Invitrogen

CD56 (NCAM) Monoclonal Antibody (TULY56), APC, eBioscience™

CD56 (NCAM) Antibody in Flow Cytometry (Flow)

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CD56 (NCAM) Antibody (17-0566-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 PE (Product # 12-0037-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD56 (NCAM) APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-0566-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 15 publications 15 publications

Product Specifications

Species Reactivity

Human, Non-human primate, Rhesus monkey

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TULY56

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573148

Product Specific Information

Description: This TULY56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells.

Staining with TULY56 does not block binding of CMSSB, suggesting that the two antibodies recognize different epitopes. Additionally, TULY56 performs better after fixation and permeabilization than CMSSB.

The TULY56 monoclonal antibody crossreacts with Rhesus macaque.

Applications Reported: This TULY56 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TULY56 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Exhaustion‑like dysfunction of T and NKT cells in an X‑linked severe combined immunodeficiency patient with maternal engraftment by single‑cell analysis.

International journal of molecular medicine

Dong W,Li W,Zhang S,Zeng X,Qin Q,Fan H,Tang Z,Wu X,Lu G

17-0566-42 was used in Flow cytometry/Cell sorting to report an X-SCID patient with a de novo c.677C>T (p.R226H) variant in exon 5 of IL2RG, exhibiting recurrent and persistent infections from 3-months-old.

Wed Mar 01 00:00:00 EST 2023

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD56 (NCAM) monoclonal antibody (Product # 17-0566-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus.

Journal of immunology research

Satria RD,Huang TW,Jhan MK,Shen TJ,Tseng PC,Wang YT,Yang ZY,Hsing CH,Lin CF

Published figure using CD56 (NCAM) monoclonal antibody (Product # 17-0566-42) in Flow Cytometry

Mon Dec 06 00:00:00 EST 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD56 (NCAM) monoclonal antibody (Product # 17-0566-42) in Flow Cytometry

Mon Jul 26 00:00:00 EDT 2021

Immune cell profiling of the cerebrospinal fluid enables the characterization of the brain metastasis microenvironment.

Nature communications

Rubio-Perez C,Planas-Rigol E,Trincado JL,Bonfill-Teixidor E,Arias A,Marchese D,Moutinho C,Serna G,Pedrosa L,Iurlaro R,Martínez-Ricarte F,Escudero L,Cordero E,Cicuendez M,Ruiz S,Parra G,Nuciforo P,Gonzalez J,Pineda E,Sahuquillo J,Tabernero J,Heyn H,Seoane J

17-0566 was used in Flow cytometry/Cell sorting to characterize immune cells present in brain lesions and matched cerebrospinal fluid (CSF) using single-cell RNA sequencing combined with T cell receptor genotyping.

Mon Mar 08 00:00:00 EST 2021

NKG2C^pos NK Cells Regulate the Expansion of Cytomegalovirus-Specific CD8 T Cells.

Journal of immunology (Baltimore, Md. : 1950)

Grutza R,Moskorz W,Senff T,Bäcker E,Lindemann M,Zimmermann A,Uhrberg M,Lang PA,Timm J,Cosmovici C

17-0566-42 was used in Flow Cytometry to show that NKG2Cpos NK cells interact with HLA-Ehigh CD8 T cells, which may negatively regulate the expansion of CMV-specific CD8 T cells upon activation.

Mon Jun 01 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD56 (NCAM) monoclonal antibody (Product # 17-0566-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators.

Stem cell reports

Kwon JB,Vankara A,Ettyreddy AR,Bohning JD,Gersbach CA

Published figure using CD56 (NCAM) monoclonal antibody (Product # 17-0566-42) in Flow Cytometry

Tue May 12 00:00:00 EDT 2020

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD56 (NCAM) monoclonal antibody (Product # 17-0566-42) in Flow Cytometry

Mon Sep 02 00:00:00 EDT 2019

Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.

Nature communications

Zou F,Lu L,Liu J,Xia B,Zhang W,Hu Q,Liu W,Zhang Y,Lin Y,Jing S,Huang M,Huang B,Liu B,Zhang H

Published figure using CD56 (NCAM) monoclonal antibody (Product # 17-0566-42) in Flow Cytometry

Wed Sep 11 00:00:00 EDT 2019

DKK2 imparts tumor immunity evasion through β-catenin-independent suppression of cytotoxic immune-cell activation.

Nature medicine

Xiao Q,Wu J,Wang WJ,Chen S,Zheng Y,Yu X,Meeth K,Sahraei M,Bothwell ALM,Chen L,Bosenberg M,Chen J,Sexl V,Sun L,Li L,Tang W,Wu D

17-0566 was used in Flow cytometry/Cell sorting to identify a novel tumour immune-suppressive mechanism and immunotherapeutic targets particularly relevant for colorectal cancers and a subset of melanomas.

Thu Mar 01 00:00:00 EST 2018

Transcriptomal signatures of vaccine adjuvants and accessory immunostimulation of sentinel cells by toll-like receptor 2/6 agonists.

Human vaccines & immunotherapeutics

Salyer ACD,David SA

17-0566 was used in Flow cytometry/Cell sorting to identify transcriptomal signatures of innate immune stimulating molecules.

Tue Jul 03 00:00:00 EDT 2018

Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets.

Journal of thrombosis and haemostasis : JTH

Yu Y,Gool E,Berckmans RJ,Coumans FAW,Barendrecht AD,Maas C,van der Wel NN,Altevogt P,Sturk A,Nieuwland R

17-0566 was used in Flow cytometry/Cell sorting to demonstrate that extracellular vesicles from human saliva facilitate homeostasis at sites of skin injury through exposure of coagulants TF and CD24.

Fri Jun 01 00:00:00 EDT 2018

The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets.

Scandinavian journal of immunology

Albayati Z,Alyami A,Alomar S,Middleton D,Bonnett L,Aleem S,Flanagan BF,Christmas SE

17-0566-42 was used in Flow cytometry/Cell sorting to show that CMV influences HLA-G expression in healthy subjects and may contribute to viral immune evasion.

Wed Nov 01 00:00:00 EDT 2017

Increased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers.

Journal of virology

Walker WE,Kurscheid S,Joshi S,Lopez CA,Goh G,Choi M,Barakat L,Francis J,Fisher A,Kozal M,Zapata H,Shaw A,Lifton R,Sutton RE,Fikrig E

Fri May 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

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Gene Aliases: CD56; MSK39; NCAM; NCAM1

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UniProt ID: (Human) P13591

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Entrez Gene ID: (Human) 4684, (Rhesus monkey) 693789

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Function(s)

virus receptor activity Ras guanyl-nucleotide exchange factor activity

Process(es)

MAPK cascade cell adhesion axon guidance positive regulation of GTPase activity viral entry into host cell regulation of synaptic plasticity interferon-gamma-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD56 (NCAM) Monoclonal Antibody (TULY56), APC, eBioscience™

CD56 (NCAM) Antibody (17-0566-42) in Flow

Product Details

17-0566-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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