Cell dissociation

Our cell dissociation reagents help you prepare a desired number of cells and plate them so that they are healthy, proliferating, and in a desired confluency at the time of transfection.

Select cell dissociation reagent type

For adherent cell culture, choose from trypsin and trypsin alternatives to dissociate cultured cells into single cells.

Trypsin-EDTA, phenol red

Trypsin with phenol red in 100 ml bottle
  • Fast cell dissociation
  • General purpose uses
TrypLE Express Enzyme
100 mL TrysinLE Express bottle
  • Gentle, convenient
  • Animal origin-free
  • Inhibition by dilution (no inhibitors needed)
Balanced salt solutions
500 mL bottle of phosphate buffered saline (PBS)
  • High quality and consistency for reproducible results
  • Manufactured in cGMP, ISO-certified facilities
  • Tested for osmolality, pH, stability, bacterial and fungal contamination, and endotoxins

Cell counting

Choose from manual and automated options to quickly and accurately count cells while minimizing user variation associated with counting.

Select manual or automated counting method

Trypan blue solution

100 mL bottle of 0.4% trypan blue stain
  • Determine cell viability quickly and accurately
  • Use with the hemocytometer or with the Countess chamber slides

The Invitrogen Countess 3 Automated Cell Counter uses advanced machine-learning image analysis algorithms to deliver accurate cell counts and viability in less than 30 seconds. Find disposable Countess Cell Counting Chamber Slide, the Countess Reusable Slide, stains and other accessories for Countess 3 Automated Cell Counter.

Countess 3 Automated Cell Counter
Pro tip

Use an automated cell counter to generate fast and highly accurate cell counts even for challenging cell sample types.

Learn more

Cell plating

Choose culture dishes, media, sera, and supplements to plate your cells and get them ready for transfection.

Select cell plating product type

Nunc Cell-Culture Treated Dishes and Plates

4-well, 6-well, 12-well, 24-well, and 48-well clear cell culture dishes
  • Multi-well plates with the Nunclon Delta surface treatment (proprietary energy source, not coating) for more hydrophilic surface and better cell attachment
  • Tested with at least four cell lines
BenchStable Cell Culture Media
500 mL BenchStable DMEM medium bottle with its packaging
  • Enable storage at room temperature for flexibility and convenience
  • Available in commonly used basal media formulations
  • High quality and consistency for results you can rely on
Fetal Bovine Serum, Premium
500 mL bottle of FBS certified US origin
  • Tested for up to 90 quality specifications, including 9 CFR virus testing, endotoxin, and performance
  • Consistent reliability, service, value, and innovation since 1962

Glutamine, glucose, pyruvate, etc.

100 ml bottle of 100X GlutaMAX-I
  • High quality, consistency, and performance from the most basic formulations to the newest innovations

Need more information?

Successful transfection is influenced by many factors—the choice of the transfection method, health and viability of the cell line, number of passages, degree of confluency, quality and quantity of the nucleic acid used, and the presence or absence of serum in the medium can all play a part in the outcome of your transfection experiment. While it is possible to optimize specific transfection conditions to achieve high transfection efficiencies, it is important to note that some cell death is inevitable regardless of the transfection method used.

For Research Use Only. Not for use in diagnostic procedures.