High-Fidelity Blunt-End TOPO Cloning

Amplification with a high-fidelity proofreading polymerase, such as Invitrogen™ Platinum™ SuperFi™ DNA Polymerase, leaves PCR products with blunt ends rather than 3′-A overhangs, due to the extensive 3′ to 5′ exonuclease activity. Therefore, the vectors supplied in the Invitrogen™ Zero Blunt™ TOPO™ PCR cloning kits contain blunt ends to offer more effective ligation. Choose from over 30 subcloning, sequencing, or long-fragment kits for 95% cloning efficiency in just 5 minutes.

• Quick—avoid extra ligation steps and laborious searches for appropriate restriction enzymes
• Efficient—the lethal ccdB gene on our Zero Blunt TOPO vectors acts as a positive-selection method so that only clones with an insert will grow as colonies on your plate
• Flexible—available with or without competent cells, in multiple reaction sizes and formats to suit your application