Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Contents: (concentrations in parenthesis) | Final concentrations: |
---|---|
Fetal Bovine Serum | (5%) |
Dexamethasone in DMSO (10 mM) | (1 μM) |
Thawing/Plating Cocktail — A, containing (18 mL total volume): | |
| (1%) |
| (4 μg/ml) |
| (2 mM) |
| (15 mM) |
For use with: Williams E Medium (WEM, A1217601)
Applications: Incubation medium for cells in suspension, maintenance of cultured hepatocytes
Contents: (concentrations in parenthesis) | Final concentrations: |
---|---|
Dexamethasone in DMSO (10 mM) | (0.1μM) |
Cell Maintenance Cocktail — B, containing (20 mL total volume): | |
| (0.5%) |
| (6.25 μg/ml) (6.25 μg/ml) (6.25 ng/mL) (1.25 mg/ml) (5.35 μg/mL) |
| (2 mM) |
| (15 mM) |
Directions for supplementing medium * :
To 500 ml medium, add:
* To extend life of these products, we recommend supplementing small aliquots of medium just prior to use. Remaining medium, cocktail or dexamethasone should be stored at 4°C for up to twelve months or up to expiration date listed on bottle. Remaining serum should be stored at -20°C.
Table 1 — Volumes of cocktail A and supplements for supplementing aliquots.
WEM | Cocktail A | FBS | Dex |
50 mL | 1.8 mL | 2.5 mL | 5 μL |
100 mL | 3.6 mL | 5 mL | 10 μL |
200 mL | 7.2 mL | 10 mL | 20 μL |
300 mL | 10.8 mL | 15 mL | 30 μL |
400 mL | 14.4 mL | 20 mL | 40 μL |
500 mL | 18 mL | 25 mL | 50 μL |
Table 2: Volumes of cocktail B and supplements for supplementing aliquots.
WEM | Cocktail | B Dex |
---|---|---|
50 mL | 2 mL | 0.5 μL |
100 mL | 4 mL | 1 μL |
200 mL | 8 mL | 2 μL |
300 mL | 12 mL | 3 μL |
400 mL | 16 mL | 4 μL |
500 mL | 20 mL | 5 μL |
For Research Use Only. Not for use in diagnostic procedures.