Designed for oligonucleotide synthesis for diagnostic and therapeutic development

Thermo Scientific RNA phosphoramidites are built to meet the varying needs of oligonucleotide manufacturers for the development of therapeutic, diagnostic, and research applications. We offer TheraPure, standard, and fast deprotect phosphoramidites. 

  • TheraPure phosphoramidites undergo additional quality control release testing and are compared with our standard phosphoramidites, helping to ensure that impurities and residual solvents are controlled to the stringent levels required by our customers for their oligo therapeutics.
  • Standard phosphoramidites support the quality and reliability suitable for routine oligonucleotide synthesis.
  • Fast deprotect phosphoramidites allow the removal of base protecting groups under mild or accelerated conditions without the need for special reagents. 

Our RNA phosphoramidites are supported by:

  • Expert technical support—over 40 years of partnership, customization, and scale-up experience 
  • Secure and consistent supply—5x capacity expansion in 2022 
  • Service excellence—48 hours or less response to orders on standard business days, >98% delivery to customer at promised date†
  • Flexible packaging—custom and bulk packaging available upon request
  • Quality—ISO 9001:2015–certified quality management system that has been refined in partnership with our customers since 1995
†May not be applicable in all regions.

Quality manufacturing and regulatory documentation

Processes are monitored from material procurement through final QC and product release for batch-to-batch reproducibility. Purity of each phosphoramidite lot is assessed by HPLC-MS, GC, 31P NMR, as well as Karl Fischer oven titration. Our rigorously controlled manufacturing processes help minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide during synthesis and result in the accumulation of by-products.

Available RNA phosphoramidites by synthesizer

  Applied Biosystems/AKTA SynthesizerExpedite SynthesizerMerMade SynthesizerBulk
TheraPure phosphoramiditesBz-rA  ✓✓✓✓
Ac-rC✓✓✓✓
iBu-rG  ✓✓✓✓
rU✓✓✓✓
Standard phosphoramiditesBz-rA  ✓   
Ac-rC✓   
iBu-rG  ✓   
rU✓   
Fast deprotection (mild)PAC-rA✓✓  
iPrPAC-rG✓✓  
Ac-rGContact us

For items not listed above, flexible/bulk packaging, or custom services, please contact us ›

TheraPure RNA Phosphoramidites

TheraPure phosphoramidites are manufactured with high purity and tight control of critical impurity level for excellent batch-to-batch reproducibility.

Chemical structures and technical specifications

  Click images to enlarge

 Bz-rA CEPiBu-rG CEPAc-rC CEPrU CEP
Cat. No. 27-190327-190627-180527-1804
FormulaC53H66N7O8PSiC50H68N7O9PSiC47H64N5O9PSiC45H61N4O9PSi
Molecular weight

988.2 g/mol970.1 g/mol902.1 g/mol861.1 g/mol
CAS #104992-55-4147201-04-5121058-88-6118362-03-1
AppearanceColor intensity must not exceed Pantone #P1-4U or #P4-2U
Solution clarity  Free from undissolved particulate at 0.2 M solution in ACN
HPLC purity≥99%
31P NMR purity≥99%
Residual solvent≤3.0% total % by weight solvent residue
Shipping / Storage conditionAmbient / –20°C
Water content ≤0.3%
Material originNon-animal source (no TSE/BSE risk)

Ordering information

Applied Biosystems and AKTA synthesizer (serum vial) compatible products
Expedite synthesizer compatible products
MerMade synthesizer compatible products
Bulk RNA phosorphoramidites

Not finding the product you need? Please contact us or request custom services.

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Standard RNA Phosphoramidites

Traditional base protection methods are used to help ensure quality and reliability for routine oligonucleotide synthesis.

Chemical structures and technical specifications

  Click images to enlarge

 Bz-rA CEPiBu-rG CEPAc-rC CEPrU CEP
Cat. No.27-140327-140627-140527-1404
FormulaC53H66N7O8PSiC50H68N7O9PSiC47H64N5O9PSiC45H61N4O9PSi
Molecular weight

988.2 g/mol970.1 g/mol902.1 g/mol861.1 g/mol
CAS #104992-55-4147201-04-5121058-88-6118362-03-1
AppearanceColor intensity must not exceed Pantone #P1-4U or #P4-2U
HPLC purity≥98.0%
31P NMR purity ≥98.0%
Shipping / Storage conditionAmbient / –20°C
Water content ≤0.3%
Material originNon-animal source (no TSE/BSE risk)

Ordering information

Applied Biosystems and AKTA synthesizer (serum vial) compatible products

Not finding the product you need? Please contact us or request custom services.

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Fast deprotect RNA phosphoramidites

They are ideal for applications requiring mild deprotection to help preserve the integrity of oligonucleotides containing sensitive dyes, labels, or other modifications that may be unstable with prolonged exposure to strong alkaline conditions. Fast deprotect phosphoramidites permit the removal of base protecting groups under mild or accelerated conditions without the need for special reagents. 

Chemical structures and technical specifications

  Click images to enlarge

 TheraPure PAC-rA CEP *TheraPure iPrPAC-rG CEP *TheraPure Ac-rG CEP **
Cat. No.27-180327-1806SY3000
FormulaC54H68N7O9PSiC57H74N7O10PSiC48H64N7O9PSi
Molecular weight1018.2 g/mol1076.3 g/mol942.14 g/mol
CAS #121058-86-4956580-73-7944138-03-8
Solution clarity0.2 M solution in ACN is free from undissolved particulate
HPLC purity≥99%
31P NMR purity≥99%
Water content ≤0.3%
Shipping / Storage conditionAmbient / –20°C
Material origin Non-animal source (no TSE/BSE risk)
942.14 g/mol
942.14 g/mol

* When using both of PAC-rA (Cat No. 27-1803) and iPrPAC-rG (Cat No. 27-1806), select Ac-rC (Cat No. 27-1805) and rU (Cat No. 27-1804) to minimize the time for post-synthesis deprotection.

** The acetyl-protected rG (Ac-rG, Cat No. SY3000) amidite is a suitable replacement for iBu-rG (Cat No. 2706) and is ideal for synthesis of longmers. Oligonucleotide deprotection times can be reduced as much as 25%. 

Ordering information

Applied Biosystems and AKTA synthesizer (serum vial) compatible products
Expedite synthesizer compatible products

Not finding the product you need? Please contact us or request custom services.

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Resources

For Research Use Only. Not for use in diagnostic procedures.