Invitrogen

CD45RO Monoclonal Antibody (UCHL1), PE, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

15 References

View all (42) CD45RO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD45RO Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

CD45RO Antibody (12-0457-42) in Flow

Knockout of CD45RO was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR664203_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD45RO was performed by staining THP-1 CD45RO Knock out cells with 0.5 µg Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ (Product # 12-4724-82, yellow histogram) or 0.5 µg CD45RO Monoclonal Antibody (UCHL1), PE, eBioscience™ (Product # 12-0457-42, blue histogram). THP-1 Cas9 control cells were also stained with... View More

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Product Details

12-0457-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272079

Product Specific Information

Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

Applications Reported: The UCHL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Longitudinal analysis of subtype C envelope tropism for memory CD4⁺ T cell subsets over the first 3 years of untreated HIV-1 infection.

Retrovirology

Gartner MJ,Gorry PR,Tumpach C,Zhou J,Dantanarayana A,Chang JJ,Angelovich TA,Ellenberg P,Laumaea AE,Nonyane M,Moore PL,Lewin SR,Churchill MJ,Flynn JK,Roche M

Published figure using CD45RO monoclonal antibody (Product # 12-0457-42) in Flow Cytometry

Thu Aug 06 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD45RO monoclonal antibody (Product # 12-0457-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Clinically compliant spatial and temporal imaging of chimeric antigen receptor T-cells.

Nature communications

Emami-Shahri N,Foster J,Kashani R,Gazinska P,Cook C,Sosabowski J,Maher J,Papa S

12-0457 was used in Flow cytometry/Cell sorting to show the utility of the human sodium iodide symporter (hNIS) for the temporal and spatial monitoring of CAR T-cell behavior in a cancer-bearing host.

Wed Mar 14 00:00:00 EDT 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

12-0457 was used in Flow cytometry/Cell sorting to demonstrate that blocking PITPNM3 recognition of CCL18 could be an attractive immunotherapy aproach for treating breast cancer.

Sat Apr 01 00:00:00 EDT 2017

Modulation of Endoplasmic Reticulum Stress Controls CD4⁺ T-cell Activation and Antitumor Function.

Cancer immunology research

Thaxton JE,Wallace C,Riesenberg B,Zhang Y,Paulos CM,Beeson CC,Liu B,Li Z

12-0457 was used in Flow cytometry/Cell sorting to investigate whether the endoplasmic reticulum stress response may be important for CD4+ T-cell activation and whether gp96 is integral to this process.

Tue Aug 01 00:00:00 EDT 2017

Effector and Central Memory Poly-Functional CD4(+) and CD8(+) T Cells are Boosted upon ZOSTAVAX(®) Vaccination.

Frontiers in immunology

Sei JJ,Cox KS,Dubey SA,Antonello JM,Krah DL,Casimiro DR,Vora KA

12-0457 was used in Flow cytometry to characterise the breadth, magnitude, and quality of ex vivo CD4(+) and CD8(+) T cell responses induced 3-4 weeks after ZOSTAVAX vaccination of healthy adults.

Fri Nov 20 00:00:00 EST 2015

Functional expression of CD137 (4-1BB) on T helper follicular cells.

Oncoimmunology

Alfaro C,Echeveste JI,Rodriguez-Ruiz ME,Solorzano JL,Perez-Gracia JL,Idoate MA,Lopez-Picazo JM,Sanchez-Paulete AR,Labiano S,Rouzaut A,Oñate C,Aznar A,Lozano MD,Melero I

12-0457 was used in Flow cytometry/Cell sorting to ascertain which cell subsets and histological compartments of secondary lymphoid organs express CD137 in healthy conditions or under naturally occurring inflammation.

Tue Dec 01 00:00:00 EST 2015

Somatic Variation of T-Cell Receptor Genes Strongly Associate with HLA Class Restriction.

PloS one

Klarenbeek PL,Doorenspleet ME,Esveldt RE,van Schaik BD,Lardy N,van Kampen AH,Tak PP,Plenge RM,Baas F,de Bakker PI,de Vries N

12-0457 was used in Flow cytometry/Cell sorting to investigate how a single T cell commits to become either CD4+ or CD8+.

Wed Jun 08 00:00:00 EDT 2016

Delta-like 1-mediated Notch signaling enhances the in vitro conversion of human memory CD4 T cells into FOXP3-expressing regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Mota C,Nunes-Silva V,Pires AR,Matoso P,Victorino RM,Sousa AE,Caramalho I

12-0457 was used in Flow cytometry/Cell sorting to investigate novel strategies to generate in vitro-induced Tregs.

Mon Dec 15 00:00:00 EST 2014

Signal transducer and activator of transcription 3 (STAT3) mutations underlying autosomal dominant hyper-IgE syndrome impair human CD8(+) T-cell memory formation and function.

The Journal of allergy and clinical immunology

Ives ML,Ma CS,Palendira U,Chan A,Bustamante J,Boisson-Dupuis S,Arkwright PD,Engelhard D,Averbuch D,Magdorf K,Roesler J,Peake J,Wong M,Adelstein S,Choo S,Smart JM,French MA,Fulcher DA,Cook MC,Picard C,Durandy A,Tsumura M,Kobayashi M,Uzel G,Casanova JL,Tangye SG,Deenick EK

12-0457 was used in Flow cytometry/Cell sorting to investigate the role of IL-21 and associated pathways in CD8(+) T-cell immunity in humans, showing that STAT3 mutations impair CD8(+) T-cell memory formation and function.

Thu Aug 01 00:00:00 EDT 2013

Expression of the memory marker CD45RO on helper T cells in macaques.

PloS one

Valentine M,Song K,Maresh GA,Mack H,Huaman MC,Polacino P,Ho O,Cristillo A,Kyung Chung H,Hu SL,Pincus SH

12-0457 was used in Binding experiments to identify and eliminate subsets of T cells known to house a latent reservoir of HIV.

Tue Apr 08 00:00:00 EDT 2014

Expression of the memory marker CD45RO on helper T cells in macaques.

PloS one

Valentine M,Song K,Maresh GA,Mack H,Huaman MC,Polacino P,Ho O,Cristillo A,Kyung Chung H,Hu SL,Pincus SH

12-0457 was used in Binding experiments to identify and eliminate subsets of T cells known to house a latent reservoir of HIV.

Tue Apr 08 00:00:00 EDT 2014

Dual role of miR-21 in CD4+ T-cells: activation-induced miR-21 supports survival of memory T-cells and regulates CCR7 expression in naive T-cells.

PloS one

Smigielska-Czepiel K,van den Berg A,Jellema P,Slezak-Prochazka I,Maat H,van den Bos H,van der Lei RJ,Kluiver J,Brouwer E,Boots AM,Kroesen BJ

12-0457 was used in Flow cytometry/Cell sorting to investigate the contribution of miR-21 to immune cell function.

Mon Jul 28 00:00:00 EDT 2014

Longevity and composition of cellular immune responses following experimental Plasmodium falciparum malaria infection in humans.

PLoS pathogens

Teirlinck AC,McCall MB,Roestenberg M,Scholzen A,Woestenenk R,de Mast Q,van der Ven AJ,Hermsen CC,Luty AJ,Sauerwein RW

12-0457 was used in Flow cytometry/Cell sorting to study cellular immunological responses to sporozoites and asexual blood-stage malaria parasites.

Thu Dec 01 00:00:00 EST 2011

CpG and non-CpG oligodeoxynucleotides directly costimulate mouse and human CD4+ T cells through a TLR9- and MyD88-independent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Landrigan A,Wong MT,Utz PJ

12-0457 was used in Flow cytometry/Cell sorting to study the ability of oligodeoxynucleotides to activate and polarise T cells.

Thu Sep 15 00:00:00 EDT 2011

Impaired CD4 and CD8 effector function and decreased memory T cell populations in ICOS-deficient patients.

Journal of immunology (Baltimore, Md. : 1950)

Takahashi N,Matsumoto K,Saito H,Nanki T,Miyasaka N,Kobata T,Azuma M,Lee SK,Mizutani S,Morio T

12-0457 was used in Flow cytometry/Cell sorting to investigate the effect of ICOS deficiency on T cell immune responses, showing that it leads to impaired CD4 and CD8 effector function and decreased memory T cell populations.

Fri May 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45RO Monoclonal Antibody (UCHL1), PE, eBioscience™

CD45RO Antibody (12-0457-42) in Flow

Product Details

12-0457-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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