Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
ExpiSf CD Medium is a GMP-manufactured, chemically defined, yeastolate-free, serum-free, protein-free, animal origin-free medium specifically designed for high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 (Spodoptera frugiperda) insect cells.
Delivers high reproducibility and lot-to-lot reliability.
Facilitates high-level expression of baculovirus-infected cultures, achieving protein yields three times higher than traditional insect expression platforms when used with other components of the ExpiSf Expression System.
Optimized to support Sf9 and Sf21 cells—achieves Sf9 densities of 10-14 X 106 cells/ml
Sf9 and Sf21 cells pre-adapted to Sf-900 SFM available providing faster turnaround.
Contains lower hydrolysate concentration compared to other commercially available serum free media
Animal origin-free — facilitates regulatory processes.
Optimized for recombinant gene expression in both baculovirus and stable insect expression systems
Optimized for recombinant gene expression by the baculovirus expression by the baculovirus expression vector system (BEVS) in the BTI-TN-5B1-4 (High Five)Trichoplusia ni cell line.
Product | Optimized for | Protein free | Serum free | Animal origin free | Preadapted cell lines available |
ExpiSf CD Medium | Suspension ExpiSf9, Sf9, Sf21 cells | X | X | X | ExpiSF9 Cells (A35243) |
Sf-900 III SFM | Suspension Sf9, Sf21 cells | X | X | Sf9 Cells (12659-017) Sf21 Cells (12682-019) | |
Sf-900 II SFM* | Suspension Sf9, Sf21 cells (Spodoptera frugiperda), TN368 cells (Trichoplusia ni) | X | X | Sf9 Cells (11496-015) Sf21 Cells (11497-013) | |
Express Five SFM* | Suspension BT1-TN-5B1-4 insect cells | X | X | High Five™ Cells (B855-02) |
All four media used for the growth and maintenance of cells in the baculovirus expression vector systems (BEVS) for adherent or suspension culture. Also for large-scale production of recombinant proteins expressed by BEVS.
*Drug Master File Available.
Note: Cell lines from different sources, and different clones of the same cell line, may have highly specific nutritional requirements and may therefore prefer one medium over another. More than one medium formulation (if available) should be evaluated to determine the best option.
Serum Free Media Adapted Cells
For Research Use Only. Not for use in diagnostic procedures.