Invitrogen

CD45RO Monoclonal Antibody (UCHL1), PerCP-eFluor™ 710, eBioscience™

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This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

9 References

View all (42) CD45RO antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

CD45RO Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD45RO Antibody (46-0457-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RA PE (Product # 12-0458-42) and staining buffer (autofluorescence) (left) or Anti-Human CD45RO PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

46-0457-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834405

Product Specific Information

Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

Applications Reported: This UCHL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of normal peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Longitudinal analysis of subtype C envelope tropism for memory CD4⁺ T cell subsets over the first 3 years of untreated HIV-1 infection.

Retrovirology

Gartner MJ,Gorry PR,Tumpach C,Zhou J,Dantanarayana A,Chang JJ,Angelovich TA,Ellenberg P,Laumaea AE,Nonyane M,Moore PL,Lewin SR,Churchill MJ,Flynn JK,Roche M

Published figure using CD45RO monoclonal antibody (Product # 46-0457-42) in Flow Cytometry

Thu Aug 06 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD45RO monoclonal antibody (Product # 46-0457-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Differentiation into an Effector Memory Phenotype Potentiates HIV-1 Latency Reversal in CD4⁺ T Cells.

Journal of virology

Kulpa DA,Talla A,Brehm JH,Ribeiro SP,Yuan S,Bebin-Blackwell AG,Miller M,Barnard R,Deeks SG,Hazuda D,Chomont N,Sékaly RP

46-0457-42 was used in Flow Cytometry to support the idea that the effector memory phenotype supports HIV-1 latency reversal in CD4+ T cells.

Sun Dec 15 00:00:00 EST 2019

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD45RO monoclonal antibody (Product # 46-0457-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Early-life compartmentalization of human T cell differentiation and regulatory function in mucosal and lymphoid tissues.

Nature medicine

Thome JJ,Bickham KL,Ohmura Y,Kubota M,Matsuoka N,Gordon C,Granot T,Griesemer A,Lerner H,Kato T,Farber DL

46-0457 was used in Flow cytometry/Cell sorting to elucidate how the immune system avoids excessive activation as a result of encountering diverse new antigens in early life.

Fri Jan 01 00:00:00 EST 2016

Functional expression of CD137 (4-1BB) on T helper follicular cells.

Oncoimmunology

Alfaro C,Echeveste JI,Rodriguez-Ruiz ME,Solorzano JL,Perez-Gracia JL,Idoate MA,Lopez-Picazo JM,Sanchez-Paulete AR,Labiano S,Rouzaut A,Oñate C,Aznar A,Lozano MD,Melero I

Published figure using CD45RO monoclonal antibody (Product # 46-0457-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Distribution and compartmentalization of human circulating and tissue-resident memory T cell subsets.

Immunity

Sathaliyawala T,Kubota M,Yudanin N,Turner D,Camp P,Thome JJ,Bickham KL,Lerner H,Goldstein M,Sykes M,Kato T,Farber DL

46-0457 was used in Flow cytometry/Cell sorting to perform a multi-dimensional analysis of human T cells in lymphoid and mucosal tissues obtained from individual organ donors.

Thu Jan 24 00:00:00 EST 2013

Association of a NOD2 gene polymorphism and T-helper 17 cells with presumed ocular toxoplasmosis.

The Journal of infectious diseases

Dutra MS,Béla SR,Peixoto-Rangel AL,Fakiola M,Cruz AG,Gazzinelli A,Quites HF,Bahia-Oliveira LM,Peixe RG,Campos WR,Higino-Rocha AC,Miller NE,Blackwell JM,Antonelli LR,Gazzinelli RT

46-0457 was used in Flow cytometry/Cell sorting to suggest that NOD2 influences the production of IL-17A by CD4 T lymphocytes and might contribute to the development of ocular toxoplasmosis.

Tue Jan 01 00:00:00 EST 2013

CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Landrigan A,Wong MT,Utz PJ

46-0457 was used in Flow cytometry/Cell sorting to study the ability of oligodeoxynucleotides to activate and polarise T cells.

Thu Sep 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45RO Monoclonal Antibody (UCHL1), PerCP-eFluor™ 710, eBioscience™

CD45RO Antibody (46-0457-42) in Flow

Product Details

46-0457-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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