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Search Thermo Fisher Scientific
We offer a variety of activated supports and accessories for the immobilization of proteins, antibodies, and other molecules. These resins are available separately or in convenient kits. Choose resins based on NHS-ester, AminoLink™, SulfoLink™, GlycoLink™, CarboxyLink™, and other activation technologies.
Pierce NHS-Activated Agarose | AminoLink Coupling Resin | AminoLink Plus Coupling Resin | SulfoLink Coupling Resin | GlycoLink Coupling Resin | CarboxyLink Coupling | |
Target | -NH2 | -NH2 | -NH2 | -SH | -CHO | -COOH |
Support | 6% agarose (slurry or dry powder) | 4% agarose | 4% agarose, highly crosslinked | 6% agarose | Ultralink (polyacrylamide/ azlactone copolymer) | 4% agarose |
Coupling capacity | >25 mg/mL resin | 1–20 mg/mL resin | 20 mg/mL resin | 20 mg/mL resin | 1–10 mg/mL resin | 5 mg/mL resin |
Coupling time | 30 min | 4 hr | 4 hr | 2–3.5 hr | 30 min | 4 hr |
Recommended for | Proteins | Proteins, antibodies | Proteins, antibodies | Proteins, peptides, antibodies | Glycoproteins, polyclonal antibodies | Unmodified peptides |
Advantages | High coupling efficiency and capacity | High coupling efficiency | High coupling efficiency | Allows for gentle elution | Correctly orients antibodies during coupling | Flexible and gentle coupling conditions |
Disadvantages | Narrow pH range (7–9) | May be coupled at antigen-binding site | May be coupled at antigen-binding site | Protein must be reduced first | Not recommended for monoclonal antibodies | Not recommended for antibodies |
26196, 26197 (dry resin), 26200 (slurry) | 20381 (10 mL), 20382 (50 mL) | 20501 (10 mL), 20505 (50 mL) | 20401 (10 mL), 20402 (50 mL), 20404 (250 mL) | 53149 (10 mL) | 20266 (25 mL) |
Immobilization efficiencies for various proteins on 1 mL of AminoLink Plus Resin when immobilized using the pH 7.2 coupling procedure.
Protein | Protein (mg) reacted | Protein (mg) coupled | Coupling efficiency (%) |
---|---|---|---|
Protein G | 4.6 | 4.0 | 83 |
Mouse IgG | 4.7 | 4.5 | 96 |
Rat IgG | 4.7 | 4.4 | 93 |
GAM*-IgG | 0.9 | 0.8 | 84 |
Human IgG | 4.8 | 4.6 | 97 |
Human IgM | 0.9 | 0.8 | 93 |
*GAM-IgG is goat anti-mouse IgG, a secondary antibody.
Additional activated supports and accessories are available, as well as convenient kit formats that included disposable columns, buffers, and coupling reagents. Some activated supports are also available in magnetic bead formats.
For Research Use Only. Not for use in diagnostic procedures.