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Accelerate Your PCR and qPCR Workflow
Maximize real-time PCR results by pairing PowerTrack SYBR Green Master Mix with either SuperScript IV VILO reverse transcriptase for superior cDNA synthesis or SuperScript IV CellsDirect cDNA synthesis kit to go from mammalian cells all the way to first-strand cDNA synthesis without having to isolate RNA. These best-in-class reagents were designed together to help accelerate your research by offering accurate data quickly.
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The Invitrogen SuperScript IV VILO Master Mix is a first-strand cDNA synthesis reaction mix for two-step RT-qPCR. The master mix format elevates the trusted VILO technology (Variable Input, Linear Output) to the next level by combining further optimized buffer conditions with highly processive and thermostable Invitrogen SuperScript IV Reverse Transcriptase (RT). The master mix offers exceptional performance features while maintaining superior linearity across the broadest range of input RNA SuperScript IV VILO Master Mix for reverse transcriptase features:
- Super-efficient–up to 100x higher cDNA yield
- Super-sensitive–Ct values reduced by as many as 8 cycles for RT-qPCR
- Super-robust–transcribes even from degraded or inhibitor-containing RNA samples
- Super-fast–10 min reaction time
The SuperScript IV CellsDirect cDNA Synthesis kit is designed to synthesize first-strand cDNA directly from mammalian cell lysates without requiring the initial isolation of RNA. Lysis and reverse transcription are performed in the same tube. The resulting first-strand cDNA is ready to use in downstream applications including PCR and qPCR. This cDNA synthesis kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell. The SuperScript IV CellsDirect cDNA Synthesis kit features:
- Time-saving–save up to two hours in overall workflow time
- Streamlined workflow–lysis and RT reaction times reduced and completed in a single tube
- Superior sensitivity–easy detection of low abundant targets
- Direct convenience–no need to isolate RNA and store in your freezer
The PowerTrack SYBR Green Master Mix is a flexible, easy-to-use gene expression master mix for real-time PCR. The PowerTrack SYBR Green Master Mix features:
- Two-color tracking dye system to indicate where pipetting has occurred.
- Broad primer Tm and primer concentration compatibility for flexibility in qPCR reaction setup with minimal optimization.
- Superior specificity and tight reproducibility in Ct values over a broad dynamic range to improve data quality.
- Compatibility with SuperScript IV VILO master mix reverse transcription for fast, reproducible results.
- UNG/dUTP to prevent contamination from carryover PCR products.
- Broad instrument compatibility.
The Invitrogen SuperScript IV VILO Master Mix is a first-strand cDNA synthesis reaction mix for two-step RT-qPCR. The master mix format elevates the trusted VILO technology (Variable Input, Linear Output) to the next level by combining further optimized buffer conditions with highly processive and thermostable Invitrogen SuperScript IV Reverse Transcriptase (RT). The master mix offers exceptional performance features while maintaining superior linearity across the broadest range of input RNA SuperScript IV VILO Master Mix for reverse transcriptase features:
- Super-efficient–up to 100x higher cDNA yield
- Super-sensitive–Ct values reduced by as many as 8 cycles for RT-qPCR
- Super-robust–transcribes even from degraded or inhibitor-containing RNA samples
- Super-fast–10 min reaction time
The SuperScript IV CellsDirect cDNA Synthesis kit is designed to synthesize first-strand cDNA directly from mammalian cell lysates without requiring the initial isolation of RNA. Lysis and reverse transcription are performed in the same tube. The resulting first-strand cDNA is ready to use in downstream applications including PCR and qPCR. This cDNA synthesis kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell. The SuperScript IV CellsDirect cDNA Synthesis kit features:
- Time-saving–save up to two hours in overall workflow time
- Streamlined workflow–lysis and RT reaction times reduced and completed in a single tube
- Superior sensitivity–easy detection of low abundant targets
- Direct convenience–no need to isolate RNA and store in your freezer
The PowerTrack SYBR Green Master Mix is a flexible, easy-to-use gene expression master mix for real-time PCR. The PowerTrack SYBR Green Master Mix features:
- Two-color tracking dye system to indicate where pipetting has occurred.
- Broad primer Tm and primer concentration compatibility for flexibility in qPCR reaction setup with minimal optimization.
- Superior specificity and tight reproducibility in Ct values over a broad dynamic range to improve data quality.
- Compatibility with SuperScript IV VILO master mix reverse transcription for fast, reproducible results.
- UNG/dUTP to prevent contamination from carryover PCR products.
- Broad instrument compatibility.
For Research Use Only. Not for use in diagnostic procedures.