Flow Cytometry Panel Builder

This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.

Resources

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

For Research Use Only. Not for use in diagnostic procedures.