Three-Parameter Population Analysis Right From Your Bench

We’ve just released the Tali™ Image-Based Cytometer—a 3-channel imaging platform that performs suspension cell–based assays right from your bench. With the Tali™ cytometer, three-parameter population analysis can be completed in approximately 1 minute per sample using 25 μL sample volumes. You can now quickly and efficiently measure cell viability or apoptosis in a population of suspension cells. The Tali™ cytometer also allows you to quantitate protein expression in GFP- or RFP-containing cells.

Versatility For Multiple Applications

In addition to a bright-field channel, the Tali™ Image-Based Cytometer features two fluorescence channels (green and red), enabling it to count green- and red-fluorescent stained cells, including cells expressing GFP and RFP. The Tali™ Image-Based Cytometer displays comprehensive data with graphical reports, allows the export of data for archiving and sample comparisons, and provides sufficient cell analysis for statistical significance.

If you are a researcher who works with cultured mammalian, primary, or insect cells, you can now measure fluorescent protein (GFP or RFP) expression (Figure 1), cell viability, and apoptosis (using fluorescent annexin V conjugates) (Figure 2) at your bench and at any time. If you are a flow cytometer user, the Tali™ Image-Based Cytometer also allows you to quickly check the concentration and fluorescence of your samples before analysis or sorting on flow instruments to improve efficiency. Thirteen cell types have already been tested with the Tali™ cytometer: 293 MSR, A431, A549, BPAEC, CHO-M1, HASMC, HDFN, HeLa, HEKn, JKT, MMM, NIH-3T3, and U2OS.
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Molecular Probes Handbook 11th edition

The Tali™ GFP and RFP assay 
Figure 1. The Tali™ GFP and RFP assay. The Tali™ Image-Based Cytometer has two fluorescent channels (green and red), enabling it to quantitate green and red fluorescence. It also provides actual cell counts and automatically calculates cell concentration for each cell population.
Tali™ Apoptosis Kit 
Figure 2. Apoptosis assay with the Tali™ cytometer. The Tali™ Apoptosis Kit provides Alexa Fluor® 488 annexin V and propidium iodide (PI) to assess cell viability and health in a population of suspension cells.

Fast and Easy, With a Small Footprint

With the Tali™ Image-Based Cytometer, prescreening samples for flow experiments, visualizing cells, and obtaining cell measurements is fast, easy, and convenient. Moreover, with its small footprint, the Tali™ cytometer can easily fit into your lab’s work space. Learn more about the Tali™ Image-Based Cytometer.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.