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We offer a broad range of options and configurations for ordering custom DNA oligos. Review the information below to learn more about our minimum yield guarantees, delivery schedules, and resources for ordering.
To learn more about purification and modification options, visit our Oligo Modifications & Configurations page.
Our quality control processes help increase performance, speed, and capacity. The result is quick and efficient delivery. Below, find additional information about estimated yields based on length of oligo and starting synthesis scale, as well as minimum yields based on synthesize scale and purification option chosen.
When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmol amounts post-synthesis.
A universal characteristic of chemical synthesis of oligonucleotides is that the final yield is less than the starting synthesis scale. Despite coupling efficiencies greater than 99%, the iterative addition of each base in the oligo chain compounds the loss of potential full-length material you can achieve. The expected final yield is, therefore, dependent on the length of the oligo. In addition, some material loss also occurs during processing, purification, and quality control. For an example of how coupling efficiency affect final yield, see our FAQ on this topic.
Table 1 shows the estimated nmol yield for desalted oligos at each starting synthesis scale. This table converts the OD units into nmol values for an oligo with an equal and even distribution of each base. The actual nmol conversion from OD for a specific oligo may be different based on the composition and distribution of each base within the oligo.
| Oligo length | 25 nmol (2 OD) | 50 nmol (4 OD) | 200 nmol (16 OD) |
|---|---|---|---|
| 20 | 10 | 20 | 80 |
| 25 | 8 | 16 | 64 |
| 30 | 6.6 | 13.2 | 52.8 |
| 35 | 5.6 | 11.2 | 44.8 |
| 40 | 5 | 10 | 40 |
| 45 | 4.5 | 9 | 36 |
| 50 | 4 | 8 | 32 |
| 60 | 3.2 | 6.4 | 25.6 |
| 70 | 2.8 | 5.6 | 22.4 |
| 80 | 2.4 | 4.8 | 19.2 |
When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. The estimated yields above provide guidance on the amount of full-length material we typically achieve, but sequence, GC content, and other factors can vary the actual final yield. The calculator below provides the minimum yield we will ship for unmodified oligos of a given length, scale, and purification method. For modified oligos, please enter your oligo in the online oligo ordering portal to calculate minimum yields. Note: We guarantee the total yield of oligonucleotide as a minimum number of nmol.
Explore: Oligo minimum yield calculator
For account specific pricing, simply add your oligonucleotide item(s) to your cart. Review the tables below for more details on the pricing for regular and next day service, and special handling features.
For pricing, please contact your local office or distributor. For next-day service, orders can only be placed online.
| Format | Synthesis scale | Purification | Size of order | Order cut-off time | |
|---|---|---|---|---|---|
| Specifications | Tube | 25 nmol | Desalted | 1–20 oligos | 1 p.m. EST |
| Price/base (7-40) | $0.38 (USD) | ||||
| Next-day delivery charges | |
|---|---|
| Next-Day Service fee per order* | $19.95 (USD) |
| *Thermo Fisher Scientific will credit back the service fee if we are unable to ship the full order on time for next business day delivery. Please allow up to one week for the credit to be processed. | |
| For plate orders | Price (USD) |
|---|---|
| Aliquoting, per oligo | $0.26 |
| Provide remainders, per oligo | $0.30 |
| Pooling, per oligo pair to be pooled | $0.25 |
| Volume normalization | Free |
| Concentration normalization | Free |
| Volume and concentration normalization | Free |
Prices are included for your planning purposes only and may be subject to change. A shipping/handling charge of $12.50 per order will be applied. For all inquiries, please email us.
Different modifications, scales, order sizes, oligo length, and purification choices will demand different lengths of synthesis time. Check out our delivery schedules for your configuration needs.
| Type of oligonucleotides | Ships within | Delivery on |
|---|---|---|
| Desalted, unmodified ≤40 bases ordered before 1 pm EST (U.S.) | Same day | 1st business day |
| Desalted, unmodified ≤40 bases ordered before 8 pm EST (U.S.) | 1 business day | 2nd business day |
| Desalted 46–100 bases; phosphorylated oligonucleotides, cartridge purified and phosphorothioate oligonucleotides | 2 business days | 3rd business day |
| 3′ & 5′ modified | 5 business days | 6th business day |
| HPLC and PAGE purity oligonucleotides; 1 µmole scale, 10 µmole scale | 7th business day |
For plate orders containing oligos of 50 bases or less.
| Number of plates | Ships within | Delivery on |
|---|---|---|
| 1–4 plates | 3 business days | 4th business day |
| 5–8 plates | 4 business days | 5th business day |
| 9–14 plates | 6 business days | 7th business day |
| 15–21 plates | 8 business days | 9th business day |
| 21–35 plates | 10 business days | 11th business day |
| 36 or more plates | Inquire | |
For any 3′ or 5′ modifications, add 2 business days to each category.
For plate orders containing oligos greater than 50 bases in length, add 2 business days to each category.
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For Research Use Only. Not for use in diagnostic procedures.