Invitrogen

CD56 (NCAM) Monoclonal Antibody (TULY56), FITC, eBioscience™

CD56 (NCAM) Antibody in Flow Cytometry (Flow)

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CD56 (NCAM) Antibody (11-0566-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 PerCP-eFluor® 710 (Product # 46-0036-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD56 (NCAM) FITC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-0566-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human, Non-human primate, Rhesus monkey

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TULY56

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572459

Product Specific Information

Description: This TULY56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells.

Staining with TULY56 does not block binding of CMSSB, suggesting that the two antibodies recognize different epitopes. Additionally, TULY56 performs better after fixation and permeabilization than CMSSB.

The TULY56 monoclonal antibody crossreacts with Rhesus macaque.

Applications Reported: This TULY56 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TULY56 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Single-cell sequencing of ascites fluid illustrates heterogeneity and therapy-induced evolution during gastric cancer peritoneal metastasis.

Nature communications

Huang XZ,Pang MJ,Li JY,Chen HY,Sun JX,Song YX,Ni HJ,Ye SY,Bai S,Li TH,Wang XY,Lu JY,Yang JJ,Sun X,Mills JC,Miao ZF,Wang ZN

11-0566-42 was used in Flow cytometry/Cell sorting to describe different cell developmental lineage trajectories in the peritoneal ecosystem and provide evidence to better understand the mechanism of GCPM, aiding exploration of effective target strategies for GCPM therapy.

Tue Feb 14 00:00:00 EST 2023

HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway.

Journal of experimental & clinical cancer research : CR

Liu Y,Xu Q,Deng F,Zheng Z,Luo J,Wang P,Zhou J,Lu X,Zhang L,Chen Z,Zhang Q,Chen Q,Zuo D

11-0566-42 was used in Flow cytometry/Cell sorting to identify HERC2 functions as a previously unknown modulator of the JAK2/STAT3 pathway, thereby promoting inflammation-induced stemness and immune evasion in HCC.

Wed Feb 01 00:00:00 EST 2023

CD39/CD73 Dysregulation of Adenosine Metabolism Increases Decidual Natural Killer Cell Cytotoxicity: Implications in Unexplained Recurrent Spontaneous Abortion.

Frontiers in immunology

Zhu J,Song G,Zhou X,Han TL,Yu X,Chen H,Mansell T,Novakovic B,Baker PN,Cannon RD,Saffery R,Chen C,Zhang H

11-0566-42 was used in Flow cytometry/Cell sorting to investigate the regulation of decidual natural killer (dNK) cells and fetal extravillous trophoblast (EVT) cells by CD39 and CD73 in URSA, as well as the possible regulatory mechanism of CD39/CD73 via the TGF-β-mTOR-HIF-1α pathway using clinical samples and cell models.

Fri Apr 08 00:00:00 EDT 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

11-0566-42 was used in Flow Cytometry to pave the way for LSC-directed disease monitoring and therapy in this disease.

Mon Feb 01 00:00:00 EST 2021

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

11-0566-42 was used in Flow Cytometry to pave the way for LSC-directed disease monitoring and therapy in this disease.

Mon Feb 01 00:00:00 EST 2021

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

Published figure using CD56 (NCAM) monoclonal antibody (Product # 11-0566-42) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

11-0566-42 was used in Flow Cytometry to characterize the phenotypic profiles of SARS-CoV-2 antigen-reactive T cells.

Mon Jul 26 00:00:00 EDT 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

11-0566-42 was used in Flow Cytometry to characterize the phenotypic profiles of SARS-CoV-2 antigen-reactive T cells.

Mon Jul 26 00:00:00 EDT 2021

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

11-0566 was used in Flow cytometry/Cell sorting to study the HDAC inhibitor CG-745 and its function to enable the synergistic effects of the immune checkpoint inhibitor combination therapy in various cancers by suppressing tumor microenvironment.

Mon Sep 28 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Mon Sep 28 00:00:00 EDT 2020

Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators.

Stem cell reports

Kwon JB,Vankara A,Ettyreddy AR,Bohning JD,Gersbach CA

11-0566 was used in Flow cytometry/Cell sorting to demonstrate that endogenous activation of the PAX7 transcription factor results in stable epigenetic remodeling and differentiates human pluripotent stem cells into skeletal myogenic progenitor cells.

Tue May 12 00:00:00 EDT 2020

Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators.

Stem cell reports

Kwon JB,Vankara A,Ettyreddy AR,Bohning JD,Gersbach CA

Tue May 12 00:00:00 EDT 2020

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD56 (NCAM) monoclonal antibody (Product # 11-0566-42) in Flow Cytometry

Mon Sep 02 00:00:00 EDT 2019

Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.

Nature communications

Zou F,Lu L,Liu J,Xia B,Zhang W,Hu Q,Liu W,Zhang Y,Lin Y,Jing S,Huang M,Huang B,Liu B,Zhang H

Published figure using CD56 (NCAM) monoclonal antibody (Product # 11-0566-42) in Flow Cytometry

Wed Sep 11 00:00:00 EDT 2019

Cytotoxic effects of ex vivo-expanded natural killer cell-enriched lymphocytes (MYJ1633) against liver cancer.

BMC cancer

Choi JW,Lee ES,Kim SY,Park SI,Oh S,Kang JH,Ryu HA,Lee S

11-0566-42 was used in Flow Cytometry to show that the house-developed method can be a new and effective strategy to prepare clinically applicable NKL for autologous NK cell-based anti-tumor immunotherapy.

Mon Aug 19 00:00:00 EDT 2019

Increased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers.

Journal of virology

Walker WE,Kurscheid S,Joshi S,Lopez CA,Goh G,Choi M,Barakat L,Francis J,Fisher A,Kozal M,Zapata H,Shaw A,Lifton R,Sutton RE,Fikrig E

Fri May 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

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Gene Aliases: CD56; MSK39; NCAM; NCAM1

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UniProt ID: (Human) P13591

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Entrez Gene ID: (Human) 4684, (Rhesus monkey) 693789

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Function(s)

virus receptor activity Ras guanyl-nucleotide exchange factor activity

Process(es)

MAPK cascade cell adhesion axon guidance positive regulation of GTPase activity viral entry into host cell regulation of synaptic plasticity interferon-gamma-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD56 (NCAM) Monoclonal Antibody (TULY56), FITC, eBioscience™

CD56 (NCAM) Antibody (11-0566-42) in Flow

Product Details

11-0566-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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