Invitrogen

CD57 Monoclonal Antibody (TB01 (TBO1)), eBioscience™

5 Published Figures

8 References

View all (39) CD57 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

CD57 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 6

CD57 Antibody (14-0577-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil stained with 1 µg/mL of Mouse IgM Isotype Control Purified (left) or 1 µg/mL of Anti-Human CD57 Purified (right), followed by Anti-Mouse IgG Biotin, Avidin HRP, and visualized with the DAB Advanced Chromogenic Kit. Nuclei are counterstained with hematoxylin.

CD57 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD57 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

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Product Details

14-0577-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

TB01 (TBO1)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572855

Product Specific Information

Description: This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells.

Applications Reported: This TB01 (TBO1) antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.

Applications Tested: This TB01 (TBO1) antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval. The antibody can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD57, also known as HNK-1 and Leu-7, is an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids, and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is expressed on a subset of natural killer (NK) cells and NK T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aberrant newborn T cell and microbiota developmental trajectories predict respiratory compromise during infancy.

iScience

McDavid A,Laniewski N,Grier A,Gill AL,Kessler HA,Huyck H,Carbonell E,Holden-Wiltse J,Bandyopadhyay S,Carnahan J,Dylag AM,Topham DJ,Falsey AR,Caserta MT,Pryhuber GS,Gill SR,Scheible KM

Published figure using CD57 monoclonal antibody (Product # 14-0577-82) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

Phenotypic analysis of the unstimulated in vivo HIV CD4 T cell reservoir.

eLife

Neidleman J,Luo X,Frouard J,Xie G,Hsiao F,Ma T,Morcilla V,Lee A,Telwatte S,Thomas R,Tamaki W,Wheeler B,Hoh R,Somsouk M,Vohra P,Milush J,James KS,Archin NM,Hunt PW,Deeks SG,Yukl SA,Palmer S,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 14-0577-82) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Human lung natural killer cells are predominantly comprised of highly differentiated hypofunctional CD69^-CD56^dim cells.

The Journal of allergy and clinical immunology

Marquardt N,Kekäläinen E,Chen P,Kvedaraite E,Wilson JN,Ivarsson MA,Mjösberg J,Berglin L,Säfholm J,Manson ML,Adner M,Al-Ameri M,Bergman P,Orre AC,Svensson M,Dahlén B,Dahlén SE,Ljunggren HG,Michaëlsson J

14-0577 was used in Flow cytometry/Cell sorting to analyse and compare the phenotypic and functional characteristics of natural killer cells in the human lung and peripheral blood at the single-cell level.

Sat Apr 01 00:00:00 EDT 2017

Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells.

Cell reports

Cavrois M,Banerjee T,Mukherjee G,Raman N,Hussien R,Rodriguez BA,Vasquez J,Spitzer MH,Lazarus NH,Jones JJ,Ochsenbauer C,McCune JM,Butcher EC,Arvin AM,Sen N,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 14-0577-82) in Flow Cytometry

Tue Jul 25 00:00:00 EDT 2017

Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells.

Cell reports

Cavrois M,Banerjee T,Mukherjee G,Raman N,Hussien R,Rodriguez BA,Vasquez J,Spitzer MH,Lazarus NH,Jones JJ,Ochsenbauer C,McCune JM,Butcher EC,Arvin AM,Sen N,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 14-0577-82) in Flow Cytometry

Tue Jul 25 00:00:00 EDT 2017

Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Marquardt N,Béziat V,Nyström S,Hengst J,Ivarsson MA,Kekäläinen E,Johansson H,Mjösberg J,Westgren M,Lankisch TO,Wedemeyer H,Ellis EC,Ljunggren HG,Michaëlsson J,Björkström NK

14-0577 was used in Flow cytometry/Cell sorting to study the unique lineage of CD49a(+)DX5(-) NK cells, showing that they are readily detectable in the liver but not in hepatic venous or peripheral blood.

Sun Mar 15 00:00:00 EDT 2015

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD57 monoclonal antibody (Product # 14-0577-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD57 monoclonal antibody (Product # 14-0577-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Beta-1,3-glucuronyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT-P; GlcUAT-P; Glucuronosyltransferase P; HNK-1; Leu-7; LEU7 antigen; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

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Gene Aliases: B3GAT1; CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1

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UniProt ID: (Human) Q9P2W7

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Entrez Gene ID: (Human) 27087

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Function(s)

galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity metal ion binding glycosyltransferase

Process(es)

carbohydrate metabolic process protein glycosylation glycosaminoglycan metabolic process chondroitin sulfate metabolic process chondroitin sulfate proteoglycan biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD57 Monoclonal Antibody (TB01 (TBO1)), eBioscience™

CD57 Antibody (14-0577-82) in IHC (P)

Product Details

14-0577-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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