Invitrogen

EOMES Monoclonal Antibody (WD1928), FITC, eBioscience™

FIGURE: 1 / 18

EOMES Antibody (11-4877-42) in Flow

Intracellular staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0037-42) (left), Anti-Human CD56 (NCAM) PE (Product # 12-0567-42) (right), and Anti-Human Eomes FITC using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.

EOMES Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

11-4877-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Human, Pig

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

WD1928

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572499

Product Specific Information

Description: This WD1928 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This WD1928 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This WD1928 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A single-cell map of dynamic chromatin landscapes of immune cells in renal cell carcinoma.

Nature cancer

Kourtis N,Wang Q,Wang B,Oswald E,Adler C,Cherravuru S,Malahias E,Zhang L,Golubov J,Wei Q,Lemus S,Ni M,Ding Y,Wei Y,Atwal GS,Thurston G,Macdonald LE,Murphy AJ,Dhanik A,Sleeman MA,Tykodi SS,Skokos D

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2022

Transcriptional profiling of human Vδ1 T cells reveals a pathogen-driven adaptive differentiation program.

Cell reports

McMurray JL,von Borstel A,Taher TE,Syrimi E,Taylor GS,Sharif M,Rossjohn J,Remmerswaal EBM,Bemelman FJ,Vieira Braga FA,Chen X,Teichmann SA,Mohammed F,Berry AA,Lyke KE,Williamson KC,Stubbington MJT,Davey MS,Willcox CR,Willcox BE

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Tue May 24 00:00:00 EDT 2022

TBC1D3 promotes neural progenitor proliferation by suppressing the histone methyltransferase G9a.

Science advances

Hou QQ,Xiao Q,Sun XY,Ju XC,Luo ZG

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Immunohistochemistry

Fri Jan 01 00:00:00 EST 2021

Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells.

STAR protocols

Sia WR,Hey YY,Foo R,Wang LF,Leeansyah E

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Norovirus-Specific CD8⁺ T Cell Responses in Human Blood and Tissues.

Cellular and molecular gastroenterology and hepatology

Pattekar A,Mayer LS,Lau CW,Liu C,Palko O,Bewtra M,Consortium H,Lindesmith LC,Brewer-Jensen PD,Baric RS,Betts MR,Naji A,Wherry EJ,Tomov VT

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2022

Homeostatic Function and Inflammatory Activation of Ileal CD8⁺ Tissue-Resident T Cells Is Dependent on Mucosal Location.

Cellular and molecular gastroenterology and hepatology

Lutter L,Roosenboom B,Brand EC,Ter Linde JJ,Oldenburg B,van Lochem EG,Horjus Talabur Horje CS,van Wijk F

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Mon Mar 28 00:00:00 EDT 2022

Early IFN-α signatures and persistent dysfunction are distinguishing features of NK cells in severe COVID-19.

Immunity

Krämer B,Knoll R,Bonaguro L,ToVinh M,Raabe J,Astaburuaga-García R,Schulte-Schrepping J,Kaiser KM,Rieke GJ,Bischoff J,Monin MB,Hoffmeister C,Schlabe S,De Domenico E,Reusch N,Händler K,Reynolds G,Blüthgen N,Hack G,Finnemann C,Nischalke HD,Strassburg CP,Stephenson E,Su Y,Gardner L,Yuan D,Chen D,Goldman J,Rosenstiel P,Schmidt SV,Latz E,Hrusovsky K,Ball AJ,Johnson JM,Koenig PA,Schmidt FI,Haniffa M,Heath JR,Kümmerer BM,Keitel V,Jensen B,Stubbemann P,Kurth F,Sander LE,Sawitzki B,Aschenbrenner AC,Schultze JL,Nattermann J

11-4877-42 was used in Flow Cytometry /test) to perform characterization of natural killer (NK) cells in 205 patients (403 samples; days 2 to 41 after symptom onset) from four independent cohorts using single-cell transcriptomics and proteomics together with functional studies.

Tue Nov 09 00:00:00 EST 2021

Cell-type-specific 3D epigenomes in the developing human cortex.

Nature

Song M,Pebworth MP,Yang X,Abnousi A,Fan C,Wen J,Rosen JD,Choudhary MNK,Cui X,Jones IR,Bergenholtz S,Eze UC,Juric I,Li B,Maliskova L,Lee J,Liu W,Pollen AA,Li Y,Wang T,Hu M,Kriegstein AR,Shen Y

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

In Situ Maturation and Tissue Adaptation of Type 2 Innate Lymphoid Cell Progenitors.

Immunity

Zeis P,Lian M,Fan X,Herman JS,Hernandez DC,Gentek R,Elias S,Symowski C,Knöpper K,Peltokangas N,Friedrich C,Doucet-Ladeveze R,Kabat AM,Locksley RM,Voehringer D,Bajenoff M,Rudensky AY,Romagnani C,Grün D,Gasteiger G

11-4877-42 was used in Flow Cytometry to identify local ILCPs and reveal ILCP in situ differentiation and tissue adaptation as a mechanism of ILC maintenance and phenotypic diversification.

Tue Oct 13 00:00:00 EDT 2020

A Multiplex Human Pluripotent Stem Cell Platform Defines Molecular and Functional Subclasses of Autism-Related Genes.

Cell stem cell

Cederquist GY,Tchieu J,Callahan SJ,Ramnarine K,Ryan S,Zhang C,Rittenhouse C,Zeltner N,Chung SY,Zhou T,Chen S,Betel D,White RM,Tomishima M,Studer L

11-4877-42 was used in Flow Cytometry to develop a multiplex human pluripotent stem cell platform for the purposes of testing early-developmental hypotheses of autism.

Thu Jul 02 00:00:00 EDT 2020

PD-1 Blockade Reinvigorates Bone Marrow CD8⁺ T Cells from Patients with Multiple Myeloma in the Presence of TGFβ Inhibitors.

Clinical cancer research : an official journal of the American Association for Cancer Research

Kwon M,Kim CG,Lee H,Cho H,Kim Y,Lee EC,Choi SJ,Park J,Seo IH,Bogen B,Song IC,Jo DY,Kim JS,Park SH,Choi I,Choi YS,Shin EC

11-4877-41 was used in Flow Cytometry to indicate that combined blockade of PD-1 and TGFβ may be useful for the treatment of multiple myeloma.

Wed Apr 01 00:00:00 EDT 2020

Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells.

eLife

Bhattacharya P,Ellegård R,Khalid M,Svanberg C,Govender M,Keita ÅV,Söderholm JD,Myrelid P,Shankar EM,Nyström S,Larsson M

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Wed Sep 02 00:00:00 EDT 2020

The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections.

Cell reports

Katsuyama E,Suarez-Fueyo A,Bradley SJ,Mizui M,Marin AV,Mulki L,Krishfield S,Malavasi F,Yoon J,Sui SJH,Kyttaris VC,Tsokos GC

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Tue Jan 07 00:00:00 EST 2020

CD19-CAR-T Cells Bearing a KIR/PD-1-Based Inhibitory CAR Eradicate CD19⁺HLA-C1^- Malignant B Cells While Sparing CD19⁺HLA-C1⁺ Healthy B Cells.

Cancers

Tao L,Farooq MA,Gao Y,Zhang L,Niu C,Ajmal I,Zhou Y,He C,Zhao G,Yao J,Liu M,Jiang W

11-4877 was used in Flow cytometry/Cell sorting to engineer a novel KIR/PD-1-based inhibitory CAR (iKP CAR) by fusing the extracellular domain of killer cell immunoglobulin-like receptors (KIR) 2DL2 (KIR2DL2) and the intracellular domain of PD-1.

Sun Sep 13 00:00:00 EDT 2020

CD19-CAR-T Cells Bearing a KIR/PD-1-Based Inhibitory CAR Eradicate CD19⁺HLA-C1^- Malignant B Cells While Sparing CD19⁺HLA-C1⁺ Healthy B Cells.

Cancers

Tao L,Farooq MA,Gao Y,Zhang L,Niu C,Ajmal I,Zhou Y,He C,Zhao G,Yao J,Liu M,Jiang W

Sun Sep 13 00:00:00 EDT 2020

C reactive protein impairs adaptive immunity in immune cells of patients with melanoma.

Journal for immunotherapy of cancer

Yoshida T,Ichikawa J,Giuroiu I,Laino AS,Hao Y,Krogsgaard M,Vassallo M,Woods DM,Stephen Hodi F,Weber J

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

The TLR9 ligand CpG ODN 2006 is a poor adjuvant for the induction of de novo CD8⁺ T-cell responses in vitro.

Scientific reports

Papagno L,Kuse N,Lissina A,Gostick E,Price DA,Appay V,Nicoli F

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Wed Jul 15 00:00:00 EDT 2020

Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells.

Nature communications

Marquardt N,Kekäläinen E,Chen P,Lourda M,Wilson JN,Scharenberg M,Bergman P,Al-Ameri M,Hård J,Mold JE,Ljunggren HG,Michaëlsson J

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Mon Aug 26 00:00:00 EDT 2019

Tissue-resident Eomes⁺ NK cells are the major innate lymphoid cell population in human infant intestine.

Nature communications

Sagebiel AF,Steinert F,Lunemann S,Körner C,Schreurs RRCE,Altfeld M,Perez D,Reinshagen K,Bunders MJ

Published figure using EOMES monoclonal antibody (Product # 11-4877-42) in Flow Cytometry

Thu Feb 28 00:00:00 EST 2019

Autophagy Regulation of Metabolism Is Required for CD8⁺ T Cell Anti-tumor Immunity.

Cell reports

DeVorkin L,Pavey N,Carleton G,Comber A,Ho C,Lim J,McNamara E,Huang H,Kim P,Zacharias LG,Mizushima N,Saitoh T,Akira S,Beckham W,Lorzadeh A,Moksa M,Cao Q,Murthy A,Hirst M,DeBerardinis RJ,Lum JJ

11-4877 was used in Flow cytometry/Cell sorting to demonstrate a cell-autonomous role for autophagy in the regulation of CD8+ T-cell-mediated control of tumors.

Tue Apr 09 00:00:00 EDT 2019

MAIT cells are activated in acute Dengue virus infection and after in vitro Zika virus infection.

PLoS neglected tropical diseases

Paquin-Proulx D,Avelino-Silva VI,Santos BAN,Silveira Barsotti N,Siroma F,Fernandes Ramos J,Coracini Tonacio A,Song A,Maestri A,Barros Cerqueira N,Felix AC,Levi JE,Greenspun BC,de Mulder Rougvie M,Rosenberg MG,Nixon DF,Kallas EG

11-4877 was used in Flow cytometry/Cell sorting to evaluate mucosal-associated invariant T cell frequency, phenotype, and function in samples from subjects with acute and convalescent dengue virus infection.

Mon Jan 01 00:00:00 EST 2018

Natural Killer Cells Promote Fetal Development through the Secretion of Growth-Promoting Factors.

Immunity

Fu B,Zhou Y,Ni X,Tong X,Xu X,Dong Z,Sun R,Tian Z,Wei H

11-4877-42 was used in Flow Cytometry to reveal properties of Natural Killer (NK) cells in promoting fetal growth.

Tue Dec 19 00:00:00 EST 2017

Effect of Cytomegalovirus (CMV) and Ageing on T-Bet and Eomes Expression on T-Cell Subsets.

International journal of molecular sciences

Hassouneh F,Lopez-Sejas N,Campos C,Sanchez-Correa B,Tarazona R,Pera A,Solana R

11-4877 was used in Flow cytometry/Cell sorting to analyse the effect of Cytomegalovirus and ageing on expression of the transcription factors T-bet, Eomes and CD57 on T cell subsets.

Thu Jun 29 00:00:00 EDT 2017

Fetal CD103+ IL-17-Producing Group 3 Innate Lymphoid Cells Represent the Dominant Lymphocyte Subset in Human Amniotic Fluid.

Journal of immunology (Baltimore, Md. : 1950)

Marquardt N,Ivarsson MA,Sundström E,Åkesson E,Martini E,Eidsmo L,Mjösberg J,Friberg D,Kublickas M,Ek S,Tegerstedt G,Seiger Å,Westgren M,Michaëlsson J

11-4877 was used in Flow cytometry/Cell sorting to indicate that CD103+ ILC3s accumulate with gestation in the fetal intestine before egress to the amniotic fluid.

Sat Oct 15 00:00:00 EDT 2016

Arming of MAIT Cell Cytolytic Antimicrobial Activity Is Induced by IL-7 and Defective in HIV-1 Infection.

PLoS pathogens

Leeansyah E,Svärd J,Dias J,Buggert M,Nyström J,Quigley MF,Moll M,Sönnerborg A,Nowak P,Sandberg JK

11-4877 was used in Flow cytometry/Cell sorting to show that IL-7 enhances the sensitivity of MAIT cells and induces the arming of effector functions.

Sat Aug 01 00:00:00 EDT 2015

Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Marquardt N,Béziat V,Nyström S,Hengst J,Ivarsson MA,Kekäläinen E,Johansson H,Mjösberg J,Westgren M,Lankisch TO,Wedemeyer H,Ellis EC,Ljunggren HG,Michaëlsson J,Björkström NK

11-4877 was used in Flow cytometry/Cell sorting to study the unique lineage of CD49a(+)DX5(-) NK cells, showing that they are readily detectable in the liver but not in hepatic venous or peripheral blood.

Sun Mar 15 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2

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Gene Aliases: EOMES; TBR2

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UniProt ID: (Human) O95936

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Entrez Gene ID: (Human) 8320, (Pig) 100622125

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification trophectodermal cell differentiation adaptive immune response CD8-positive, alpha-beta T cell differentiation involved in immune response transcription, DNA-templated brain development cardioblast differentiation stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation

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EOMES Monoclonal Antibody (WD1928), FITC, eBioscience™

EOMES Antibody (11-4877-42) in Flow

Product Details

11-4877-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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