Invitrogen

CD278 (ICOS) Monoclonal Antibody (7E.17G9), FITC, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

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CD278 (ICOS) Antibody (11-9942-82) in Flow

Staining of unstimulated (left) and 3-day Con A-stimulated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (blue histogram) or 0.06 µg of Anti-Mouse CD278 (ICOS) FITC (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 660, were used for analysis.

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Product Details

11-9942-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

7E.17G9

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11218290

Product Specific Information

Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.

Applications Reported: This 7E.17G9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 7E.17G9 antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9942-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

T cell receptor signaling strength establishes the chemotactic properties of effector CD8⁺ T cells that control tissue-residency.

Nature communications

Abdelbary M,Hobbs SJ,Gibbs JS,Yewdell JW,Nolz JC

11-9942-82 was used in Flow cytometry/Cell sorting to show that access to antigen presentation and strength of TCR-signaling required for Blimp1 expression establishes the chemotactic properties of effector CD8+ T cells to promote residency within non-lymphoid tissues.

Tue Jul 04 00:00:00 EDT 2023

Chemotherapy-induced ileal crypt apoptosis and the ileal microbiome shape immunosurveillance and prognosis of proximal colon cancer.

Nature medicine

Roberti MP,Yonekura S,Duong CPM,Picard M,Ferrere G,Tidjani Alou M,Rauber C,Iebba V,Lehmann CHK,Amon L,Dudziak D,Derosa L,Routy B,Flament C,Richard C,Daillère R,Fluckiger A,Van Seuningen I,Chamaillard M,Vincent A,Kourula S,Opolon P,Ly P,Pizzato E,Becharef S,Paillet J,Klein C,Marliot F,Pietrantonio F,Benoist S,Scoazec JY,Dartigues P,Hollebecque A,Malka D,Pagès F,Galon J,Gomperts Boneca I,Lepage P,Ryffel B,Raoult D,Eggermont A,Vanden Berghe T,Ghiringhelli F,Vandenabeele P,Kroemer G,Zitvogel L

11-9942-82 was used in Flow cytometry/Cell sorting to demonstrate that immunogenic ileal apoptosis contributes to the prognosis of chemotherapy-treated CC.

Mon Jun 01 00:00:00 EDT 2020

Negative Co-stimulation Constrains T Cell Differentiation by Imposing Boundaries on Possible Cell States.

Immunity

Wei SC,Sharma R,Anang NAS,Levine JH,Zhao Y,Mancuso JJ,Setty M,Sharma P,Wang J,Pe'er D,Allison JP

11-9942 was used in Flow cytometry/Cell sorting to implicate negative co-stimulation as a key regulator and determinant of T cell differentiation and suggest that checkpoint blockade might work in part by altering the limits of T cell phenotypes.

Tue Apr 16 00:00:00 EDT 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9942-82) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Control of T_reg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells.

Nature communications

Chen S,Fang L,Guo W,Zhou Y,Yu G,Li W,Dong K,Liu J,Luo Y,Wang B,Li Z,Zhao C,Sun Z,Shen Y,Leng Q,Zhou D,Han Z,Huang H,Ren H,Xu G,Feng X

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9942-82) in Flow Cytometry

Thu Dec 13 00:00:00 EST 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9942-82) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2018

Dichotomous Expression of TNF Superfamily Ligands on Antigen-Presenting Cells Controls Post-priming Anti-viral CD4⁺ T Cell Immunity.

Immunity

Chang YH,Wang KC,Chu KL,Clouthier DL,Tran AT,Torres Perez MS,Zhou AC,Abdul-Sater AA,Watts TH

11-9942-82 was used in Flow Cytometry to investigate how different subtypes of T cell antigen-presenting cell contribute to determining viral set point and disease outcome.

Tue Nov 21 00:00:00 EST 2017

Effect of follicular helper T cells on the pathogenesis of asthma.

Experimental and therapeutic medicine

Ma X,Sun Z,Zhai P,Yu W,Wang T,Li F,Ding J

11-9942 was used in Flow cytometry/Cell sorting to investigate the effect of follicular helper T cells on bronchial asthma.

Tue Aug 01 00:00:00 EDT 2017

Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment.

Nature communications

Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9942-82) in Flow Cytometry

Mon May 15 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9942-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 11-9942-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

MyD88 signaling in T cells directs IgA-mediated control of the microbiota to promote health.

Cell host & microbe

Kubinak JL,Petersen C,Stephens WZ,Soto R,Bake E,O'Connell RM,Round JL

11-9942 was used in Flow cytometry/Cell sorting to show that signalling through the innate adaptor MyD88 in gut T cells coordinates germinal center responses, including T follicular helper cell and IgA+ B cell development.

Wed Feb 11 00:00:00 EST 2015

Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axis.

Nature immunology

Kim MS,Yan J,Wu W,Zhang G,Zhang Y,Cai D

11-9942 was used in Flow cytometry/Cell sorting to investigate the role of the brain in innate immunological signals, showing rapid linkage of innate signals and adaptive immunity by the brain-fat axis.

Fri May 01 00:00:00 EDT 2015

IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells.

Journal of immunology (Baltimore, Md. : 1950)

Ding Y,Li J,Wu Q,Yang P,Luo B,Xie S,Druey KM,Zajac AJ,Hsu HC,Mountz JD

11-9942 was used in Flow cytometry/Cell sorting to investigate the role of germinal centres in autoimmunity, showing that IL-17RA is essential for optimal localization of follicular Th cells in the germinal center light zone to promote autoantibody-producing B cells.

Thu Aug 15 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator

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Gene Aliases: AILIM; CCLP; CRP-1; H4; Icos; Ly115

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UniProt ID: (Mouse) Q9WVS0

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Entrez Gene ID: (Mouse) 54167

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Function(s)

protein binding

Process(es)

T cell tolerance induction single organismal cell-cell adhesion T cell costimulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD278 (ICOS) Monoclonal Antibody (7E.17G9), FITC, eBioscience™

CD278 (ICOS) Antibody (11-9942-82) in Flow

Product Details

11-9942-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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