Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
This kit combines a novel two-color fluorescent probe for yeast viability, FUN 1, with a fluorescent fungal surface labeling reagent, Calcofluor White M2R. Plasma membrane integrity and metabolic function of fungi are required to convert the yellow-green fluorescent intracellular staining of FUN 1 into red-orange intravacuolar structures; Calcofluor white M2R labels cell-wall chitin with blue-fluorescence, regardless of metabolic state.
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FUN 1 cell stain | Calcofluor white M2R | |
---|---|---|
Excitation/Emission | 488/530 nm | 365/435 nm |
Standard filter set | FITC | DAPI |
Storage conditions | ≤20°C, protect from light | ≤20°C, protect from light |
Saccharomyces cerevisiae stained with FUN 1 cell stain, which generates red-fluorescent intravacuolar structures, and with Calcofluor white M2R, a blue-fluorescent cell wall stain.