Invitrogen

CD57 Monoclonal Antibody (TB01 (TBO1)), FITC, eBioscience™

5 Published Figures

11 References

View all (39) CD57 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

FIGURE: 1 / 6

CD57 Antibody (11-0577-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) APC (Product # 17-0567-42) and Mouse IgM Isotype Control FITC (Product # 11-4752-80) (left) or Anti-Human CD57 FITC (right). Cells in the lymphocyte gate were used for analysis.

CD57 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

CD57 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

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Product Details

11-0577-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM

Recommended Isotype Control

Mouse IgM Isotype Control (11E10), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TB01 (TBO1)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1311193

Product Specific Information

Description: This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells.

Applications Reported: The TB01 (TBO1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TB01 (TBO1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD57, also known as HNK-1 and Leu-7, is an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids, and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is expressed on a subset of natural killer (NK) cells and NK T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Aberrant newborn T cell and microbiota developmental trajectories predict respiratory compromise during infancy.

iScience

McDavid A,Laniewski N,Grier A,Gill AL,Kessler HA,Huyck H,Carbonell E,Holden-Wiltse J,Bandyopadhyay S,Carnahan J,Dylag AM,Topham DJ,Falsey AR,Caserta MT,Pryhuber GS,Gill SR,Scheible KM

Published figure using CD57 monoclonal antibody (Product # 11-0577-42) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

Phenotypic analysis of the unstimulated in vivo HIV CD4 T cell reservoir.

eLife

Neidleman J,Luo X,Frouard J,Xie G,Hsiao F,Ma T,Morcilla V,Lee A,Telwatte S,Thomas R,Tamaki W,Wheeler B,Hoh R,Somsouk M,Vohra P,Milush J,James KS,Archin NM,Hunt PW,Deeks SG,Yukl SA,Palmer S,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 11-0577-42) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Comprehensive Cell Surface Antigen Analysis Identifies Transferrin Receptor Protein-1 (CD71) as a Negative Selection Marker for Human Neuronal Cells.

Stem cells (Dayton, Ohio)

Menon V,Thomas R,Elgueta C,Horl M,Osborn T,Hallett PJ,Bartos M,Isacson O,Pruszak J

Tue Oct 01 00:00:00 EDT 2019

Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells.

Cell reports

Cavrois M,Banerjee T,Mukherjee G,Raman N,Hussien R,Rodriguez BA,Vasquez J,Spitzer MH,Lazarus NH,Jones JJ,Ochsenbauer C,McCune JM,Butcher EC,Arvin AM,Sen N,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 11-0577-42) in Flow Cytometry

Tue Jul 25 00:00:00 EDT 2017

Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells.

Cell reports

Cavrois M,Banerjee T,Mukherjee G,Raman N,Hussien R,Rodriguez BA,Vasquez J,Spitzer MH,Lazarus NH,Jones JJ,Ochsenbauer C,McCune JM,Butcher EC,Arvin AM,Sen N,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 11-0577-42) in Flow Cytometry

Tue Jul 25 00:00:00 EDT 2017

Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand.

PLoS pathogens

Tomić A,Varanasi PR,Golemac M,Malić S,Riese P,Borst EM,Mischak-Weissinger E,Guzmán CA,Krmpotić A,Jonjić S,Messerle M

11-0577 was used in Flow cytometry/Cell sorting to investigate a strategy for an HCMV vaccine that aims at the simultaneous activation of innate and adaptive immune responses.

Thu Dec 01 00:00:00 EST 2016

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

11-0577 was used in Flow cytometry/Cell sorting to demonstrate a method to measure autophagy levels in certain diseases, whilst determining the contribution of autophagy to the efficacy of drugs.

Sun Apr 01 00:00:00 EDT 2012

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

11-0577 was used in Flow cytometry/Cell sorting to demonstrate a method to measure autophagy levels in certain diseases, whilst determining the contribution of autophagy to the efficacy of drugs.

Sun Apr 01 00:00:00 EDT 2012

Immunosenescent CD57+CD4+ T-cells accumulate and contribute to interferon-γ responses in HIV patients responding stably to ART.

Disease markers

Fernandez S,French MA,Price P

11-0577 was used in Flow cytometry/Cell sorting to determine the key factors influencing the pathogenesis of secondary viral infections in HIV patients receiving ART.

Thu May 31 00:00:00 EDT 2012

Common gamma chain cytokines promote rapid in vitro expansion of allo-specific human CD8+ suppressor T cells.

PloS one

Yu Y,Zitzner JR,Houlihan J,Herrera N,Xu L,Miller J,Mathew JM,Tambur AR,Luo X

11-0577 was used in Flow cytometry/Cell sorting to demonstrate the use of common γc cytokines in combination with donor APCs as a strategy for generation of donor-specific T cells for immunotherapy.

Mon Aug 27 00:00:00 EDT 2012

Identification of B cells through negative gating-An example of the MIFlowCyt standard applied.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Blimkie D,Fortuno ES,Thommai F,Xu L,Fernandes E,Crabtree J,Rein-Weston A,Jansen K,Brinkman RR,Kollmann TR

11-0577 was used in Flow cytometry/Cell sorting to demonstrate how a MIFlowCyt-compliant report can be prepared with minimal effort, and yet provide the reader with a much clearer picture of the portrayed FCM experiment and data.

Tue Jun 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Beta-1,3-glucuronyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT-P; GlcUAT-P; Glucuronosyltransferase P; HNK-1; Leu-7; LEU7 antigen; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

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Gene Aliases: B3GAT1; CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1

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UniProt ID: (Human) Q9P2W7

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Entrez Gene ID: (Human) 27087

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Function(s)

galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity metal ion binding glycosyltransferase

Process(es)

carbohydrate metabolic process protein glycosylation glycosaminoglycan metabolic process chondroitin sulfate metabolic process chondroitin sulfate proteoglycan biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD57 Monoclonal Antibody (TB01 (TBO1)), FITC, eBioscience™

CD57 Antibody (11-0577-42) in Flow

Product Details

11-0577-42

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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