Invitrogen

CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE, eBioscience™

10 Published Figures

19 References

View all (88) LAMP1 (CD107a) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (10)

CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 11

CD107a (LAMP-1) Antibody (12-1079-42) in Flow

Intracellular staining of Jurkat cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD107a (LAMP-1) PE (purple histogram). Total cells were used for analysis.

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Product Details

12-1079-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioH4A3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10853326

Product Specific Information

Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells.

Applications Reported: This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay.

Applications Tested: This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer’s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Soluble NKG2D ligands impair CD8⁺ T cell antitumor function dependent of NKG2D downregulation in neuroblastoma.

Oncology letters

Zhang Y,Luo F,Dong K

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 12-1079-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Expansion of KRAS hotspot mutations reactive T cells from human pancreatic tumors using autologous T cells as the antigen-presenting cells.

Cancer immunology, immunotherapy : CII

Wang S,Zhang X,Zou X,Wen M,Gan C,Jiang X,Li M,Shen R,Zhu D,Yao A,Fang Y,Fox BA,Hu HM,Yu G,Wang X

12-1079-42 was used in Flow cytometry/Cell sorting to study adoptive cell therapy (ACT) with expanded tumor-infiltrating lymphocytes (TIL) or TCR gene-modified T cells (TCR-T) that recognize mutant KRAS neo-antigens can mediate tumor regression in patients with advanced pancreatic ductal adenocarcinoma.

Mon May 01 00:00:00 EDT 2023

Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab.

Signal transduction and targeted therapy

Zheng G,Guo Z,Li W,Xi W,Zuo B,Zhang R,Wen W,Yang AG,Jia L

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 12-1079-42) in Flow Cytometry

Wed Jun 23 00:00:00 EDT 2021

Circulating prostate cancer cells have differential resistance to fluid shear stress-induced cell death.

Journal of cell science

Hope JM,Bersi MR,Dombroski JA,Clinch AB,Pereles RS,Merryman WD,King MR

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 12-1079-42) in Flow Cytometry

Mon Feb 22 00:00:00 EST 2021

HLA-B*27:05 alters immunodominance hierarchy of universal influenza-specific CD8+ T cells.

PLoS pathogens

Sant S,Quiñones-Parra SM,Koutsakos M,Grant EJ,Loudovaris T,Mannering SI,Crowe J,van de Sandt CE,Rimmelzwaan GF,Rossjohn J,Gras S,Loh L,Nguyen THO,Kedzierska K

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 12-1079-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

MAVS Genetic Variation Is Associated with Decreased HIV-1 Replication In Vitro and Reduced CD4⁺ T Cell Infection in HIV-1-Infected Individuals.

Viruses

Stunnenberg M,van Pul L,Sprokholt JK,van Dort KA,Gringhuis SI,Geijtenbeek TBH,Kootstra NA

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 12-1079-42) in Flow Cytometry

Thu Jul 16 00:00:00 EDT 2020

PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytes.

Science advances

Menasche BL,Davis EM,Wang S,Ouyang Y,Li S,Yu H,Shen J

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 12-1079-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Co-immunization of DNA and Protein in the Same Anatomical Sites Induces Superior Protective Immune Responses against SHIV Challenge.

Cell reports

Felber BK,Lu Z,Hu X,Valentin A,Rosati M,Remmel CAL,Weiner JA,Carpenter MC,Faircloth K,Stanfield-Oakley S,Williams WB,Shen X,Tomaras GD,LaBranche CC,Montefiori D,Trinh HV,Rao M,Alam MS,Vandergrift NA,Saunders KO,Wang Y,Rountree W,Das J,Alter G,Reed SG,Aye PP,Schiro F,Pahar B,Dufour JP,Veazey RS,Marx PA,Venzon DJ,Shaw GM,Ferrari G,Ackerman ME,Haynes BF,Pavlakis GN

12-1079 was used in Flow cytometry/Cell sorting to compare immunogenicity and protective efficacy of an HIV vaccine comprised of env and gag DNA and Env (Envelope) proteins by co-administration of the vaccine components in the same muscles or by separate administration of DNA + protein in contralateral sites.

Tue May 12 00:00:00 EDT 2020

Immunoadjuvant Activity of Fucoidans from the Brown Alga Fucus evanescens.

Marine drugs

Kuznetsova TA,Smolina TP,Makarenkova ID,Ivanushko LA,Persiyanova EV,Ermakova SP,Silchenko AS,Zaporozhets TS,Besednova NN,Fedyanina LN,Kryzhanovsky SP

12-1079 was used in Flow cytometry/Cell sorting to comparatively evaluate the effect of fucoidans from the brown alga Fucus evanescens on effector functions of innate and adaptive immunity cells in vitro and in vivo.

Wed Mar 11 00:00:00 EDT 2020

PD1 is expressed on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients.

Oncotarget

Sponaas AM,Yang R,Rustad EH,Standal T,Thoresen AS,Dao Vo C,Waage A,Slørdahl TS,Børset M,Sundan A

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 12-1079-42) in Flow Cytometry

Fri Aug 10 00:00:00 EDT 2018

Complement receptor CD46 co-stimulates optimal human CD8⁺ T cell effector function via fatty acid metabolism.

Nature communications

Arbore G,West EE,Rahman J,Le Friec G,Niyonzima N,Pirooznia M,Tunc I,Pavlidis P,Powell N,Li Y,Liu P,Servais A,Couzi L,Fremeaux-Bacchi V,Placais L,Ferraro A,Walsh PR,Kavanagh D,Afzali B,Lavender P,Lachmann HJ,Kemper C

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 12-1079-42) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

The effect of propofol and sevoflurane on cancer cell, natural killer cell, and cytotoxic T lymphocyte function in patients undergoing breast cancer surgery: an in vitro analysis.

BMC cancer

Lim JA,Oh CS,Yoon TG,Lee JY,Lee SH,Yoo YB,Yang JH,Kim SH

12-1079 was used in Flow cytometry/Cell sorting to identify that propofol was not superior to sevoflurane during anaesthesia for breast cancer surgery in a clinical environment.

Wed Feb 07 00:00:00 EST 2018

Self-reactive IgE exacerbates interferon responses associated with autoimmunity.

Nature immunology

Henault J,Riggs JM,Karnell JL,Liarski VM,Li J,Shirinian L,Xu L,Casey KA,Smith MA,Khatry DB,Izhak L,Clarke L,Herbst R,Ettinger R,Petri M,Clark MR,Mustelin T,Kolbeck R,Sanjuan MA

12-1079 was used in Immunofluorescence to investigate the role of IgE in systemic lupus erythematosus.

Mon Feb 01 00:00:00 EST 2016

T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.

Oncoimmunology

Singh N,Kulikovskaya I,Barrett DM,Binder-Scholl G,Jakobsen B,Martinez D,Pawel B,June CH,Kalos MD,Grupp SA

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 12-1079-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

The human CD8+ T cell responses induced by a live attenuated tetravalent dengue vaccine are directed against highly conserved epitopes.

Journal of virology

Weiskopf D,Angelo MA,Bangs DJ,Sidney J,Paul S,Peters B,de Silva AD,Lindow JC,Diehl SA,Whitehead S,Durbin A,Kirkpatrick B,Sette A

12-1079 was used in Flow cytometry/Cell sorting to characterise the CD8(+) T cell responses after live attenuated dengue vaccination.

Thu Jan 01 00:00:00 EST 2015

Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes.

Traffic (Copenhagen, Denmark)

Dieckmann NM,Hackmann Y,Aricò M,Griffiths GM

12-1079 was used in Flow cytometry/Cell sorting to support a role for Munc18-2 in chaperoning STX11 to the plasma membrane where the final fusion events involved in secretion occur.

Tue Dec 01 00:00:00 EST 2015

Type I IFNs and IL-18 regulate the antiviral response of primary human γδ T cells against dendritic cells infected with Dengue virus.

Journal of immunology (Baltimore, Md. : 1950)

Tsai CY,Liong KH,Gunalan MG,Li N,Lim DS,Fisher DA,MacAry PA,Leo YS,Wong SC,Puan KJ,Wong SB

12-1079 was used in Flow cytometry/Cell sorting to investigate the markers of cellular innate immunity against dengue virus, specifically how type I IFNs and IL-18 regulate activation, proliferation and degranulation of T cells against dendritic cells in human patients with dengue virus.

Wed Apr 15 00:00:00 EDT 2015

The secretion of IL-22 from mucosal NKp44⁺ NK cells is associated with microbial translocation and virus infection in SIV/SHIV-infected Chinese macaques.

Journal of immunology research

Wang W,Wu F,Cong Z,Liu K,Qin C,Wei Q

12-1079 was used in Flow cytometry/Cell sorting to investigate the role of a novel subtype of innate lymphoid cells, the NKp44(+) NK cells, in SIV-induced microbial translocation.

Tue Dec 01 00:00:00 EST 2015

An efficient strategy to induce and maintain in vitro human T cells specific for autologous non-small cell lung carcinoma.

PloS one

Canderan G,Gruarin P,Montagna D,Fontana R,Campi G,Melloni G,Traversari C,Dellabona P,Casorati G

12-1079 was used in Flow cytometry/Cell sorting to efficiently induce and propagate in vitro T cells specific for non-small cell lung carcinoma, starting from autologous peripheral blood lymphocytes.

Mon Aug 09 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD107 antigen-like family member A; CD107a; LAMP-1; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; lysosome-associated membrane protein 1

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Gene Aliases: CD107a; LAMP1; LAMPA; LGP120

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UniProt ID: (Human) P11279

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Entrez Gene ID: (Human) 3916

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Function(s)

virus receptor activity protein binding enzyme binding protein domain specific binding membrane trafficking regulatory protein

Process(es)

autophagy granzyme-mediated apoptotic signaling pathway positive regulation of natural killer cell degranulation positive regulation of natural killer cell mediated cytotoxicity viral entry into host cell autophagic cell death protein stabilization establishment of protein localization to organelle Golgi to lysosome transport regulation of organelle transport along microtubule

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE, eBioscience™

CD107a (LAMP-1) Antibody (12-1079-42) in Flow

Product Details

12-1079-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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