Invitrogen

CD278 (ICOS) Monoclonal Antibody (7E.17G9), Biotin, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

CD278 (ICOS) Antibody (13-9942-85) in Flow

Staining of unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.03 µg of Rat IgG2b Isotype Control Biotin (Product # 13-4031-82) (open histogram) or 0.03 µg of Anti-Mouse CD278 (ICOS) Biotin followed by Streptavidin PE (Product # 12-4317-87). Viable cells were analyzed.

Product Image Gallery

Product Details

13-9942-85

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

7E.17G9

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467002

Product Specific Information

Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.

Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis.

Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and Con A-stimulated (3 day) mouse splenocytes and can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Control of T_reg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells.

Nature communications

Chen S,Fang L,Guo W,Zhou Y,Yu G,Li W,Dong K,Liu J,Luo Y,Wang B,Li Z,Zhao C,Sun Z,Shen Y,Leng Q,Zhou D,Han Z,Huang H,Ren H,Xu G,Feng X

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Thu Dec 13 00:00:00 EST 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2018

NCR1-deficiency diminishes the generation of protective murine cytomegalovirus antibodies by limiting follicular helper T-cell maturation.

European journal of immunology

Miletic A,Lenartic M,Popovic B,Brizic I,Trsan T,Miklic K,Mandelboim O,Krmpotic A,Jonjic S

13-9942 was used in Flow cytometry/Cell sorting to provide evidence for the role of NCR1 in antibody response to mouse cytomegalovirus infection.

Fri Sep 01 00:00:00 EDT 2017

Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment.

Nature communications

Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Mon May 15 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 13-9942-81) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Roquin recognizes a non-canonical hexaloop structure in the 3'-UTR of Ox40.

Nature communications

Janowski R,Heinz GA,Schlundt A,Wommelsdorf N,Brenner S,Gruber AR,Blank M,Buch T,Buhmann R,Zavolan M,Niessing D,Heissmeyer V,Sattler M

13-9942 was used in Flow cytometry/Cell sorting to reveal an unexpected recognition of hexaloop cis elements for the posttranscriptional regulation of target messenger RNAs by Roquin.

Thu Mar 24 00:00:00 EDT 2016

Neonatal follicular Th cell responses are impaired and modulated by IL-4.

Journal of immunology (Baltimore, Md. : 1950)

Debock I,Jaworski K,Chadlaoui H,Delbauve S,Passon N,Twyffels L,Leo O,Flamand V

13-9942 was used in Flow cytometry/Cell sorting to suggest that defective humoral immunity in early life is associated with limited and IL-4-modulated follicular Th cell responses.

Thu Aug 01 00:00:00 EDT 2013

The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding.

Nature immunology

Fazilleau N,McHeyzer-Williams LJ,Rosen H,McHeyzer-Williams MG

13-9942 was used in Flow cytometry/Cell sorting to study the antigen-specific mechanisms that program specialised effector follicular helper T cell function in vivo.

Wed Apr 01 00:00:00 EDT 2009

NKG2A inhibits invariant NKT cell activation in hepatic injury.

Journal of immunology (Baltimore, Md. : 1950)

Kawamura T,Takeda K,Kaneda H,Matsumoto H,Hayakawa Y,Raulet DH,Ikarashi Y,Kronenberg M,Yagita H,Kinoshita K,Abo T,Okumura K,Smyth MJ

13-9942 was used in Flow cytometry/Cell sorting to investigate the role of NK cell receptors in iNKT cell activation, showing that NKG2A-mediated signalling negatively regulated iNKT activation and hepatic injury.

Thu Jan 01 00:00:00 EST 2009

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator

View more View less

Gene Aliases: AILIM; CCLP; CRP-1; H4; Icos; Ly115

View more View less

UniProt ID: (Mouse) Q9WVS0

View more View less

Entrez Gene ID: (Mouse) 54167

View more View less

Function(s)

protein binding

Process(es)

T cell tolerance induction single organismal cell-cell adhesion T cell costimulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD278 (ICOS) Monoclonal Antibody (7E.17G9), Biotin, eBioscience™

CD278 (ICOS) Antibody (13-9942-85) in Flow

Product Details

13-9942-85

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!