Invitrogen

CD3 Monoclonal Antibody (UCHT1), PE-Cyanine7, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

44 References

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CD3 Antibody (25-0038-42) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD19 FITC and CD3 PE-Cyanine5. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes. Details: Normal human whole blood was surface stained with CD3 (clone UCHT1). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis. {RE}

Product Details

25-0038-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 41 publications 41 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1582253

Product Specific Information

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: This UCHT1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Tumor cell-intrinsic and tumor microenvironmental conditions co-determine signaling by the glycoimmune checkpoint receptor Siglec-7.

Cellular and molecular life sciences : CMLS

van Houtum EJH,Kers-Rebel ED,Looman MW,Hooijberg E,Büll C,Granado D,Cornelissen LAM,Adema GJ

25-0038-42 was used in Flow cytometry/Cell sorting to define a set of tumor-associated conditions that influence Siglec-7 signaling.

Tue May 30 00:00:00 EDT 2023

Oncolytic virus driven T-cell-based combination immunotherapy platform for colorectal cancer.

Frontiers in immunology

Crupi MJF,Taha Z,Janssen TJA,Petryk J,Boulton S,Alluqmani N,Jirovec A,Kassas O,Khan ST,Vallati S,Lee E,Huang BZ,Huh M,Pikor L,He X,Marius R,Austin B,Duong J,Pelin A,Neault S,Azad T,Breitbach CJ,Stojdl DF,Burgess MF,McComb S,Auer R,Diallo JS,Ilkow CS,Bell JC

25-0038-42 was used in Flow Cytometry to establish combination regimens with immune checkpoint inhibitors for aggressive colorectal peritoneal metastases.

Tue Nov 22 00:00:00 EST 2022

Isolation and Quantification of Mouse γδT-cells in vitro and in vivo.

Bio-protocol

Rana I,Badarinath K,Zirmire RK,Jamora C

25-0038-42 was used in Flow cytometry/Cell sorting to describe a protocol to isolate and culture DETCs and analyse them in vivo within the skin.

Sun Sep 05 00:00:00 EDT 2021

Lysine-specific demethylase 1A restricts ex vivo propagation of human HSCs and is a target of UM171.

Blood

Subramaniam A,Žemaitis K,Talkhoncheh MS,Yudovich D,Bäckström A,Debnath S,Chen J,Jain MV,Galeev R,Gaetani M,Zubarev RA,Larsson J

25-0038 was used in Flow cytometry/Cell sorting to conclude that LSD1 and CoREST are principal targets of UM171-mediated HSC expansion.

Thu Nov 05 00:00:00 EST 2020

Efficient and nontoxic biomolecule delivery to primary human hematopoietic stem cells using nanostraws.

Proceedings of the National Academy of Sciences of the United States of America

Schmiderer L,Subramaniam A,Žemaitis K,Bäckström A,Yudovich D,Soboleva S,Galeev R,Prinz CN,Larsson J,Hjort M

25-0038 was used in Flow cytometry/Cell sorting to show that nanostraw-mediated transfection is a gentle alternative to established gene delivery methods, and uniquely suited for nonperturbative treatment of sensitive primary stem cells.

Tue Sep 01 00:00:00 EDT 2020

Expansion of Group 2 Innate Lymphoid Cells in Patients with End-Stage Renal Disease and Their Clinical Significance.

Journal of immunology (Baltimore, Md. : 1950)

Liu GY,Deng XH,Li X,Cao YJ,Xing YF,Zhou P,Lei AH,Yang Q,Deng K,Zhang H,Zhou J

25-0038-42 was used in Flow Cytometry to indicate that ILC2s may play a protective role in ESRD.

Wed Jul 01 00:00:00 EDT 2020

Human pDCs Are Superior to cDC2s in Attracting Cytolytic Lymphocytes in Melanoma Patients Receiving DC Vaccination.

Cell reports

van Beek JJP,Flórez-Grau G,Gorris MAJ,Mathan TSM,Schreibelt G,Bol KF,Textor J,de Vries IJM

25-0038 was used in Flow cytometry/Cell sorting to show that pDCs and cDC2s display a striking difference in chemokine secretion, which correlates with the recruitment of distinct types of immune effector cells.

Tue Jan 28 00:00:00 EST 2020

Scalable high-throughput acoustophoresis in arrayed plastic microchannels.

Biomicrofluidics

Dubay R,Lissandrello C,Swierk P,Moore N,Doty D,Fiering J

25-0038 was used in Flow cytometry/Cell sorting to suggest that acoustophoresis could in the future aid in cell therapy bioprocessing and that further scale-up is possible.

Wed May 01 00:00:00 EDT 2019

Immunosuppression by monocytic myeloid-derived suppressor cells in patients with pancreatic ductal carcinoma is orchestrated by STAT3.

Journal for immunotherapy of cancer

Trovato R,Fiore A,Sartori S,Canè S,Giugno R,Cascione L,Paiella S,Salvia R,De Sanctis F,Poffe O,Anselmi C,Hofer F,Sartoris S,Piro G,Carbone C,Corbo V,Lawlor R,Solito S,Pinton L,Mandruzzato S,Bassi C,Scarpa A,Bronte V,Ugel S

25-0038 was used in Flow cytometry/Cell sorting to report that PDAC patients show increased levels of both circulating and tumor-infiltrating MDSC-like cells.

Wed Sep 18 00:00:00 EDT 2019

Expression of PD-1 by T Cells in Malignant Glioma Patients Reflects Exhaustion and Activation.

Clinical cancer research : an official journal of the American Association for Cancer Research

Davidson TB,Lee A,Hsu M,Sedighim S,Orpilla J,Treger J,Mastall M,Roesch S,Rapp C,Galvez M,Mochizuki A,Antonios J,Garcia A,Kotecha N,Bayless N,Nathanson D,Wang A,Everson R,Yong WH,Cloughesy TF,Liau LM,Herold-Mende C,Prins RM

25-0038-42 was used in Flow cytometry/Cell sorting to suggests that PD-1 expression in patients with glioma reflects chronically activated effector T cells that display hallmarks of memory and exhaustion depending on its anatomic location.

Fri Mar 15 00:00:00 EDT 2019

The Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma.

Journal of immunology research

Zhao X,Hua M,Yan S,Yu J,Han F,Zhong C,Wang R,Zhang C,Hou M,Ma D

25-0038 was used in Flow cytometry/Cell sorting to investigate the role of the NLRP3 inflammasome in multiple myeloma.

Fri Nov 09 00:00:00 EST 2018

The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.

Acta Cardiologica Sinica

Ding JW,Zhou T,Zheng XX,Wang XA,Tong XH,Luo CY,Zhang ZQ,Yu B

25-0038 was used in Flow cytometry/Cell sorting to explore the mechanism of regulatory T cell/Th17 imbalance and the role of high mobility group box-1 protein on the balance in atherosclerosis.

Sat Sep 01 00:00:00 EDT 2018

Neisseria gonorrhoeae co-infection exacerbates vaginal HIV shedding without affecting systemic viral loads in human CD34+ engrafted mice.

PloS one

Xu SX,Leontyev D,Kaul R,Gray-Owen SD

Mon Feb 26 00:00:00 EST 2018

EDAG promotes the expansion and survival of human CD34+ cells.

PloS one

Zhao K,Zheng WW,Dong XM,Yin RH,Gao R,Li X,Liu JF,Zhan YQ,Yu M,Chen H,Ge CH,Ning HM,Yang XM,Li CY

25-0038 was used in Flow cytometry/Cell sorting to provide novel insight into EDAG in regulation of expansion and survival of human hematopoietic stem/progenitor cells.

Tue Feb 06 00:00:00 EST 2018

Broad CD8⁺ T cell cross-recognition of distinct influenza A strains in humans.

Nature communications

Grant EJ,Josephs TM,Loh L,Clemens EB,Sant S,Bharadwaj M,Chen W,Rossjohn J,Gras S,Kedzierska K

25-0038 was used in Flow cytometry/Cell sorting to investigate TCRαΒ cross-strain recognition across IAV variants within two immunodominant human Influenza A virus(IAV)-specific CD8+ T-cell epitopes, HLA-B*37:01-restricted NP338-346 (B37-NP338) and HLA-A*01:01-restricted NP44-52 (A1-NP44).

Fri Dec 21 00:00:00 EST 2018

Immunomodulatory Drugs in the Context of Autologous Hematopoietic Stem Cell Transplantation Associate With Reduced Pro-tumor T Cell Subsets in Multiple Myeloma.

Frontiers in immunology

Di Lullo G,Marcatti M,Heltai S,Tresoldi C,Paganoni AM,Bordignon C,Ciceri F,Protti MP

25-0038 was used in Flow cytometry/Cell sorting to study the role of immunomodulatory drugs in the context of autologous hematopoietic stem cell transplantation on the T cell subset distribution in the bone marrow of newly diagnosed multiple myeloma patients.

Mon Oct 14 00:00:00 EDT 2019

ILC2-modulated T cell-to-MDSC balance is associated with bladder cancer recurrence.

The Journal of clinical investigation

Chevalier MF,Trabanelli S,Racle J,Salomé B,Cesson V,Gharbi D,Bohner P,Domingos-Pereira S,Dartiguenave F,Fritschi AS,Speiser DE,Rentsch CA,Gfeller D,Jichlinski P,Nardelli-Haefliger D,Jandus C,Derré L

25-0038 was used in Flow cytometry/Cell sorting to underline the ILC2/IL-13 axis as a targetable pathway to curtail the M-MDSC compartment and improve bladder cancer treatment.

Tue Aug 01 00:00:00 EDT 2017

ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.

Frontiers in immunology

Lecciso M,Ocadlikova D,Sangaletti S,Trabanelli S,De Marchi E,Orioli E,Pegoraro A,Portararo P,Jandus C,Bontadini A,Redavid A,Salvestrini V,Romero P,Colombo MP,Di Virgilio F,Cavo M,Adinolfi E,Curti A

25-0038 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment.

Thu Mar 31 00:00:00 EDT 2022

Intact CD100-CD72 Interaction Necessary for TCR-Induced T Cell Proliferation.

Frontiers in immunology

Jiang X,Björkström NK,Melum E

25-0038 was used in Flow cytometry/Cell sorting to reveal that T cell proliferation is regulated by CD100 via interaction with CD72 and further establish an in vitro system to evaluate the inhibitory effect of anti-CD100 antibodies on T-cells.

Wed Nov 20 00:00:00 EST 2019

Early changes within the lymphocyte population are associated with the development of multiple organ dysfunction syndrome in trauma patients.

Critical care (London, England)

Manson J,Cole E,De'Ath HD,Vulliamy P,Meier U,Pennington D,Brohi K

25-0038 was used in Flow cytometry/Cell sorting to study how early changes within the lymphocyte population are associated with the development of multiple organ dysfunction syndrome in trauma patients.

Tue Jun 07 00:00:00 EDT 2016

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

PLoS pathogens

Miles B,Miller SM,Folkvord JM,Levy DN,Rakasz EG,Skinner PJ,Connick E

25-0038 was used in Flow cytometry/Cell sorting to demonstrate that a unique regulatory CD8 population exists in follicles that impairs GC function in HIV infection.

Sat Oct 01 00:00:00 EDT 2016

Antithymocyte Globulin at Clinically Relevant Concentrations Kills Leukemic Blasts.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Dabas R,Lee R,Servito MT,Dharmani-Khan P,Modi M,van Slyke T,Luider J,Durand C,Larratt L,Brandwein J,Morris D,Daly A,Khan FM,Storek J

Sun May 01 00:00:00 EDT 2016

Development of depressive symptoms post hip fracture is associated with altered immunosuppressive phenotype in regulatory T and B lymphocytes.

Biogerontology

Duggal NA,Upton J,Phillips AC,Lord JM

25-0038 was used in Flow cytometry/Cell sorting to investigate whether hip fracture and depressive symptoms had additive effects upon the aged immune system.

Mon Feb 01 00:00:00 EST 2016

Frequencies of Circulating MAIT Cells Are Diminished in Chronic HCV, HIV and HCV/HIV Co-Infection and Do Not Recover during Therapy.

PloS one

Spaan M,Hullegie SJ,Beudeker BJ,Kreefft K,van Oord GW,Groothuismink ZM,van Tilborg M,Rijnders B,de Knegt RJ,Claassen MA,Boonstra A

25-0038 was used in Flow cytometry/Cell sorting to show that the frequencies of MAIT cells are reduced in blood of patients with CHCV, HIV and in AHCV/HIV co-infection compared to healthy individuals.

Wed Jul 19 00:00:00 EDT 2017

Depressive symptoms post hip fracture in older adults are associated with phenotypic and functional alterations in T cells.

Immunity & ageing : I & A

Duggal NA,Upton J,Phillips AC,Hampson P,Lord JM

25-0038 was used in Flow cytometry/Cell sorting to investigate the synergistic effects of hip fracture physical trauma and psychological depressive symptoms on the aged immune system.

Wed Jan 28 00:00:00 EST 2015

Journal of immunology (Baltimore, Md. : 1950)

Britanova OV,Putintseva EV,Shugay M,Merzlyak EM,Turchaninova MA,Staroverov DB,Bolotin DA,Lukyanov S,Bogdanova EA,Mamedov IZ,Lebedev YB,Chudakov DM

25-0038 was used in Flow cytometry/Cell sorting to demonstrate that TCR β diversity decreases roughly linearly with age.

Sat Mar 15 00:00:00 EDT 2014

A truncation variant of the cation channel P2RX5 is upregulated during T cell activation.

PloS one

Abramowski P,Ogrodowczyk C,Martin R,Pongs O

25-0038 was used in Flow cytometry/Cell sorting to show that P2RX5 gene expression of human T lymphocytes is upregulated during activation.

Mon May 18 00:00:00 EDT 2015

The orally available, synthetic ether lipid edelfosine inhibits T cell proliferation and induces a type I interferon response.

PloS one

Abramowski P,Otto B,Martin R

25-0038 was used in Flow cytometry/Cell sorting to study how the inhibition of homeostatic proliferation, MHC class II expression and presentation, and the induction of type I interferon-associated genes could be used to develop edelfosine for clinical use in MS.

Thu Jan 22 00:00:00 EST 2015

The nucleotide sugar UDP-glucose mobilizes long-term repopulating primitive hematopoietic cells.

The Journal of clinical investigation

Kook S,Cho J,Lee SB,Lee BC

25-0038 was used in Flow cytometry/Cell sorting to demonstrate a novel mechanism governing the migration of haematopoietic stem progenitor cells under stress.

Thu Aug 01 00:00:00 EDT 2013

HLA-DR15-derived self-peptides are involved in increased autologous T cell proliferation in multiple sclerosis.

Brain : a journal of neurology

Mohme M,Hotz C,Stevanovic S,Binder T,Lee JH,Okoniewski M,Eiermann T,Sospedra M,Rammensee HG,Martin R

25-0038 was used in Flow cytometry/Cell sorting to investigate perturbed T cell repertoire dynamics and the functional involvement of the major genetic risk factor, the HLA-DR15 haplotype, in multiple sclerosis.

Sat Jun 01 00:00:00 EDT 2013

Mother and child T cell receptor repertoires: deep profiling study.

Frontiers in immunology

Putintseva EV,Britanova OV,Staroverov DB,Merzlyak EM,Turchaninova MA,Shugay M,Bolotin DA,Pogorelyy MV,Mamedov IZ,Bobrynina V,Maschan M,Lebedev YB,Chudakov DM

25-0038 was used in Flow cytometry/Cell sorting to perform deep quantitative profiling of T cell receptor repertoires from the peripheral blood samples of three mothers and their six children.

Wed Jan 08 00:00:00 EST 2014

A Novel Method Linking Antigen Presentation by Human Monocyte-Derived Macrophages to CD8(+) T Cell Polyfunctionality.

Frontiers in immunology

Short KR,Grant EJ,Vissers M,Reading PC,Diavatopoulos DA,Kedzierska K

25-0038 was used in Flow cytometry/Cell sorting to study how virally infected macrophages impact T cell function.

Fri Dec 06 00:00:00 EST 2013

Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.

Journal of immunology (Baltimore, Md. : 1950)

Palin AC,Ramachandran V,Acharya S,Lewis DB

25-0038 was used in Flow cytometry/Cell sorting to investigate the developmental differences in immune function between human neonates and adults, showing that neonatal naive CD4+ T cells have enhanced activation-dependent signalling.

Fri Mar 15 00:00:00 EDT 2013

Incomplete normalization of regulatory t-cell frequency in the gut mucosa of Colombian HIV-infected patients receiving long-term antiretroviral treatment.

PloS one

Rueda CM,Velilla PA,Chougnet CA,Rugeles MT

25-0038 was used in Functional assays to assess the frequency and phenotype of regulatory T-cells within CMV and HIV patients on different treatment regimes.

Tue Sep 23 00:00:00 EDT 2014

Preserved function of regulatory T cells in chronic HIV-1 infection despite decreased numbers in blood and tissue.

The Journal of infectious diseases

Angin M,Kwon DS,Streeck H,Wen F,King M,Rezai A,Law K,Hongo TC,Pyo A,Piechocka-Trocha A,Toth I,Pereyra F,Ghebremichael M,Rodig SJ,Milner DA,Richter JM,Altfeld M,Kaufmann DE,Walker BD,Addo MM

25-0038 was used in Flow cytometry/Cell sorting to study the frequency and function of Tregs in HIV-1-infected individuals.

Tue May 15 00:00:00 EDT 2012

Histone deacetylase inhibitors impair NK cell viability and effector functions through inhibition of activation and receptor expression.

Journal of leukocyte biology

Rossi LE,Avila DE,Spallanzani RG,Ziblat A,Fuertes MB,Lapyckyj L,Croci DO,Rabinovich GA,Domaica CI,Zwirner NW

25-0038 was used in Flow cytometry/Cell sorting to investigate the effect of histone deacetylase inhibitors on immune cells, showing that they can impair NK cell viability and effector functions.

Wed Feb 01 00:00:00 EST 2012

Ulcerative colitis impairs the acylethanolamide-based anti-inflammatory system reversal by 5-aminosalicylic acid and glucocorticoids.

PloS one

Suárez J,Romero-Zerbo Y,Márquez L,Rivera P,Iglesias M,Bermúdez-Silva FJ,Andreu M,Rodríguez de Fonseca F

25-0038 was used in Immunofluorescence to study how the NAE-PPARα signaling system is impaired during active UC and 5-ASA/glucocorticoids treatment restored its normal expression.

Thu Dec 13 00:00:00 EST 2012

HIV-induced T-cell activation/exhaustion in rectal mucosa is controlled only partially by antiretroviral treatment.

PloS one

Rueda CM,Velilla PA,Chougnet CA,Montoya CJ,Rugeles MT

25-0038 was used in Flow cytometry/Cell sorting to investigate the effect of chronic HIV infection on the immune cell phenotype.

Mon Jun 04 00:00:00 EDT 2012

Chronically HIV-1 Infected Patients Exhibit Low Frequencies of CD25+ Regulatory T Cells.

The open virology journal

Rios CM,Velilla PA,Rugeles MT

25-0038 was used in Flow cytometry/Cell sorting to investigate the role of Tregs during HIV infection, showing that chronically HIV-1 infected patients exhibit low frequencies of CD25+ Tregs.

Thu Aug 23 00:00:00 EDT 2012

Central role of JC virus-specific CD4+ lymphocytes in progressive multi-focal leucoencephalopathy-immune reconstitution inflammatory syndrome.

Brain : a journal of neurology

Aly L,Yousef S,Schippling S,Jelcic I,Breiden P,Matschke J,Schulz R,Bofill-Mas S,Jones L,Demina V,Linnebank M,Ogg G,Girones R,Weber T,Sospedra M,Martin R

25-0038 was used in Flow cytometry/Cell sorting to provide novel insight into the pathogenesis of progressive multi-focal leucoencephalopathy-immune reconstitution inflammatory syndrome.

Thu Sep 01 00:00:00 EDT 2011

Differential expression of CD300a/c on human TH1 and TH17 cells.

BMC immunology

Simhadri VR,Mariano JL,Zhou Q,DeBell KE,Borrego F

25-0038 was used in Flow cytometry/Cell sorting to conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells, and that their ligation is capable of modulating TCR mediated signals.

Wed Nov 02 00:00:00 EDT 2011

Accumulation of natural killer T cells in progressive nonalcoholic fatty liver disease.

Hepatology (Baltimore, Md.)

Syn WK,Oo YH,Pereira TA,Karaca GF,Jung Y,Omenetti A,Witek RP,Choi SS,Guy CD,Fearing CM,Teaberry V,Pereira FE,Adams DH,Diehl AM

25-0038 was used in Immunocytochemistry to show that hedgehog pathway activation leads to hepatic enrichment with NKT cells that contribute to fibrosis progression in nonalcoholic steatohepatitis.

Tue Jun 01 00:00:00 EDT 2010

Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.

Journal of immunology (Baltimore, Md. : 1950)

Oo YH,Weston CJ,Lalor PF,Curbishley SM,Withers DR,Reynolds GM,Shetty S,Harki J,Shaw JC,Eksteen B,Hubscher SG,Walker LS,Adams DH

25-0038 was used in Flow cytometry/Cell sorting to investigate the molecular control of Treg recruitment into inflamed human tissue.

Mon Mar 15 00:00:00 EDT 2010

Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.

PloS one

Narayanan S,Silva R,Peruzzi G,Alvarez Y,Simhadri VR,Debell K,Coligan JE,Borrego F

25-0038 was used in Functional assays to investigate a subset within the CD4 T cell population defined by expression of the immunomodulatory receptor CD300a.

Thu May 13 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

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CD3 Monoclonal Antibody (UCHT1), PE-Cyanine7, eBioscience™

CD3 Antibody (25-0038-42) in Flow

Product Details

25-0038-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

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Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

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RRID

Product Specific Information

Target Information

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