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SYBR Safe™ DNA Gel Stain
SYBR Safe™ DNA gel stain has been specifically developed for reduced mutagenicity, making it safer than ethidium bromide for staining DNA in agarose gels. The detection sensitivity of E-Gel® with SYBR Safe™ stain is similar to that of E-Gel® containing ethidium bromide. DNA bands stained with SYBR Safe™ DNA gel stain can be detected using a standard UV transilluminator, a visible-light transilluminator or a laser-based scanner.
Safety Features of SYBR Safe™
Disposal of SYBR Safe™
SYBR Safe™ DNA gel stain is not classified as hazardous waste, but as disposal regulations vary, please contact your safety office or local municipality for appropriate SYBR Safe disposal in your community.
Spectrum of SYBR Safe™ Bound to nucleic acids, SYBR Safe™ stain has fluorescence excitation maxima at 280 and 502 nm, and an emission maximum at 530 nm (see figure below).
Normalized fluorescence excitation and emission spectra of SYBR Safe™ DNA gel stain, determined in the presence of DNA
Visualization of SYBR Safe™
Detect DNA bands stained with SYBR Safe™ DNA gel stain using a blue-light transilluminator, a standard UV transilluminator, or a laser-based scanner. For photographing gels, a special filter may be required.
Cloning Benefits of SYBR Safe™
Using the SYBR Safe™-blue-light method dramatically reduces DNA damage. As a result, cloning efficiency can improve ten- to thousand-fold.
Introduction
The E-Gel® agarose gel electrophoresis system is a complete bufferless system for agarose gel electrophoresis of DNA samples.
The major components of the system are:
E-Gel® pre-cast agarose gels are self-contained gels that include electrodes packaged inside a dry, disposable, UV-transparent cassette. The E-Gel® agarose gels run in a specially designed device that is a base and power supply combined into one device. They either contain SYBR Safe™ DNA gel stain, ethidium bromide, or no DNA gel stain.
Advantages of E-Gel®
Using E-Gel® agarose gels for electrophoresis of DNA samples offer the following advantages:
Available DNA Gel Stains
E-Gel® agarose gels come in three formats for staining your DNA:
SYBR Safe™ DNA gel stain offers the following advantages:
Throughput Formats
Two types of E-Gel® agarose electrophoresis systems are available from Invitrogen based on your throughput requirements.
Low-Throughput E-Gel Electrophoresis System
The Low-Throughput E-Gel® Electrophoresis System is designed for electrophoresis of 12-16 DNA samples per gel. The system consists of the following components:
The Medium/High-Throughput E-Gel® Electrophoresis System
The Medium/High-Throughput E-Gel® Electrophoresis System is designed for electrophoresis of 48-96 DNA samples per gel. This system is compatible for use with multichannel pipettors or automated liquid handling systems.
The system consists of the following components:
E-Gel® 48 gels
E-Gel® 96 gels
E-Base™ Electrophoresis Device
E-Holder™ Platform
E-Editor™ 2.02 Software
Choosing a Gel for Your Application
To obtain the best results for your application, it is important to choose the correct agarose percentage and well format.
The table below lists the various types of gel and resolution for each gel type.
Gel Type
|
No. Rows
|
No. Wells
|
Sample Volume
|
Run Length
|
Run Time
|
% Agarose
|
Resolution
|
---|---|---|---|---|---|---|---|
E-Gel
® with SYBR Safe™
|
1
|
12
|
10-20 µl
|
5.8 cm
|
30 min.
|
1.2%
2%
|
100 bp-5 kb
100 bp-2 kb
|
E-Gel
® single comb
|
1
|
12
|
10-20 µl
|
5.8 cm
|
30 min.
|
0.8%
1.2%
2%
4%
|
800 bp-10 kb
100 bp-5 kb
100 bp-2 kb
20 bp-500 bp
|
E-Gel
® double comb
|
2*
|
16 sample
2 marker
|
10-20 µl
10 µl
|
2.9 cm
|
15 min.
|
0.8%
2%
|
1 kb-10 kb
100 bp-2 kb
|
Clear E-Gel
® |
1
|
12
|
20 µl
|
5.8 cm
|
30 min.
|
1.2%
|
100 bp-5 kb
|
E-Gel
® 48 Gel
|
2*
|
48 sample
4 marker
|
10-15 µl
15 µl
|
3.2 cm
|
20 min.
|
1%
2%
4%
|
400 bp-10 kb
50 bp-3 kb
10 bp-400 bp
|
E-Gel
® 96 Gel
|
8*
|
96 sample
8 marker
|
10-20 µl
20 µl
|
1.6 cm
|
12 min.
|
1%
2%
|
1 kb-10 kb
100 bp-2 kb
|
*Wells compatible for loading with a multichannel pipettor.
System Components
The Low-Throughput E-Gel® Electrophoresis System consists of the following components:
Note: The E-Gel® Base previously available from Invitrogen can be used for electrophoresis of E-Gel® with SYBR Safe™, E-Gel® single comb, double comb, and Clear E-Gel® agarose gels.
Applications
E-Gel® agarose gels are suitable for analyzing:
When to Use E-Gel® With SYBR Safe™
When to Use E-Gel® With SYBR Safe®
Clear E-Gel® agarose gels do not contain ethidium bromide in the gels and are ideal for the following applications:
E-Gel® Single Comb and Double Comb Gels
The E-Gel® single comb and double comb gels are bufferless gels containing electrodes embedded in the agarose matrix. Each gel contains an ion generating system (TAE buffer system), a pH balancing system, and ethidium bromide for DNA staining and is packaged inside an UV-transparent cassette.
To create a patented bufferless system, each E-Gel® single comb and double comb cassette contains two ion exchange matrices (IEMs) that are in contact with the gel and electrodes. The IEMs supply a continuous flow of ions through out the gel resulting in a sustained electric field required for running the gel (see figure below).
Clear E-Gel® agarose gels are single comb gels that do not contain any nucleic acid stain in the agarose.
E-Gel® PowerBase™
The E-Gel® PowerBase™ Version 4 (figure below) is an easy-to-use, automated device specifically designed to simplify electrophoresis of single comb or double comb E-Gel® agarose gels from Invitrogen. The E-Gel® PowerBase™ is a base and a power supply all in one device.
The operation of the E-Gel® PowerBase™ v. 4 is controlled by two buttons on top of the base. The left button is for a double comb run and right button is for a single comb run (see the label on the unit). To select different electrophoresis runs for the PowerBase™, do one of the following.
E-Gel® Base
The E-Gel® Base (see figure below) previously available from Invitrogen connects to a power supply and is used for electrophoresis of E-Gel® single comb, double comb, and Clear E-Gel® agarose gels.
E-Gel® Opener
The E-Gel® Opener is an easy-to-use device specifically designed to open any E-Gel® single comb, double comb, or Clear E-Gels® for excision of DNA fragments or for blotting.
Cassette Size: | 13.5 cm (l) x 10.8 cm (w) x 0.67 cm (thick) |
Gel Thickness: | 3.7 mm |
Gel Volume: | 50 ml |
Gel Percentage: | 1%, 2%, and 4% |
Well Depth: | 3 mm |
Dimensions of the Well: | 3.6 mm (l) x 2.2 mm (w) |
Running Distance (one well to the next): | 32 mm |
Space between Well Center: | 4.5 mm |
Cassette Size: | 13.5 cm (l) x 10.8 cm (w) x 0.67 cm (thick) |
Gel Thickness: | 3.7 mm |
Gel Volume: | 50 ml |
Gel Percentage: | 1% & 2% |
Well Depth: | 3 mm |
Well Opening: | 3.8 mm x 1.8 mm |
Well Bottom: | 3.3 mm x 1.1 mm |
Running Distance (one well to the next): | 16 mm |
Space between Wells: | 9 mm |
Dimensions: | 14.6 cm x 15 cm x 5.3 cm |
Weight: | Mother E-Base™- 370 g Daughter E-Base™- 271 g |
Safety: | Double Insulation, UL listed, and CE certified |
Temperature: | Ambient 15°C to 40°C |
Built-in Features: | Digital time display (00-99 minutes), alarm, light LED |
Dimensions: | 12.5 cm x 13 cm x 13.5 cm |
Weight: | 1.19 lbs (540 g) with adaptor |
Safety: | UL listed and CE certified |
Temperature: | Ambient 15°C to 40°C |
Built-in Features: | alarm, light LED |
Country | Input | Output |
---|---|---|
U.S. and Canada | 110-120 V AC, 60 Hz12 V DC, 880 mA | 12 V DC, 880 mA|
Europe | 220-240 V AC, 50 Hz | 12 V DC, 880 mA |
Dimensions: | 12.5 x 13 x 3.5 cm |
Weight: | 3.18 oz. (90 g) |
Temperature: | Ambient 15C to 40°C |
Introduction
For optimal results, follow the guidelines for preparing your DNA sample as described in this section.
Materials Needed
Amount of DNA
Use 20-100 ng DNA per band for samples containing one unique band, or up to 500 ng per lane (E-Gel® 1.2% with SYBR Safe™) or 700 ng per lane (E-Gel® 2% with SYBR Safe™) of samples containing multiple bands. If you are unsure how much to use, test a range of concentrations to determine the optimal concentration for your particular sample. Excess DNA will cause poor resolution.
Loading Method
The DNA samples are loaded in E-Gel® agarose gels using the One-Step Loading or Two-Step Loading method.
The One-Step Loading method is routinely used to load E-Gel® agarose gels.
The Two-Step Loading method is used to load E-Gel® agarose gels, if the One-Step Loading method produces fuzzy or indistinct bands, or the gel was removed from its plastic pouch for more than 15 minutes.
Prepare loading buffer and sample volumes based on the loading method as described below.
Total Sample Volume
The recommended total sample volume for each gel type is listed in the table below.
Note: For best results, keep all sample volumes uniform. If you do not have enough samples to load all wells of the gel, load an equal volume of deionized water into any empty wells.
Gel Type | One-Step Loading | Two-Step Loading |
E-Gel
® with SYBR Safe
™ |
20 µl
|
10 µl
|
Loading Buffer
High Salt Samples
Important: To obtain the best results, prepare your high salt samples as described below.
Samples containing > 20 mM NaCl or 5 mM EDTA (i.e. certain restriction enzyme and PCR buffers) will cause loss of resolution on E-Gel® agarose gels. Dilute samples 2- to 20-fold as described:
One-Step Loading | Two-Step Loading |
Dilute samples with the loading buffer, deionized water, or TE to a final volume of 20 µl
|
Dilute samples with loading buffer, deionized water, or TE buffer to obtain a final sample volume of 10 µl (10% glycerol may be added)
|
Preparing Samples
Prepare your samples based on the loading method used as described below:
One-Step Loading | Two-Step Loading | |
Total Sample Volume
|
Add deionized water to the required amount of DNA to bring the total sample volume to 20 µl.
|
Mix the required amount of DNA with deionized water to obtain a total sample volume of 10 µl (10% glycerol may be added). When loading into the gel, first load 10 µl of deionized water directly into each well and then add 10 µl of sample.
|
Loading Buffer
|
Optional: Instead of water, you may use a loading buffer that yields a final concentration of 10 mM Tris-HCl, 1 mM EDTA, pH 7.5; 0.005% bromophenol blue; and 0.005% xylene cyanol FF.
To use 10X BlueJuice™TrackIt™ Loading Buffer, dilute this buffer 50- to 200-fold to obtain the optimal dye concentration.
|
Optional: Instead of water, you may use a loading buffer that yields a final concentration of 10 mM Tris-HCl, pH 8.1; 1 mM EDTA, pH 7.5; 0.005% bromophenol blue; 0.005% xylene cyanol FF (10% glycerol may be added).
To use 10X BlueJuice
™TrackIt™ Loading Buffer, dilute this buffer 50- to 200-fold to obtain the optimal dye concentration.
|
DNA Molecular Weight Markers
We recommend using the following DNA molecular weight markers for different types of E-Gel® agarose gels to obtain good resolution.
Note: Supercoiled DNA molecular weight markers may produce a slightly fuzzy pattern when run on E-Gel® with SYBR Safe™ agarose gels. Consider using Clear E-Gel® agarose gels and post-staining with SYBR Safe™ DNA Gel Stain.
Product | Markers | Catalog no. | Amount Used |
E-Gel
® 1.2% with SYBR Safe
™ |
TrackIt
™ 100 bp DNA Ladder
|
10488-058
|
Load 500-700 ng markers in a volume of 20 µl.
|
TrackIt
™ 1 Kb Plus DNA Ladder
|
10488-085
| ||
100 bp DNA Ladder
|
15628-019
| ||
1 Kb Plus DNA Ladder
|
10787-018
| ||
High DNA Mass Ladder
|
10496-016
| ||
E-Gel
® High Range DNA Marker
|
12352-019
| ||
E-Gel
® 2% with SYBR Safe
™ |
TrackIt
™ 25 bp DNA Ladder
|
10488-022
| |
TrackIt
™ 50 bp DNA Ladder
|
10488-043
| ||
25 bp DNA Ladder
|
10597-011
| ||
50 bp DNA Ladder
|
10416-014
| ||
100 bp DNA Ladder
|
15628-019
| ||
Low DNA Mass Ladder
|
10068-013
| ||
E-Gel
® Low Range Quantitative DNA Marker
|
12373-031
|
Introduction
After you have prepared your samples, you are ready to proceed with electrophoresis. Instructions are provided below to load and run E-Gel® with SYBR Safe™ (which come as single comb gels) using the E-Gel® PowerBase™ v.4.The total run times are 30–33 minutes.
Pre-running E-Gel® with SYBR Safe™
You must first pre-run the E-Gel® with SYBR Safe™ agarose gel for 2 minutes with the comb in place before loading your samples to ensure proper resolution of your DNA fragments.
Each E-Gel® cassette is supplied individually wrapped and ready for use. To set up and use an E-Gel® with SYBR Safe™ gel, follow the instructions below:
Method of Loading Samples
The E-Gel® with SYBR Safe™ agarose gels are designed for loading samples manually using either the One-Step Loading method or the Two-Step Loading method. The One-Step Loading method is routinely used to load E-Gel® with SYBR Safe™ gels; use the Two-Step Loading method, if the One-Step Loading method produces fuzzy or indistinct bands, or the gel was removed from its plastic pouch for more than 15 minutes.
One-Step Loading Method
All wells in the gel must contain sample or water. Avoid introducing bubbles while loading, as bubbles will cause bands to distort.
Alternate Method:
Two-Step Loading
All wells in the gel must contain sample or water. Avoid introducing bubbles while loading, as bubbles will cause bands to distort.
Introduction
The E-Gel® Opener is an easy-to-use device specifically designed to open any E-Gel® single comb or double comb gels for excision of DNA fragments or for blotting.
Do not use the E-Gel® Opener to open the E-Gel® 48 or 96 cassettes. The E-Gel® 48 or 96 cassette cannot be opened.
Description of Opener
The E-Gel® Opener consists of an anodized aluminum platform housing two recessed steel blades, one which is stationary and one which is movable. The blades are brought into contact with the E-Gel®cassette by turning the large knob clockwise (see Figure 1).
Before using the E-Gel® Opener for the first time, we recommend that you practice opening a few used E-Gels® to familiarize yourself with the process. Practice on E-Gels®that will not be used further for preparative purposes.
The blades on the E-Gel® Opener are extremely sharp. Do not insert your fingers into the area housing the blades! Pick up the E-Gel® Opener by holding the large knob only (see Figure 1 above). Exercise caution when handling and cleaning the E-Gel®Opener. Dispose of blades in a needle disposal container or a Sharps disposal box.
Procedure
The following section provides instructions to open an E-Gel®cassette. Before beginning, you should wear safety goggles and gloves.
If you plan to purify DNA from the gel, lift off the top of the gel cassette and excise the gel fragment. Transfer the gel slice to a microcentrifuge tube.
Cleaning and Storage
After use, clean the E-Gel® Opener with mild detergent and water to remove excess agarose and plastic from the platform. Use a squirt bottle and wipe the platform dry with a clean tissue. Do not insert your fingers into the area housing the blades, and do not immerse the E-Gel® Opener in water as the blades may rust. Store the E-Gel® Opener at room temperature.
Disposal of E-Gel® with SYBR Safe™
SYBR Safe™ DNA gel stain shows no or very low mutagenic activity when tested by an independent, licensed testing laboratory, and this stain is not classified as hazardous waste under U.S. Federal regulations. As disposal regulations vary, please contact your safety office or local municipality for appropriate SYBR Safe disposal in your community.
E-Gel® 1.2% with SYBR Safe™
An example of DNA samples run on an E-Gel® 1.2% with SYBR Safe™ is shown below. Samples were loaded in a total volume of 20 µl and visualized on a standard 312 nm UV transilluminator. Photographs were taken using the MiniBis photo documentation system from DNR, and the SYBR Safe™ photographic filter using an exposure time of 1.8 sec.
Lane | Sample |
---|---|
1 | High DNA Mass Ladder (130 ng) |
2 | E-Gel® High Range DNA Marker (200 ng) |
3 | 1Kb PCR product (100 ng) |
4 | 3Kb PCR product (200 ng) |
5 | 9Kb PCR product (200 ng) |
6 | 1Kb plus DNA Ladder (500 ng) |
7 | 1Kb plus DNA Ladder (500 ng) |
8 | pBR322 EcoR1 cut (100 ng) |
9 | pBR322 uncut (100 ng) |
10 | pUC19 EcoR1 cut (50ng) |
11 | E-Gel® High Range DNA Marker (200 ng) |
12 | High DNA Mass Ladder (130 ng) |
E-Gel® 2% with SYBR Safe™
An example of DNA samples run on an E-Gel® 2% with SYBR Safe™ is shown below. Samples were loaded in a total volume of 20 µl and visualized on a standard 312 nm UV transilluminator. Photographs were taken using the MiniBis photo documentation system from DNR, and the SYBR Safe™ photographic filter using an exposure time of 1.8 sec.
Lane | Sample |
---|---|
1 | Low DNA Mass Ladder (470 ng) |
2 | E-Gel® Low Range DNA Marker (350 ng) |
3 | 240bp PCR product (500 ng) |
4 | 317bp PCR product (700 ng) |
5 | 1Kb PCR product (100 ng) |
6 | 100bp DNA Ladder (900 ng) |
7 | 50bp DNA Ladder (700 ng) |
8 | 100bp DNA Ladder (900 ng) |
9 | pUC19 EcoR1 cut (50 ng) |
10 | pUC19 uncut (50 ng) |
11 | E-Gel® Low Range DNA Marker (350 ng) |
12 | Low DNA Mass Ladder (470 ng) |
Examples Using Different Imaging Methods
DNA samples run on an E-Gel® 2% with SYBR Safe™ are shown below, recorded using different imaging methods. Samples were loaded in a total volume of 20 µl and visualized using the indicated transilluminator and filter. Photographs were taken using the MiniBis photo documentation system from DNR.
Standard 312 nm UV transilluminator
Long pass ethidium bromide filter
E-Gel® with SYBR Safe™
Standard 312 nm UV transilluminator
SYBR Safe™ photographic filter
E-Gel® with SYBR Safe™
Blue-light transilluminator
SYBR Safe™ photographic filter
E-Gel® with SYBR™ Safe
Lane | Sample |
---|---|
1 | Low DNA Mass Ladder (470 ng) |
2 | E-Gel® Low Range DNA Marker (350 ng) |
3 | 240bp PCR product (500 ng) |
4 | 317bp PCR product (700 ng) |
5 | 1Kb PCR product (100 ng) |
6 | 100bp DNA Ladder (900 ng) |
7 | 50bp DNA Ladder (700 ng) |
8 | 100bp DNA Ladder (900 ng) |
9 | pUC19 EcoR1 cut (50 ng) |
10 | pUC19 uncut (50 ng) |
11 | E-Gel® Low Range DNA Marker (350 ng) |
12 | Low DNA Mass Ladder (470 ng) |
The table below provides solutions to some problems that you may encounter with E-Gel® with SYBR Safe™ agarose gels.
Problem | Cause | Solution |
No current
|
Copper contacts in the base are damaged due to improper use
|
Make sure the copper contacts in the base are intact.
|
Expired or defective gel cassette
|
Use fresh gel cassette. Use properly stored gels before the specified expiration date.
| |
E-Gel
® with SYBR Safe
™ cassette is not inserted properly into a base
|
Remove cassette and reinsert; a steady red light illuminates on the base when the cassette is correctly inserted and power is on.
| |
Incorrect adaptor used
|
Use only UL Listed Class 2 Direct Plug-in Adaptor included with the E-Gel
® PowerBase
™.
| |
Poor resolution or smearing of bands
|
Sample is overloaded
|
Do not load more than 200 ng of sample DNA per band.
|
High salt concentration
|
Dilute your high-salt samples as described
. | |
Aberrant pre-run step
|
Be sure to pre-run the gel but do not exceed 2 minutes.
| |
Very low volume of sample loaded or sample was not loaded properly
|
Avoid introducing bubbles while loading the samples. Bubbles will cause band distortion.
| |
Load the recommended sample volume based on the gel type and loading method. | ||
For proper band separation, we recommend keeping sample volumes uniform. Load deionized water or TE into any empty wells | ||
Gel was not electrophoresed immediately after sample loading
|
For best results, run the gel within 15 minutes of sample loading. If you cannot run the gel immediately after sample loading, use the Two-Step Loading method
| |
Expired gel used
|
Use properly stored gels before the expiration date.
| |
Longer electrophoresis run time or high current during the run
|
Longer run times cause an increase in the current, resulting in poor band migration or a melted gel. Do not run the gel longer than recommended time for each gel type.
| |
Melted gel
|
Increased current due to longer run times
|
Do not run the gel longer than 40 minutes.
|
Sample leaking from the wells
|
Sample is overloaded
|
Load the recommended sample volume per well.
|
Use the Two-Step Loading method.
| ||
Wells damaged during comb removal
|
Remove the comb gently without damaging the wells.
| |
Failure Mode indicated by a steady red light and continuous rapid beeping
|
Defective cassette
|
Disconnect the base and replace gel cassette with a fresh gel cassette.
Press and release the power button to return to Ready Mode.
|
Cold cassette or improper operating conditions
|
Use a cassette stored at room temperature. Avoid storing gel cassettes at 4°C. Use E-Gel® PowerBase™ and E-Gel® Base at room temperature (20-25°C).
| |
Speckles visible
|
Dust fluorescing in same wavelength as SYBR-Safe™
|
Make sure gel is clean before imaging.
|
High background, suboptimal, or no image
|
No filters or wrong filter set
|
Contact the instrument manufacturer for advice.
|
Photographic settings not optimal
|
Optimize settings of your system for E-Gel® with SYBR Safe™ empirically. You may need to increase the exposure time or gain setting.
| |
Stripes visible on image
|
No IR coating on camera when using an UV system
|
Use IR blocking filter or emission filter with IR coating.
|
Filter Selection Guide
Use the filter recommended with your instrument below to photograph E-Gel® with SYBR Safe™. We have shown the most popular instruments; other instruments with an excitation source in the UV range or between 470–530 nm may also be used with the proper filter. Contact your instrument manufacturer for advice.
Instrument (Manufacturer) | Excitation Source | Emission Filter |
AlphaImager (Alpha Innotech)
|
302 nm
|
SYB-500
|
AlphaImager HP (Alpha Innotech)
|
302 nm
|
SYB-500
|
AlphaDigiDoc RT (Alpha Innotech)
|
UV transilluminator
| |
Shroud, Camera Stand (Alpha Innotech)
|
UV transilluminator
|
SYB-100
|
DE500 or DE400 light cabinet 2.17” diam.(Alpha Innotech)
|
UV transilluminator
|
SYB-500
|
DE500 or DE400 light cabinet 2” diam. (Alpha Innotech)
|
UV transilluminator
|
SYB-400
|
VersaDoc Imaging Systems (Bio-Rad)
|
Broadband UV
|
520LP
|
Molecular Imager FX Systems (Bio-Rad)
|
488 nm
|
530 nm BP
|
Gel Doc Systems (Bio-Rad)
|
302 nm
|
520DF30 (#170-8074)
|
Typhoon 9400/9410 (GE Healthcare)
|
488 nm
|
520 BP 40
|
Typhoon 9200/9210/8600/8610 (GE Healthcare)
|
488 nm
|
526 SP
|
FluorImager (GE Healthcare)
|
488 nm
|
530 DF 30
|
Storm (GE Healthcare)
|
Blue (fluorescence mode)
| |
VDS-CL (GE Healthcare)
|
Transmission
|
UV Low
|
Ultracam/Gel Imager (Ultra-Lum)
|
UV
|
Yellow Filter (#990-0804-07)
|
Omega Systems (Ultra-Lum)
|
UV
|
520 nm
|
Polaroid Camera (Polaroid)
|
UV
|
SYBR Safe
™ Photographic Filter (S27100)
|
FOTO/Analyst Express/Investigator/Plus/Luminary (FOTODYNE)
|
UV
|
Fluorescent Green (#60-2034)
|
FOTO/Analyst Minivisionary (FOTODYNE)
|
UV
|
Fluorescent Green (#62-4289)
|
FOTO/Analyst Apprentice (FOTODYNE)
|
UV
|
Fluorescent Green (#62-2535)
|
FOTO/Analyst Luminary (FOTODYNE)
|
UV
|
Fluorescent Green (#60-2056)
|
FCR-10 (Polaroid)
|
UV
|
#3-4218
|
FUJI FLA-3000 (FUJI Film)
|
473 nm
|
520LP
|
BioDocIt/AC1/EC3/BioSpectrum (UVP)
|
302 nm
|
SYBR
® Green (#38-0219-01) or SYBR
® Gold (#38-0221-01)
|
Gel Logic (Kodak)
|
UV
|
535 nm WB50
|
Syngene Instruments (Syngene)
|
UV
|
500–600 nm Shortpass filter
|