Invitrogen

CD45RO Monoclonal Antibody (UCHL1), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

25 References

View all (42) CD45RO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD45RO Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD45RO Antibody (11-0457-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Anti-Human CD45RO FITC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-0457-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596999

Product Specific Information

Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

Applications Reported: The UCHL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Specific transcriptional programs differentiate ICOS from CD28 costimulatory signaling in human Naïve CD4⁺ T cells.

Frontiers in immunology

Gigliotti CL,Boggio E,Favero F,Incarnato D,Santoro C,Oliviero S,Rojo JM,Zucchelli S,Persichetti F,Baldanzi G,Dianzani U,Corà D

11-0457-42 was used in Flow cytometry/Cell sorting to suggest that ICOS and CD28 costimulatory signals play distinct roles during the activation of naive T cells by modulating distinct sets of immunological and immunometabolic genes.

Fri Sep 23 00:00:00 EDT 2022

Longitudinal analysis of subtype C envelope tropism for memory CD4⁺ T cell subsets over the first 3 years of untreated HIV-1 infection.

Retrovirology

Gartner MJ,Gorry PR,Tumpach C,Zhou J,Dantanarayana A,Chang JJ,Angelovich TA,Ellenberg P,Laumaea AE,Nonyane M,Moore PL,Lewin SR,Churchill MJ,Flynn JK,Roche M

Published figure using CD45RO monoclonal antibody (Product # 11-0457-42) in Flow Cytometry

Thu Aug 06 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD45RO monoclonal antibody (Product # 11-0457-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues.

JCI insight

Lowe MM,Boothby I,Clancy S,Ahn RS,Liao W,Nguyen DN,Schumann K,Marson A,Mahuron KM,Kingsbury GA,Liu Z,Munoz Sandoval P,Rodriguez RS,Pauli ML,Taravati K,Arron ST,Neuhaus IM,Harris HW,Kim EA,Shin US,Krummel MF,Daud A,Scharschmidt TC,Rosenblum MD

11-0457 was used in Flow cytometry/Cell sorting to compare healthy skin Tregs with those in peripheral blood, inflamed psoriatic skin, and metastatic melanoma.

Thu Dec 19 00:00:00 EST 2019

FUNDAMANT: an interventional 72-week phase 1 follow-up study of AADvac1, an active immunotherapy against tau protein pathology in Alzheimer's disease.

Alzheimer's research & therapy

Novak P,Schmidt R,Kontsekova E,Kovacech B,Smolek T,Katina S,Fialova L,Prcina M,Parrak V,Dal-Bianco P,Brunner M,Staffen W,Rainer M,Ondrus M,Ropele S,Smisek M,Sivak R,Zilka N,Winblad B,Novak M

11-0457 was used in Flow cytometry/Cell sorting to follow-up on patients following a 24-week phase 1 trial on the active vaccine AADvac1 against pathological tau protein.

Wed Oct 24 00:00:00 EDT 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD45RO monoclonal antibody (Product # 11-0457-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

A different representation of natural T cells and natural killer cells between tumor-infiltrating and periphery lymphocytes in human hepatocellular carcinoma.

Oncology letters

Li XF,Dai D,Song XY,Liu JJ,Zhu L,Zhu X,Ma W,Xu W

11-0457 was used in Flow cytometry/Cell sorting to describe natural T cells and NK cells in local tumour tissues and the periphery blood of patients with human hepatocellular carcinoma.

Mon May 01 00:00:00 EDT 2017

Th2/1 Hybrid Cells Occurring in Murine and Human Strongyloidiasis Share Effector Functions of Th1 Cells.

Frontiers in cellular and infection microbiology

Bock CN,Babu S,Breloer M,Rajamanickam A,Boothra Y,Brunn ML,Kühl AA,Merle R,Löhning M,Hartmann S,Rausch S

11-0457 was used in Flow cytometry/Cell sorting to assess the T helper cell phenotypes in threadworm-infected patients and experimental murine infections with focus on CD4+ T cells co-expressing markers of Th2 and Th1 differentiation.

Mon Feb 26 00:00:00 EST 2018

Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.

PLoS pathogens

Tomić A,Varanasi PR,Golemac M,Malić S,Riese P,Borst EM,Mischak-Weissinger E,Guzmán CA,Krmpotić A,Jonjić S,Messerle M

11-0457 was used in Flow cytometry/Cell sorting to investigate a strategy for an HCMV vaccine that aims at the simultaneous activation of innate and adaptive immune responses.

Thu Dec 01 00:00:00 EST 2016

Durable complete responses off all treatment in patients with metastatic malignant melanoma after sequential immunotherapy followed by a finite course of BRAF inhibitor therapy.

Cancer biology & therapy

Wyluda EJ,Cheng J,Schell TD,Haley JS,Mallon C,Neves RI,Robertson G,Sivik J,Mackley H,Talamo G,Drabick JJ

11-0457 was used in Flow cytometry/Cell sorting to study the effectiveness of different therapy regimes on treating melanoma after failure of immunotherapy.

Tue Mar 01 00:00:00 EST 2016

Do patients with a failed metal-on-metal hip implant with a pseudotumor present differences in their peripheral blood lymphocyte subpopulations?

Clinical orthopaedics and related research

Catelas I,Lehoux EA,Hurda I,Baskey SJ,Gala L,Foster R,Kim PR,Beaulé PE

11-0457 was used in Flow cytometry/Cell sorting to reveal the presence of a type IV hypersensitivity reaction in patients with a failed hip implant with a pseudotumour.

Tue Dec 01 00:00:00 EST 2015

Functional expression of CD137 (4-1BB) on T helper follicular cells.

Oncoimmunology

Alfaro C,Echeveste JI,Rodriguez-Ruiz ME,Solorzano JL,Perez-Gracia JL,Idoate MA,Lopez-Picazo JM,Sanchez-Paulete AR,Labiano S,Rouzaut A,Oñate C,Aznar A,Lozano MD,Melero I

Published figure using CD45RO monoclonal antibody (Product # 11-0457-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Impaired survival of regulatory T cells in pulmonary sarcoidosis.

Respiratory research

Broos CE,van Nimwegen M,Kleinjan A,ten Berge B,Muskens F,in 't Veen JC,Annema JT,Lambrecht BN,Hoogsteden HC,Hendriks RW,Kool M,van den Blink B

11-0457 was used in Flow cytometry/Cell sorting to investigate the role of impaired Treg function in sarcoidosis.

Wed Sep 16 00:00:00 EDT 2015

Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.

PLoS neglected tropical diseases

Katawa G,Layland LE,Debrah AY,von Horn C,Batsa L,Kwarteng A,Arriens S,W Taylor D,Specht S,Hoerauf A,Adjobimey T

11-0457 was used in Flow cytometry/Cell sorting to provide initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating the development of severe onchocerciasis.

Thu Jan 01 00:00:00 EST 2015

Increased Th17 cells in flow cytometer-sorted CD45RO-positive memory CD4 T cells from patients with systemic lupus erythematosus.

Lupus science & medicine

Liu MF,Wang CR

11-0457 was used in Flow cytometry/Cell sorting to show that increased frequencies of Th17 cells positively correlate with disease activity and proteinuria in SLE patients, confirming the dysregulation of Th17/IL-17 in SLE.

Thu Jan 01 00:00:00 EST 2015

Immune responses induced by T-cell vaccination in patients with rheumatoid arthritis.

Human vaccines & immunotherapeutics

Ivanova I,Seledtsova G,Mamaev S,Shishkov A,Seledtsov V

11-0457 was used in Flow cytometry/Cell sorting to study how the T-cell immunotherapy regimen used resulted in the clinical improvement, which was achieved in 87% patients.

Mon Jun 22 00:00:00 EDT 2015

Expression of the memory marker CD45RO on helper T cells in macaques.

PloS one

Valentine M,Song K,Maresh GA,Mack H,Huaman MC,Polacino P,Ho O,Cristillo A,Kyung Chung H,Hu SL,Pincus SH

11-0457 was used in Binding experiments to identify and eliminate subsets of T cells known to house a latent reservoir of HIV.

Tue Apr 08 00:00:00 EDT 2014

Expression of the memory marker CD45RO on helper T cells in macaques.

PloS one

Valentine M,Song K,Maresh GA,Mack H,Huaman MC,Polacino P,Ho O,Cristillo A,Kyung Chung H,Hu SL,Pincus SH

11-0457 was used in Binding experiments to identify and eliminate subsets of T cells known to house a latent reservoir of HIV.

Tue Apr 08 00:00:00 EDT 2014

The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin.

PloS one

Qi Y,Operario DJ,Georas SN,Mosmann TR

11-0457 was used in Flow cytometry/Cell sorting to collate a detailed analysis of the regulation of Amphiregulin expression by human T cell subsets.

Thu Dec 13 00:00:00 EST 2012

Central role of JC virus-specific CD4+ lymphocytes in progressive multi-focal leucoencephalopathy-immune reconstitution inflammatory syndrome.

Brain : a journal of neurology

Aly L,Yousef S,Schippling S,Jelcic I,Breiden P,Matschke J,Schulz R,Bofill-Mas S,Jones L,Demina V,Linnebank M,Ogg G,Girones R,Weber T,Sospedra M,Martin R

11-0457 was used in Flow cytometry/Cell sorting to provide novel insight into the pathogenesis of progressive multi-focal leucoencephalopathy-immune reconstitution inflammatory syndrome.

Thu Sep 01 00:00:00 EDT 2011

Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers.

Proceedings of the National Academy of Sciences of the United States of America

Bentebibel SE,Schmitt N,Banchereau J,Ueno H

11-0457 was used in Immunofluorescence to identify and investigate the role of a previously undefined BCL6-expressing CD4(+) T-cell subset in human tonsils.

Tue Aug 16 00:00:00 EDT 2011

Differential expression of CD300a/c on human TH1 and TH17 cells.

BMC immunology

Simhadri VR,Mariano JL,Zhou Q,DeBell KE,Borrego F

11-0457 was used in Flow cytometry/Cell sorting to conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells, and that their ligation is capable of modulating TCR mediated signals.

Wed Nov 02 00:00:00 EDT 2011

Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.

PloS one

Narayanan S,Silva R,Peruzzi G,Alvarez Y,Simhadri VR,Debell K,Coligan JE,Borrego F

11-0457 was used in Flow cytometry/Cell sorting to investigate a subset within the CD4 T cell population defined by expression of the immunomodulatory receptor CD300a.

Thu May 13 00:00:00 EDT 2010

Frequency of gC1qR+CD4+ T cells increases during acute hepatitis C virus infection and remains elevated in patients with chronic infection.

Clinical immunology (Orlando, Fla.)

Cummings KL,Rosen HR,Hahn YS

11-0457 was used in Flow cytometry/Cell sorting to suggest that HCV infection expands gC1qR+CD4+ T cells, which ultimately facilitates the establishment of HCV persistency.

Tue Sep 01 00:00:00 EDT 2009

The effects of trastuzumab on the CD4+CD25+FoxP3+ and CD4+IL17A+ T-cell axis in patients with breast cancer.

British journal of cancer

Horlock C,Stott B,Dyson PJ,Morishita M,Coombes RC,Savage P,Stebbing J

11-0457 was used in Immunocytochemistry to investigate the therapeutic role of trastuzumab in modulating the activity of the cellular immune system in patients with breast cancer.

Tue Apr 07 00:00:00 EDT 2009

Phenotypical characterization of human Th17 cells unambiguously identified by surface IL-17A expression.

Journal of immunology (Baltimore, Md. : 1950)

Brucklacher-Waldert V,Steinbach K,Lioznov M,Kolster M,Hölscher C,Tolosa E

11-0457 was used in Flow cytometry/Cell sorting to demonstrate a potential method for using IL-17A as a specific marker for human and mouse Th17 cells.

Sun Nov 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45RO Monoclonal Antibody (UCHL1), FITC, eBioscience™

CD45RO Antibody (11-0457-42) in Flow

Product Details

11-0457-42

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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