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Invitrogen
CD57 Monoclonal Antibody (TB01 (TBO1)), eFluor™ 660, eBioscience™
FIGURE: 1 / 6
CD57 Antibody (50-0577-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD4 PE-Cyanine7 (Product # 25-0047-42) and Mouse IgM Isotype Control eFluor® 660 (Product # 50-4752-82) (left) or Anti-Human CD57 eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
50-0577-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgM
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TB01 (TBO1)
Conjugate
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2574163
Product Specific Information
Description: This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells.
Applications Reported: This TB01 (TBO1) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TB01 (TBO1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD57, also known as HNK-1 and Leu-7, is an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids, and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is expressed on a subset of natural killer (NK) cells and NK T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: Beta-1,3-glucuronyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT-P; GlcUAT-P; Glucuronosyltransferase P; HNK-1; Leu-7; LEU7 antigen; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
Gene Aliases: B3GAT1; CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1
UniProt ID: (Human) Q9P2W7
Entrez Gene ID: (Human) 27087
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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