Invitrogen

CD278 (ICOS) Monoclonal Antibody (7E.17G9), eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

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CD278 (ICOS) Antibody (14-9942-81) in Flow

Staining of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b Isotype Control Purified (Product # 14-4031-82) (open histogram) or 0.06 µg of Anti-Mouse CD278 (ICOS) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Total viable cells were used for analysis.

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Product Details

14-9942-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 16 publications 16 publications

Functional Assay (Functional)

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

7E.17G9

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468632

Product Specific Information

Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.

Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis and for inhibition of ligand binding in functional assays (please use Functional Grade purified, Product # 16-9942).

Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and Con A activated (3 day) mouse splenocytes and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9942-81) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Proenkephalin⁺ regulatory T cells expanded by ultraviolet B exposure maintain skin homeostasis with a healing function.

Proceedings of the National Academy of Sciences of the United States of America

Shime H,Odanaka M,Tsuiji M,Matoba T,Imai M,Yasumizu Y,Uraki R,Minohara K,Watanabe M,Bonito AJ,Fukuyama H,Ohkura N,Sakaguchi S,Morita A,Yamazaki S

14-9942-81 was used in Flow Cytometry to report a feature of skin Treg cells expanded by ultraviolet B (UVB) exposure.

Tue Aug 25 00:00:00 EDT 2020

Negative Co-stimulation Constrains T Cell Differentiation by Imposing Boundaries on Possible Cell States.

Immunity

Wei SC,Sharma R,Anang NAS,Levine JH,Zhao Y,Mancuso JJ,Setty M,Sharma P,Wang J,Pe'er D,Allison JP

14-9942 was used in Flow cytometry/Cell sorting to implicate negative co-stimulation as a key regulator and determinant of T cell differentiation and suggest that checkpoint blockade might work in part by altering the limits of T cell phenotypes.

Tue Apr 16 00:00:00 EDT 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9942-81) in Flow Cytometry

Tue Feb 20 00:00:00 EST 2018

Control of T_reg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells.

Nature communications

Chen S,Fang L,Guo W,Zhou Y,Yu G,Li W,Dong K,Liu J,Luo Y,Wang B,Li Z,Zhao C,Sun Z,Shen Y,Leng Q,Zhou D,Han Z,Huang H,Ren H,Xu G,Feng X

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9942-81) in Flow Cytometry

Thu Dec 13 00:00:00 EST 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9942-81) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2018

Dissection of SAP-dependent and SAP-independent SLAM family signaling in NKT cell development and humoral immunity.

The Journal of experimental medicine

Chen S,Cai C,Li Z,Liu G,Wang Y,Blonska M,Li D,Du J,Lin X,Yang M,Dong Z

14-9942-81 was used in Flow Cytometry to examine NKT cell development and humoral immunity in mice completely deficient in SFR.

Wed Feb 01 00:00:00 EST 2017

Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment.

Nature communications

Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9942-81) in Flow Cytometry

Mon May 15 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9942-81) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 14-9942-81) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Poxvirus-based active immunotherapy synergizes with CTLA-4 blockade to increase survival in a murine tumor model by improving the magnitude and quality of cytotoxic T cells.

Cancer immunology, immunotherapy : CII

Foy SP,Mandl SJ,dela Cruz T,Cote JJ,Gordon EJ,Trent E,Delcayre A,Breitmeyer J,Franzusoff A,Rountree RB

14-9942 was used in Flow cytometry/Cell sorting to help elucidate evidence of enhanced clinical benefit from combining CTLA-4 blockade with poxvirus-based active immunotherapy.

Sun May 01 00:00:00 EDT 2016

Interplay between regulatory T cells and PD-1 in modulating T cell exhaustion and viral control during chronic LCMV infection.

The Journal of experimental medicine

Penaloza-MacMaster P,Kamphorst AO,Wieland A,Araki K,Iyer SS,West EE,O'Mara L,Yang S,Konieczny BT,Sharpe AH,Freeman GJ,Rudensky AY,Ahmed R

14-9942 was used in Flow cytometry/Cell sorting to investigate a role for T reg cells in exhaustion of virus-specific CD8 T cells.

Mon Aug 25 00:00:00 EDT 2014

Effector CD4 T-cell transition to memory requires late cognate interactions that induce autocrine IL-2.

Nature communications

McKinstry KK,Strutt TM,Bautista B,Zhang W,Kuang Y,Cooper AM,Swain SL

14994281 was used in flow cytometry to explore the interaction between IL-2 and IL-7 in CD4 T-cell memory generation

Wed Nov 05 00:00:00 EST 2014

Cutaneous immunosurveillance and regulation of inflammation by group 2 innate lymphoid cells.

Nature immunology

Roediger B,Kyle R,Yip KH,Sumaria N,Guy TV,Kim BS,Mitchell AJ,Tay SS,Jain R,Forbes-Blom E,Chen X,Tong PL,Bolton HA,Artis D,Paul WE,Fazekas de St Groth B,Grimbaldeston MA,Le Gros G,Weninger W

14-9942 was used in Flow cytometry/Cell sorting to report the identification in normal mouse dermis of an abundant, phenotypically unique group 2 innate lymphoid cell subset that depended on interleukin 7 and constitutively produced IL-13.

Sat Jun 01 00:00:00 EDT 2013

B7-CD28 costimulatory signals control the survival and proliferation of murine and human γδ T cells via IL-2 production.

Journal of immunology (Baltimore, Md. : 1950)

Ribot JC,Debarros A,Mancio-Silva L,Pamplona A,Silva-Santos B

14-9942 was used in Blocking experiments to show that CD28 signals are required for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) γδ T cell subsets.

Wed Aug 01 00:00:00 EDT 2012

Dysregulated generation of follicular helper T cells in the spleen triggers fatal autoimmune hepatitis in mice.

Gastroenterology

Aoki N,Kido M,Iwamoto S,Nishiura H,Maruoka R,Tanaka J,Watanabe T,Tanaka Y,Okazaki T,Chiba T,Watanabe N

14-9942 was used in Functional assays to identify induction sites, responsible T-cell subsets, and key molecules for induction of autoimmune hepatitis.

Fri Apr 01 00:00:00 EDT 2011

The ICOS/ICOSL pathway is required for optimal antitumor responses mediated by anti-CTLA-4 therapy.

Cancer research

Fu T,He Q,Sharma P

14-9942 was used in Flow cytometry/Cell sorting to identify mechanisms that elicit clinical benefit in the setting of anti-CTLA-4 therapy.

Mon Aug 15 00:00:00 EDT 2011

Effector functions of donor-reactive CD8 memory T cells are dependent on ICOS induced during division in cardiac grafts.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Schenk AD,Gorbacheva V,Rabant M,Fairchild RL,Valujskikh A

14-9942 was used in Flow cytometry/Cell sorting to demonstrate that induction of ICOS expression within peripheral tissues is an important feature of CD8 memory T-cell activation.

Thu Jan 01 00:00:00 EST 2009

Differential effects of costimulatory pathway modulation on corneal allograft survival.

Investigative ophthalmology & visual science

Watson MP,George AJ,Larkin DF

14-9942 was used in Immunohistochemistry to examine the effect on corneal allograft survival of modulation of programmed death-1 and inducible costimulatory molecule.

Tue Aug 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator

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Gene Aliases: AILIM; CCLP; CRP-1; H4; Icos; Ly115

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UniProt ID: (Mouse) Q9WVS0

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Entrez Gene ID: (Mouse) 54167

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Function(s)

protein binding

Process(es)

T cell tolerance induction single organismal cell-cell adhesion T cell costimulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD278 (ICOS) Monoclonal Antibody (7E.17G9), eBioscience™

CD278 (ICOS) Antibody (14-9942-81) in Flow

Product Details

14-9942-81

Flow Cytometry (Flow)

Functional Assay (Functional)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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