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Invitrogen
EOMES Monoclonal Antibody (WD1928), Alexa Fluor™ 660, eBioscience™
FIGURE: 1 / 1
EOMES Antibody (606-4877-42) in Flow
Normal human peripheral blood cells were surface-stained with CD56 (NCAM) Monoclonal Antibody, eFluor 450 (Product # 48-0567-42). Cells were then stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with Mouse IgG1 kappa Isotype Control, Alexa Fluor 660 (Product # 606-4714-81) (left) or EOMES Monoclonal Antibody, Alexa Fluor 660 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18). This ... View More
Product Details
606-4877-42
Product Specifications
Species Reactivity
Human,
Pig
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
WD1928
Conjugate
Excitation/Emission Max
663/691 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2896283
Product Specific Information
Description: The WD1928 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.
Applications Reported: This WD1928 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This WD1928 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at Flow Protocols . This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser.
Target Information
TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2
Gene Aliases: EOMES; TBR2
UniProt ID: (Human) O95936
Entrez Gene ID: (Human) 8320, (Pig) 100622125
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II activating transcription factor binding
transcriptional repressor activity, RNA polymerase II transcription factor binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
endoderm formation
mesoderm formation
endodermal cell fate specification
trophectodermal cell differentiation
adaptive immune response
CD8-positive, alpha-beta T cell differentiation involved in immune response
transcription, DNA-templated
brain development
cardioblast differentiation
stem cell population maintenance
olfactory bulb development
cerebral cortex regionalization
cerebral cortex neuron differentiation
interferon-gamma production
skeletal muscle cell differentiation
positive regulation of cell differentiation
regulation of neuron differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cell differentiation involved in embryonic placenta development
mesodermal to mesenchymal transition involved in gastrulation
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