Invitrogen

CD3 Monoclonal Antibody (UCHT1), PE, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

34 Published Figures

40 References

View all (504) CD3 antibodies

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CD3 Antibody (12-0038-42) in Flow

Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Product # 11-0199-42) and Mouse IgG1 kappa Isotype Control, PE (Product # 12-4714-82) (left) or CD3 Monoclonal Antibody, PE (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes. Details: Normal human whole blood was surface stained with CD3 (clone UCHT1). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis. {RE}

Product Details

12-0038-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 40 publications 40 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10667884

Product Specific Information

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: The UCHT1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Nanobody-derived bispecific CAR-T cell therapy enhances the anti-tumor efficacy of T cell lymphoma treatment.

Molecular therapy oncolytics

Xia B,Lin K,Wang X,Chen F,Zhou M,Li Y,Lin Y,Qiao Y,Li R,Zhang W,He X,Zou F,Li L,Lu L,Chen C,Li W,Zhang H,Liu B

12-0038-42 was used in Flow cytometry/Cell sorting to show that, because Nbs are smaller and less immunogenic, the synergistic effect of Nb-based bispecific CAR-T cells may improve their safety and efficacy in future clinical applications.

Thu Sep 21 00:00:00 EDT 2023

Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis.

iScience

Zhou J,Wang H,Shu T,Wang J,Yang W,Li J,Ding L,Liu M,Sun H,Wong J,Lai PB,Tsang SW,Ward SE,Chow KL,Sung JJ,Sze-Lok Cheng A

12-0038-42 was used in Flow cytometry/Cell sorting to demonstrated that cell cycle-related kinase (CCRK) is upregulated in myeloid cells in tumor-bearing mice and in patients with hepatocellular carcinoma.

Fri Oct 20 00:00:00 EDT 2023

WT1 Pulsed Human CD141+ Dendritic Cell Vaccine Has High Potential in Solid Tumor-Targeted Immunotherapy.

International journal of molecular sciences

Cho SY,Jeong SM,Jeon YJ,Yang SJ,Hwang JE,Yoo BM,Kim HS

12-0038-42 was used in Flow cytometry/Cell sorting to develop a DC vaccine named CellgramDC-WT1 (CDW).

Thu Jan 12 00:00:00 EST 2023

Aberrant gut-microbiota-immune-brain axis development in premature neonates with brain damage.

Cell host & microbe

Seki D,Mayer M,Hausmann B,Pjevac P,Giordano V,Goeral K,Unterasinger L,Klebermaß-Schrehof K,De Paepe K,Van de Wiele T,Spittler A,Kasprian G,Warth B,Berger A,Berry D,Wisgrill L

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Wed Oct 13 00:00:00 EDT 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Mon Jul 26 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

12-0038-42 was used in Flow Cytometry to show the feasibility and efficacy of human MUC16 ectodomain- specific BiTEDs and provide a basis for the combination with anti-VEGF therapy for ovarian cancer.

Thu Oct 21 00:00:00 EDT 2021

Mitochondrial Functions Are Compromised in CD4 T Cells From ART-Controlled PLHIV.

Frontiers in immunology

Zhao J,Schank M,Wang L,Li Z,Nguyen LN,Dang X,Cao D,Khanal S,Nguyen LNT,Thakuri BKC,Ogbu SC,Lu Z,Wu XY,Morrison ZD,Gazzar ME,Liu Y,Zhang J,Ning S,Moorman JP,Yao ZQ

12-0038 was used in Flow cytometry/Cell sorting to demonstrate a sequential cellular paradigm of T cell over-activation, proliferation, exhaustion, senescence, apoptosis, and depletion, which correlates with compromised mitochondrial functions.

Thu Sep 30 00:00:00 EDT 2021

Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma.

Cell research

Chen YP,Yin JH,Li WF,Li HJ,Chen DP,Zhang CJ,Lv JW,Wang YQ,Li XM,Li JY,Zhang PP,Li YQ,He QM,Yang XJ,Lei Y,Tang LL,Zhou GQ,Mao YP,Wei C,Xiong KX,Zhang HB,Zhu SD,Hou Y,Sun Y,Dean M,Amit I,Wu K,Kuang DM,Li GB,Liu N,Ma J

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

12-0038 was used in Flow cytometry/Cell sorting to investigate the effects of age on immunophenotype, proliferation, activation, and cytokine secretion capacities of PBMCs following co-culture with hPDLSCs.

Wed Jul 01 00:00:00 EDT 2020

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Wed Jul 01 00:00:00 EDT 2020

Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain.

The Journal of international medical research

He J,Xu Y,Yi M,Gu C,Zhu Y,Hu G

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Dendritic Cell Maturation Regulates TSPAN7 Function in HIV-1 Transfer to CD4⁺ T Lymphocytes.

Frontiers in cellular and infection microbiology

Perot BP,García-Paredes V,Luka M,Ménager MM

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Th2-like T Follicular Helper Cells Promote Functional Antibody Production during Plasmodium falciparum Infection.

Cell reports. Medicine

Chan JA,Loughland JR,de Labastida Rivera F,SheelaNair A,Andrew DW,Dooley NL,Wines BD,Amante FH,Webb L,Hogarth PM,McCarthy JS,Beeson JG,Engwerda CR,Boyle MJ

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Tue Dec 22 00:00:00 EST 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

12-0038-42 was used in Flow Cytometry to explore the possibility of targeting activated human blood T cells ex vivo, using the concept of photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA), a precursor of an endogenously synthesized photosensitizer protoporphyrin IX (PpIX) in combination with blue light.

Thu Feb 06 00:00:00 EST 2020

Loss of ARPC1B impairs cytotoxic T lymphocyte maintenance and cytolytic activity.

The Journal of clinical investigation

Randzavola LO,Strege K,Juzans M,Asano Y,Stinchcombe JC,Gawden-Bone CM,Seaman MN,Kuijpers TW,Griffiths GM

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Mon Dec 02 00:00:00 EST 2019

Skewed T cell responses to Epstein-Barr virus in long-term asymptomatic kidney transplant recipients.

PloS one

Nakid-Cordero C,Arzouk N,Gauthier N,Tarantino N,Larsen M,Choquet S,Burrel S,Autran B,Vieillard V,Guihot A

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Mon Mar 30 00:00:00 EDT 2020

Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.

Nature communications

Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Thu Nov 28 00:00:00 EST 2019

Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.

The Journal of clinical investigation

Maffucci P,Chavez J,Jurkiw TJ,O'Brien PJ,Abbott JK,Reynolds PR,Worth A,Notarangelo LD,Felgentreff K,Cortes P,Boisson B,Radigan L,Cobat A,Dinakar C,Ehlayel M,Ben-Omran T,Gelfand EW,Casanova JL,Cunningham-Rundles C

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Mon Dec 03 00:00:00 EST 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Guidelines for the use of flow cytometry and cell sorting in immunological studies.

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Akdis M,Andrä I,Annunziato F,Bacher P,Barnaba V,Battistini L,Bauer WM,Baumgart S,Becher B,Beisker W,Berek C,Blanco A,Borsellino G,Boulais PE,Brinkman RR,Büscher M,Busch DH,Bushnell TP,Cao X,Cavani A,Chattopadhyay PK,Cheng Q,Chow S,Clerici M,Cooke A,Cosma A,Cosmi L,Cumano A,Dang VD,Davies D,De Biasi S,Del Zotto G,Della Bella S,Dellabona P,Deniz G,Dessing M,Diefenbach A,Di Santo J,Dieli F,Dolf A,Donnenberg VS,Dörner T,Ehrhardt GRA,Endl E,Engel P,Engelhardt B,Esser C,Everts B,Dreher A,Falk CS,Fehniger TA,Filby A,Fillatreau S,Follo M,Förster I,Foster J,Foulds GA,Frenette PS,Galbraith D,Garbi N,García-Godoy MD,Geginat J,Ghoreschi K,Gibellini L,Goettlinger C,Goodyear CS,Gori A,Grogan J,Gross M,Grützkau A,Grummitt D,Hahn J,Hammer Q,Hauser AE,Haviland DL,Hedley D,Herrera G,Herrmann M,Hiepe F,Holland T,Hombrink P,Houston JP,Hoyer BF,Huang B,Hunter CA,Iannone A,Jäck HM,Jávega B,Jonjic S,Juelke K,Jung S,Kaiser T,Kalina T,Keller B,Khan S,Kienhöfer D,Kroneis T,Kunkel D,Kurts C,Kvistborg P,Lannigan J,Lantz O,Larbi A,LeibundGut-Landmann S,Leipold MD,Levings MK,Litwin V,Liu Y,Lohoff M,Lombardi G,Lopez L,Lovett-Racke A,Lubberts E,Ludewig B,Lugli E,Maecker HT,Martrus G,Matarese G,Maueröder C,McGrath M,McInnes I,Mei HE,Melchers F,Melzer S,Mielenz D,Mills K,Mirrer D,Mjösberg J,Moore J,Moran B,Moretta A,Moretta L,Mosmann TR,Müller S,Müller W,Münz C,Multhoff G,Munoz LE,Murphy KM,Nakayama T,Nasi M,Neudörfl C,Nolan J,Nourshargh S,O'Connor JE,Ouyang W,Oxenius A,Palankar R,Panse I,Peterson P,Peth C,Petriz J,Philips D,Pickl W,Piconese S,Pinti M,Pockley AG,Podolska MJ,Pucillo C,Quataert SA,Radstake TRDJ,Rajwa B,Rebhahn JA,Recktenwald D,Remmerswaal EBM,Rezvani K,Rico LG,Robinson JP,Romagnani C,Rubartelli A,Ruckert B,Ruland J,Sakaguchi S,Sala-de-Oyanguren F,Samstag Y,Sanderson S,Sawitzki B,Scheffold A,Schiemann M,Schildberg F,Schimisky E,Schmid SA,Schmitt S,Schober K,Schüler T,Schulz AR,Schumacher T,Scotta C,Shankey TV,Shemer A,Simon AK,Spidlen J,Stall AM,Stark R,Stehle C,Stein M,Steinmetz T,Stockinger H,Takahama Y,Tarnok A,Tian Z,Toldi G,Tornack J,Traggiai E,Trotter J,Ulrich H,van der Braber M,van Lier RAW,Veldhoen M,Vento-Asturias S,Vieira P,Voehringer D,Volk HD,von Volkmann K,Waisman A,Walker R,Ward MD,Warnatz K,Warth S,Watson JV,Watzl C,Wegener L,Wiedemann A,Wienands J,Willimsky G,Wing J,Wurst P,Yu L,Yue A,Zhang Q,Zhao Y,Ziegler S,Zimmermann J

12-0038 was used in Flow cytometry/Cell sorting to collect the currently accepted best methods for monitoring most of the variation of the major players of immune system.

Sun Oct 01 00:00:00 EDT 2017

Follicular Regulatory T Cells Are Highly Permissive to R5-Tropic HIV-1.

Journal of virology

Miller SM,Miles B,Guo K,Folkvord J,Meditz AL,McCarter MD,Levy DN,MaWhinney S,Santiago ML,Connick E

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

A Safety Checkpoint to Eliminate Cancer Risk of the Immune Evasive Cells Derived from Human Embryonic Stem Cells.

Stem cells (Dayton, Ohio)

He J,Rong Z,Fu X,Xu Y

Mon May 01 00:00:00 EDT 2017

Antigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities.

Oncoimmunology

Van Caeneghem Y,De Munter S,Tieppo P,Goetgeluk G,Weening K,Verstichel G,Bonte S,Taghon T,Leclercq G,Kerre T,Debets R,Vermijlen D,Abken H,Vandekerckhove B

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.

Arthritis research & therapy

Noack M,Ndongo-Thiam N,Miossec P

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

MAIT cells are activated during human viral infections.

Nature communications

van Wilgenburg B,Scherwitzl I,Hutchinson EC,Leng T,Kurioka A,Kulicke C,de Lara C,Cole S,Vasanawathana S,Limpitikul W,Malasit P,Young D,Denney L,Moore MD,Fabris P,Giordani MT,Oo YH,Laidlaw SM,Dustin LB,Ho LP,Thompson FM,Ramamurthy N,Mongkolsapaya J,Willberg CB,Screaton GR,Klenerman P

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

Antibodies to P-selectin glycoprotein ligand-1 block dendritic cell-mediated enterovirus 71 transmission and prevent virus-induced cells death.

Virulence

Ren XX,Li C,Xiong SD,Huang Z,Wang JH,Wang HB

12-0038 was used in Flow cytometry/Cell sorting to demonstrate that PSGL-1 present on the cell surfaces of monocyte-derived dendritic cells mediates enterovirus 71 binding and facilitates further infection.

Thu Oct 06 00:00:00 EDT 2016

Regulatory Dendritic Cells Restrain NK Cell IFN-γ Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.

Journal of immunology (Baltimore, Md. : 1950)

Spallanzani RG,Torres NI,Avila DE,Ziblat A,Iraolagoitia XL,Rossi LE,Domaica CI,Fuertes MB,Rabinovich GA,Zwirner NW

12-0038 was used in Flow cytometry/Cell sorting to investigate the functional outcome of the interaction between regDC and NK cells and the associated underlying mechanisms.

Tue Sep 01 00:00:00 EDT 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

Oncogenic Transformation Can Orchestrate Immune Evasion and Inflammation in Human Mesenchymal Stem Cells Independently of Extrinsic Immune-Selective Pressure.

Cancer research

Miranda A,Funes JM,Sánchez N,Limia CM,Mesa M,Quezada SA,Pérez R,de León J

12-0038-42 was used in flow cytometry experiments to investigate mesenchymal stem cells in oncogenic immune evasion.

Sat Aug 01 00:00:00 EDT 2015

Depressive symptoms post hip fracture in older adults are associated with phenotypic and functional alterations in T cells.

Immunity & ageing : I & A

Duggal NA,Upton J,Phillips AC,Hampson P,Lord JM

12-0038 was used in Flow cytometry/Cell sorting to investigate the synergistic effects of hip fracture physical trauma and psychological depressive symptoms on the aged immune system.

Wed Jan 28 00:00:00 EST 2015

Human mesenchymal stromal cells modulate T-cell responses through TNF-α-mediated activation of NF-κB.

European journal of immunology

Dorronsoro A,Ferrin I,Salcedo JM,Jakobsson E,Fernández-Rueda J,Lang V,Sepulveda P,Fechter K,Pennington D,Trigueros C

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2014

A comparison of DNA methylation specific droplet digital PCR (ddPCR) and real time qPCR with flow cytometry in characterizing human T cells in peripheral blood.

Epigenetics

Wiencke JK,Bracci PM,Hsuang G,Zheng S,Hansen H,Wrensch MR,Rice T,Eliot M,Kelsey KT

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

Trypan blue exclusion assay by flow cytometry.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Avelar-Freitas BA,Almeida VG,Pinto MC,Mourão FA,Massensini AR,Martins-Filho OA,Rocha-Vieira E,Brito-Melo GE

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Tue Apr 01 00:00:00 EDT 2014

Induction of type I and type III interferons by Borrelia burgdorferi correlates with pathogenesis and requires linear plasmid 36.

PloS one

Krupna-Gaylord MA,Liveris D,Love AC,Wormser GP,Schwartz I,Petzke MM

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Thu Mar 12 00:00:00 EDT 2015

Novel lentiviral vectors with mutated reverse transcriptase for mRNA delivery of TALE nucleases.

Scientific reports

Mock U,Riecken K,Berdien B,Qasim W,Chan E,Cathomen T,Fehse B

12-0038 was used in Flow cytometry/Cell sorting to develop a novel lentiviral vector-based TALEN transfer system by introducing a non-functional reverse transcriptase.

Thu Sep 18 00:00:00 EDT 2014

Recombinant influenza virus carrying the respiratory syncytial virus (RSV) F85-93 CTL epitope reduces RSV replication in mice.

Journal of virology

De Baets S,Schepens B,Sedeyn K,Schotsaert M,Roose K,Bogaert P,Fiers W,Saelens X

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

MHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy.

PloS one

Dössinger G,Bunse M,Bet J,Albrecht J,Paszkiewicz PJ,Weißbrich B,Schiedewitz I,Henkel L,Schiemann M,Neuenhahn M,Uckert W,Busch DH

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Mon Nov 11 00:00:00 EST 2013

Epigenetic biomarkers of T-cells in human glioma.

Epigenetics

Wiencke JK,Accomando WP,Zheng S,Patoka J,Dou X,Phillips JJ,Hsuang G,Christensen BC,Houseman EA,Koestler DC,Bracci P,Wiemels JL,Wrensch M,Nelson HH,Kelsey KT

Published figure using CD3 monoclonal antibody (Product # 12-0038-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2012

Penicillium marneffei-stimulated dendritic cells enhance HIV-1 trans-infection and promote viral infection by activating primary CD4+ T cells.

PloS one

Qin Y,Li Y,Liu W,Tian R,Guo Q,Li S,Li H,Zhang D,Zheng Y,Wu L,Lan K,Wang J

12-0038 was used in Flow cytometry/Cell sorting to demonstrate that DC interaction with HIV-1 has been exploited by P. marneffei for viral amplification.

Mon Mar 26 00:00:00 EDT 2012

Replication of Epstein-Barr virus primary infection in human tonsil tissue explants.

PloS one

Gotoh K,Ito Y,Maruo S,Takada K,Mizuno T,Teranishi M,Nakata S,Nakashima T,Iwata S,Goshima F,Nakamura S,Kimura H

12-0038 was used in Flow cytometry/Cell sorting to assess the ability of B95-8 virus and recombinant Epstein-Barr virus-eGFP to replicate in human lymphoid tissue, to establish a model for the study of viral infection.

Mon Feb 13 00:00:00 EST 2012

An antibody against the surfactant protein A (SP-A)-binding domain of the SP-A receptor inhibits T cell-mediated immune responses to Mycobacterium tuberculosis.

Journal of leukocyte biology

Samten B,Townsend JC,Sever-Chroneos Z,Pasquinelli V,Barnes PF,Chroneos ZC

12-0038 was used in Flow cytometry/Cell sorting to study the effect of antibodies on T cell immune responses against M.tuberculosis.

Tue Jul 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

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CD3 Monoclonal Antibody (UCHT1), PE, eBioscience™

CD3 Antibody (12-0038-42) in Flow

Product Details

12-0038-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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Bioinformatics

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