Bigfoot Spectral Cell Sorter Specifications | Thermo Fisher Scientific

Choose your configuration up to 9 lasers and 60 channels

Configurations offering both spectral analysis and conventional compensation range from 5 to 9 lasers and up to 60 parameters, while configurations offering only traditional compensation come with 4 or 5 lasers and up to 35 parameters. Other than lasers and detectors, all configurations share the same specifications.

Download the Complete Bigfoot Cell Sorter Configuration Table

Configurations

All configurations of the Bigfoot Cell Sorter offer two or more forward and side scatter channels in addition to the fluorescence channels shown in the tables.

Configurations offering both spectral analysis and conventional compensation

Lasers	Number of fluorescence detection channels for included lasers									Scatter detectors	Total detection channels	Cat. No.
Lasers	UV 349 nm	Violet 405 nm	Blue violet 445 nm	Blue 488 nm	Green 532 nm	Yellow green 561 nm	Red 594 nm	Far red 640 nm	Near- IR 785 nm	Scatter detectors	Total detection channels	Cat. No.
5	12	12		7		12		5		5	53	PL00302
6	12	12		7		12		5	3	5	56	PL00301
6	12	12	4	7		12		5		5	57	PL00300
7	12	12	4	7		12		5	3	5	60	PL00299
9	12	12	4	7		12		5	3	5	60	PL00285*

*532 nm and 594 nm lasers are not used for spectral analysis, but all fluorescence detectors are used.

Configurations offering only conventional compensation

Lasers	Number of fluorescence detection channels for included lasers									Scatter detectors	Total detection channels	Cat. No.
Lasers	UV 349 nm	Violet 405 nm	Blue violet 445 nm	Blue 488 nm	Green 532 nm	Yellow green 561 nm	Red 594 nm	Far red 640 nm	Near- IR 785 nm	Scatter detectors	Total detection channels	Cat. No.
4		7		7		7		4		2	27	PL00304
5	7	7		5		7		4		5	35	PL00303
4		7		7		7		4		3	28	PL00370

Specifications

Optics

Excitation lasers	349 nm, 405 nm, 445 nm, 488 nm, 532 nm, 561 nm, 594 nm, 640 nm, 785 nm
Optical power	Free space delivery of 349 nm (100 mW), 405 nm (100 mW), 445nm (200 mW), 488 nm (125 mW), 532 nm (100 mW), 561 nm (100 mW), 594 nm (100 mW), 640 nm (100 mW), 785 nm (100 mW)
Beam alignment	Fixed, 7 spatially separated pinholes
Detection parameters	up to 55 fluorescence + 5 scatter
Scatter parameters	Standard FSC and SSC 488 nm; small particle FSC 405 nm; depolarized FSC and SSC 488nm
Scatter resolution	<0.2 µm scatter resolution from background with small particle detector
Pulse measurement	Simultaneous measured peak, area, and width for sample input and output of every channel
Fluorescence sensitivity	<100 MESF for FITC, PE, APC

Fluidics

Nozzles	70, 100, 120, and 150 µm ceramic nozzle tips with adjustable pressure settings
Sample input tube types	1.5 mL, 5 mL, and 15 mL
Temperature control	4°C-37°C for input and output of all media types
Agitation	Automatic based on input tube type

Sorting

Event rate	>100,000 eps acquisition with all 60 parameters
Sorting speed	>70,000 eps with all parameters enabled*
Sort devices	Up to 6-way sorting; configurable tube holders include 1.5 mL, 5 mL, 15 mL, and 50 mL tubes. Microwell plates with a standard footprint of up to 1,536 wells can be loaded directly for straight-down sorting; 4-way sorting into 96-well plates; 4-way sorting into 384 well plates. Temperature control from 4°C to 37°C for all sorting media.

* When running at 70,000 eps, users may see a decrease in efficiency depending on experimental conditions and sort criteria. Sort speeds should always be optimized for specific experiments.

Biosafety

Compliance testing

The Bigfoot Spectral Cell Sorter biosafety enclosure provides personnel and product protection similar to a Class II BSC. Test procedures and criteria laid out within NSF49 and EN12469 standards can be utilized to demonstrate performance.

Other

Dimensions	99 cm W x 99 cm D x 170 cm H (39 in W x 39 in D x 67 in H)
Fluidics cart	None
Weight	1433 lbs (650kg)
Monitor	32-inch, 4k
Power supply	100-240VAC 50/60Hz
Power consumption	750W max (instrument, PC and monitor)
Relative humidity	20-60% RH

Download Specification Sheet

For Research Use Only. Not for use in diagnostic procedures.