Invitrogen

EOMES Monoclonal Antibody (WD1928), PerCP-eFluor™ 710, eBioscience™

FIGURE: 1 / 16

EOMES Antibody (46-4877-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) (left) and Anti-Human CD56 APC (Product # 17-0567-42) (right) followed by fixation and permeabilization using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00). Cells were then intracellularly stained with Anti-Human Eomes PerCP-eFluor® 710. Cells in the lymphocyte gate were used for analysis.

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Product Details

46-4877-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human, Pig

Published species

Dog, Human, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

WD1928

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573759

Product Specific Information

Description: This WD1928 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This WD1928 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This WD1928 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Phenotypic and functional characterization of pharmacologically expanded Vγ9Vδ2 T cells in pigtail macaques.

iScience

Barber-Axthelm IM,Wragg KM,Esterbauer R,Amarasena TH,Barber-Axthelm VRB,Wheatley AK,Gibbon AM,Kent SJ,Juno JA

46-4877-42 was used in Flow Cytometry to show the tissue distribution and phenotype of in vivo pharmacologically expanded Vγ9Vδ2 T cells can be altered based on the antigen administration route, with implications for tissue trafficking and the clinical efficacy of Vγ9Vδ2 T cell immunotherapeutic.

Fri Mar 17 00:00:00 EDT 2023

A single-cell map of dynamic chromatin landscapes of immune cells in renal cell carcinoma.

Nature cancer

Kourtis N,Wang Q,Wang B,Oswald E,Adler C,Cherravuru S,Malahias E,Zhang L,Golubov J,Wei Q,Lemus S,Ni M,Ding Y,Wei Y,Atwal GS,Thurston G,Macdonald LE,Murphy AJ,Dhanik A,Sleeman MA,Tykodi SS,Skokos D

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2022

Transcriptional profiling of human Vδ1 T cells reveals a pathogen-driven adaptive differentiation program.

Cell reports

McMurray JL,von Borstel A,Taher TE,Syrimi E,Taylor GS,Sharif M,Rossjohn J,Remmerswaal EBM,Bemelman FJ,Vieira Braga FA,Chen X,Teichmann SA,Mohammed F,Berry AA,Lyke KE,Williamson KC,Stubbington MJT,Davey MS,Willcox CR,Willcox BE

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Tue May 24 00:00:00 EDT 2022

Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells.

STAR protocols

Sia WR,Hey YY,Foo R,Wang LF,Leeansyah E

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Norovirus-Specific CD8⁺ T Cell Responses in Human Blood and Tissues.

Cellular and molecular gastroenterology and hepatology

Pattekar A,Mayer LS,Lau CW,Liu C,Palko O,Bewtra M,Consortium H,Lindesmith LC,Brewer-Jensen PD,Baric RS,Betts MR,Naji A,Wherry EJ,Tomov VT

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2022

Homeostatic Function and Inflammatory Activation of Ileal CD8⁺ Tissue-Resident T Cells Is Dependent on Mucosal Location.

Cellular and molecular gastroenterology and hepatology

Lutter L,Roosenboom B,Brand EC,Ter Linde JJ,Oldenburg B,van Lochem EG,Horjus Talabur Horje CS,van Wijk F

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Mon Mar 28 00:00:00 EDT 2022

Cross-Reactivity and Functionality of Approved Human Immune Checkpoint Blockers in Dogs.

Cancers

Pantelyushin S,Ranninger E,Guerrera D,Hutter G,Maake C,Markkanen E,Bettschart-Wolfensberger R,Rohrer Bley C,Läubli H,Vom Berg J

46-4877 was used in Flow cytometry/Cell sorting to assess suitability of seven FDA-approved human ICIs to target CTLA-4 or PD-1/PD-L1 in dogs.

Sat Feb 13 00:00:00 EST 2021

Cell-type-specific 3D epigenomes in the developing human cortex.

Nature

Song M,Pebworth MP,Yang X,Abnousi A,Fan C,Wen J,Rosen JD,Choudhary MNK,Cui X,Jones IR,Bergenholtz S,Eze UC,Juric I,Li B,Maliskova L,Lee J,Liu W,Pollen AA,Li Y,Wang T,Hu M,Kriegstein AR,Shen Y

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

OMIP-065: Dog Immunophenotyping and T-Cell Activity Evaluation with a 14-Color Panel.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Pantelyushin S,Ranninger E,Bettschart-Wolfensberger R,Vom Berg J

Thu Oct 01 00:00:00 EDT 2020

Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells.

eLife

Bhattacharya P,Ellegård R,Khalid M,Svanberg C,Govender M,Keita ÅV,Söderholm JD,Myrelid P,Shankar EM,Nyström S,Larsson M

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Wed Sep 02 00:00:00 EDT 2020

The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections.

Cell reports

Katsuyama E,Suarez-Fueyo A,Bradley SJ,Mizui M,Marin AV,Mulki L,Krishfield S,Malavasi F,Yoon J,Sui SJH,Kyttaris VC,Tsokos GC

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Tue Jan 07 00:00:00 EST 2020

CD19-CAR-T Cells Bearing a KIR/PD-1-Based Inhibitory CAR Eradicate CD19⁺HLA-C1^- Malignant B Cells While Sparing CD19⁺HLA-C1⁺ Healthy B Cells.

Cancers

Tao L,Farooq MA,Gao Y,Zhang L,Niu C,Ajmal I,Zhou Y,He C,Zhao G,Yao J,Liu M,Jiang W

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Sun Sep 13 00:00:00 EDT 2020

C reactive protein impairs adaptive immunity in immune cells of patients with melanoma.

Journal for immunotherapy of cancer

Yoshida T,Ichikawa J,Giuroiu I,Laino AS,Hao Y,Krogsgaard M,Vassallo M,Woods DM,Stephen Hodi F,Weber J

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

The TLR9 ligand CpG ODN 2006 is a poor adjuvant for the induction of de novo CD8⁺ T-cell responses in vitro.

Scientific reports

Papagno L,Kuse N,Lissina A,Gostick E,Price DA,Appay V,Nicoli F

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Wed Jul 15 00:00:00 EDT 2020

Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells.

Nature communications

Marquardt N,Kekäläinen E,Chen P,Lourda M,Wilson JN,Scharenberg M,Bergman P,Al-Ameri M,Hård J,Mold JE,Ljunggren HG,Michaëlsson J

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Mon Aug 26 00:00:00 EDT 2019

Tissue-resident Eomes⁺ NK cells are the major innate lymphoid cell population in human infant intestine.

Nature communications

Sagebiel AF,Steinert F,Lunemann S,Körner C,Schreurs RRCE,Altfeld M,Perez D,Reinshagen K,Bunders MJ

Published figure using EOMES monoclonal antibody (Product # 46-4877-42) in Flow Cytometry

Thu Feb 28 00:00:00 EST 2019

CMV Primes Functional Alternative Signaling in Adaptive Δg NK Cells but Is Subverted by Lentivirus Infection in Rhesus Macaques.

Cell reports

Shah SV,Manickam C,Ram DR,Kroll K,Itell H,Permar SR,Barouch DH,Klatt NR,Reeves RK

46-4877-42 was used in Flow Cytometry to provide a mechanism of virus-dependent alternative signaling that may explain the acquisition of adaptive features by primate NK cells and could be targeted for future vaccine or curative therapies.

Tue Dec 04 00:00:00 EST 2018

Natural Killer Cells Promote Fetal Development through the Secretion of Growth-Promoting Factors.

Immunity

Fu B,Zhou Y,Ni X,Tong X,Xu X,Dong Z,Sun R,Tian Z,Wei H

46-4877-42 was used in Flow Cytometry to reveal properties of Natural Killer (NK) cells in promoting fetal growth.

Tue Dec 19 00:00:00 EST 2017

Bioinformatics

Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2

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Gene Aliases: EOMES; TBR2

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UniProt ID: (Human) O95936

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Entrez Gene ID: (Human) 8320, (Pig) 100622125

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification trophectodermal cell differentiation adaptive immune response CD8-positive, alpha-beta T cell differentiation involved in immune response transcription, DNA-templated brain development cardioblast differentiation stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EOMES Monoclonal Antibody (WD1928), PerCP-eFluor™ 710, eBioscience™

EOMES Antibody (46-4877-42) in Flow

Product Details

46-4877-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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