Invitrogen

CD3 Monoclonal Antibody (UCHT1), Alexa Fluor™ 700, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

24 References

View all (504) CD3 antibodies

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Datasheet

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Antibody Testing Data (2)
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CD3 Antibody (56-0038-42) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD19 FITC and CD3 Alexa Fluor 700. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes. Details: Normal human whole blood was surface stained with CD3 (clone UCHT1). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis. {RE}

Product Details

56-0038-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Conjugate

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

Excitation/Emission Max

696/719 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10597906

Product Specific Information

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex.Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex.CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells.Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported:This UCHT1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: Has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 mm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The XIST lncRNA is a sex-specific reservoir of TLR7 ligands in SLE.

JCI insight

Crawford JD,Wang H,Trejo-Zambrano D,Cimbro R,Talbot CC,Thomas MA,Curran AM,Girgis AA,Schroeder JT,Fava A,Goldman DW,Petri M,Rosen A,Antiochos B,Darrah E

56-0038-42 was used in Flow cytometry/Cell sorting to suggest a novel role for XIST RNA as a female-specific danger signal underlying the sex bias in SLE.

Thu Sep 21 00:00:00 EDT 2023

Ex vivo assays show human gamma-delta T cells specific for common allergens are Th1-polarized in allergic donors.

Cell reports methods

Yu ED,Wang E,Garrigan E,Sutherland A,Khalil N,Kearns K,Pham J,Schulten V,Peters B,Frazier A,Sette A,da Silva Antunes R

56-0038-42 was used in flow cytometry to support the existence of allergen-reactive γδ T cells and their potential use in rebalancing dysregulated Th2 responses in allergic diseases.

Mon Dec 19 00:00:00 EST 2022

Effects of dexmedetomidine on immune response in patients undergoing radical and reconstructive surgery for oral cancer.

Oncology letters

Huang L,Qin C,Wang L,Zhang T,Li J

56-0038 was used in Flow cytometry/Cell sorting to suggest that dexmedetomidine can attenuate immunosuppression in patients undergoing radical and reconstructive surgery for oral cancer.

Mon Feb 01 00:00:00 EST 2021

Comprehensive analysis of T cell immunodominance and immunoprevalence of SARS-CoV-2 epitopes in COVID-19 cases.

Cell reports. Medicine

Tarke A,Sidney J,Kidd CK,Dan JM,Ramirez SI,Yu ED,Mateus J,da Silva Antunes R,Moore E,Rubiro P,Methot N,Phillips E,Mallal S,Frazier A,Rawlings SA,Greenbaum JA,Peters B,Smith DM,Crotty S,Weiskopf D,Grifoni A,Sette A

56-0038-42 was used in Flow Cytometry to identify several hundred HLA-restricted SARS-CoV-2-derived epitopes.

Tue Feb 16 00:00:00 EST 2021

A Versatile Protocol to Quantify BCR-mediated Phosphorylation in Human and Murine B Cell Subpopulations.

Bio-protocol

Rip J,Hendriks RW,Corneth OBJ

56-0038-42 was used in Flow Cytometry to present a step-by-step phosphoflow protocol allowing the evaluation of the phosphorylation status of signaling molecules in conjunction with extensive staining to identify various human and murine B cell subpopulations, as was previously published in the original paper by Rip et al.

Fri Feb 05 00:00:00 EST 2021

Impact of SARS-CoV-2 variants on the total CD4⁺ and CD8⁺ T cell reactivity in infected or vaccinated individuals.

Cell reports. Medicine

Tarke A,Sidney J,Methot N,Yu ED,Zhang Y,Dan JM,Goodwin B,Rubiro P,Sutherland A,Wang E,Frazier A,Ramirez SI,Rawlings SA,Smith DM,da Silva Antunes R,Peters B,Scheuermann RH,Weiskopf D,Crotty S,Grifoni A,Sette A

56-0038-42 was used in Flow Cytometry to show SARS-CoV-2 variants do not significantly disrupt SARS-CoV-2 T cell reactivity.

Tue Jul 20 00:00:00 EDT 2021

Pre-existing T Cell Memory against Zika Virus.

Journal of virology

Schouest B,Grifoni A,Pham J,Mateus J,Sydney J,Brien JD,De Silva AD,Balmaseda A,Harris E,Sette A,Weiskopf D

56-0038-42 was used in Flow cytometry/Cell sorting to show that in DENV-exposed donors, preexisting immunity to DENV could shape ZIKV-specific responses, and DENV-ZIKV cross-reactive T cells can be expanded by stimulation with ZIKV peptides.

Mon May 24 00:00:00 EDT 2021

Single-Cell Transcriptomic Analysis of Peripheral Blood Reveals a Novel B-Cell Subset in Renal Allograft Recipients With Accommodation.

Frontiers in pharmacology

Zhuang Q,Li H,Peng B,Liu Y,Zhang Y,Cai H,Liu S,Ming Y

56-0038-42 was used in Flow cytometry/Cell sorting to reveal the effect of commonly used IS in clinical practice on B-cell subsets and related mechanism.

Fri Oct 22 00:00:00 EDT 2021

High-throughput compound screen reveals mTOR inhibitors as potential therapeutics to reduce (auto)antibody production by human plasma cells.

European journal of immunology

Tuijnenburg P,Aan de Kerk DJ,Jansen MH,Morris B,Lieftink C,Beijersbergen RL,van Leeuwen EMM,Kuijpers TW

56-0038 was used in Flow cytometry/Cell sorting to explore novel therapeutic targets in signaling pathways essential for plasmablast formation and/or immunoglobulin production.

Wed Jan 01 00:00:00 EST 2020

Comprehensive analysis of T cell immunodominance and immunoprevalence of SARS-CoV-2 epitopes in COVID-19 cases.

bioRxiv : the preprint server for biology

56-0038-42 was used in Flow Cytometry to report a comprehensive map of epitopes recognized by CD4+ and CD8+ T cell responses across the entire SARS-CoV-2 viral proteome.

Wed Dec 09 00:00:00 EST 2020

Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues.

JCI insight

Lowe MM,Boothby I,Clancy S,Ahn RS,Liao W,Nguyen DN,Schumann K,Marson A,Mahuron KM,Kingsbury GA,Liu Z,Munoz Sandoval P,Rodriguez RS,Pauli ML,Taravati K,Arron ST,Neuhaus IM,Harris HW,Kim EA,Shin US,Krummel MF,Daud A,Scharschmidt TC,Rosenblum MD

56-0038 was used in Flow cytometry/Cell sorting to compare healthy skin Tregs with those in peripheral blood, inflamed psoriatic skin, and metastatic melanoma.

Thu Dec 19 00:00:00 EST 2019

CXCR3⁺ T Follicular Helper Cells Induced by Co-Administration of RTS,S/AS01B and Viral-Vectored Vaccines Are Associated With Reduced Immunogenicity and Efficacy Against Malaria.

Frontiers in immunology

Bowyer G,Grobbelaar A,Rampling T,Venkatraman N,Morelle D,Ballou RW,Hill AVS,Ewer KJ

56-0038 was used in Flow cytometry/Cell sorting to illustrate that while a multistage-targeting vaccine strategy could provide high-level efficacy, the regimen design will require careful optimization.

Mon Jan 31 00:00:00 EST 2022

Activation-induced Markers Detect Vaccine-Specific CD4⁺ T Cell Responses Not Measured by Assays Conventionally Used in Clinical Trials.

Vaccines

Bowyer G,Rampling T,Powlson J,Morter R,Wright D,Hill AVS,Ewer KJ

Tue Jul 31 00:00:00 EDT 2018

Safety and Immunogenicity of Malaria Vectored Vaccines Given with Routine Expanded Program on Immunization Vaccines in Gambian Infants and Neonates: A Randomized Controlled Trial.

Frontiers in immunology

Mensah VA,Roetynck S,Kanteh EK,Bowyer G,Ndaw A,Oko F,Bliss CM,Jagne YJ,Cortese R,Nicosia A,Roberts R,D'Alessio F,Leroy O,Faye B,Kampmann B,Cisse B,Bojang K,Gerry S,Viebig NK,Lawrie AM,Clarke E,Imoukhuede EB,Ewer KJ,Hill AVS,Afolabi MO

Mon Mar 14 00:00:00 EDT 2022

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

56-0038 was used in Flow cytometry/Cell sorting to reveal that TIGIT+CD4+T cells provide a supportive microenvironment for CLL cells.

Sat Jan 29 00:00:00 EST 2022

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

56-0038 was used in Flow cytometry/Cell sorting to investigate the use of histone deacetylase inhibitors with immune checkpoint inhibitors in triple negative breast cancer.

Tue Dec 26 00:00:00 EST 2017

T-cell activation is an immune correlate of risk in BCG vaccinated infants.

Nature communications

Fletcher HA,Snowden MA,Landry B,Rida W,Satti I,Harris SA,Matsumiya M,Tanner R,O'Shea MK,Dheenadhayalan V,Bogardus L,Stockdale L,Marsay L,Chomka A,Harrington-Kandt R,Manjaly-Thomas ZR,Naranbhai V,Stylianou E,Darboe F,Penn-Nicholson A,Nemes E,Hatherill M,Hussey G,Mahomed H,Tameris M,McClain JB,Evans TG,Hanekom WA,Scriba TJ,McShane H

56-0038 was used in Flow cytometry/Cell sorting to identify immune characteristics that correlate with an increased risk of tuberculosis contraction using trial and study data.

Tue Apr 12 00:00:00 EDT 2016

PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer.

Cancer immunology research

Severson JJ,Serracino HS,Mateescu V,Raeburn CD,McIntyre RC,Sams SB,Haugen BR,French JD

56-0038 was used in Flow cytometry/Cell sorting to determine that the release of PD-1 and Tim-3, and the inhibition of Tregs, may encourage tumour elimination in metastatic differentiated thyroid cancer.

Mon Jun 01 00:00:00 EDT 2015

Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus.

Journal of immunology (Baltimore, Md. : 1950)

Tsai CY,Liong KH,Gunalan MG,Li N,Lim DS,Fisher DA,MacAry PA,Leo YS,Wong SC,Puan KJ,Wong SB

56-0038 was used in Flow cytometry/Cell sorting to investigate the markers of cellular innate immunity against dengue virus, specifically how type I IFNs and IL-18 regulate activation, proliferation and degranulation of T cells against dendritic cells in human patients with dengue virus.

Wed Apr 15 00:00:00 EDT 2015

Ratiometric analysis of fura red by flow cytometry: a technique for monitoring intracellular calcium flux in primary cell subsets.

PloS one

Wendt ER,Ferry H,Greaves DR,Keshav S

56-0038 was used in Flow cytometry/Cell sorting to describe a technique for monitoring calcium flux in both discrete and heterogeneous populations of primary cells, using a single calcium dye.

Tue Mar 15 00:00:00 EDT 2016

Highly heterogeneous, activated, and short-lived regulatory T cells during chronic filarial infection.

European journal of immunology

Metenou S,Coulibaly YI,Sturdevant D,Dolo H,Diallo AA,Soumaoro L,Coulibaly ME,Kanakabandi K,Porcella SF,Klion AD,Nutman TB

56-0038 was used in Flow cytometry/Cell sorting to study how in filarial infection, the expanded Treg-cell populations are heterogeneous, short-lived, activated, and express higher levels of molecules known to modulate immune responsiveness.

Tue Jul 01 00:00:00 EDT 2014

Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.

Journal of immunology (Baltimore, Md. : 1950)

Arlehamn CL,Seumois G,Gerasimova A,Huang C,Fu Z,Yue X,Sette A,Vijayanand P,Peters B

56-0038 was used in Flow cytometry/Cell sorting to study the role of CD4(+) T cells in latent tuberculosis infection, showing that tuberculosis antigen-specific T cells have novel multifunctional features.

Mon Sep 15 00:00:00 EDT 2014

CD16+ monocytes control T-cell subset development in immune thrombocytopenia.

Blood

Zhong H,Bao W,Li X,Miller A,Seery C,Haq N,Bussel J,Yazdanbakhsh K

56-0038 was used in Flow cytometry/Cell sorting to examine the contribution of monocyte subsets in the control of both regulatory and helper T cell development in patients with immune thrombocytopenia.

Thu Oct 18 00:00:00 EDT 2012

Programmed death-1+ T cells and regulatory T cells are enriched in tumor-involved lymph nodes and associated with aggressive features in papillary thyroid cancer.

The Journal of clinical endocrinology and metabolism

French JD,Kotnis GR,Said S,Raeburn CD,McIntyre RC,Klopper JP,Haugen BR

56-0038 was used in Flow cytometry/Cell sorting to characterise the CD4(+) T cell response to metastatic PTC.

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

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CD3 Monoclonal Antibody (UCHT1), Alexa Fluor™ 700, eBioscience™

CD3 Antibody (56-0038-42) in Flow

Product Details

56-0038-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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