Invitrogen

CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE-Cyanine5, eBioscience™

10 Published Figures

20 References

View all (88) LAMP1 (CD107a) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (10)

CD107a (LAMP-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 11

CD107a (LAMP-1) Antibody (15-1079-42) in Flow

Intracellular staining of Jurkat cell line with 0.25 µg of Mouse IgG1 K Isotype Control PE-Cyanine5 (Product # 15-4714-81) (open histogram) or Anti-Human CD107a (LAMP-1) PE-Cyanine5 (filled histogram).Total cells were used for analysis.

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Product Details

15-1079-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 18 publications 18 publications

Functional Assay (Functional)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioH4A3

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10547280

Product Specific Information

Description: The eBioH4A3 monoclonal antibody reacts with human CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a highly glycosylated protein of approximately 110kDa. It is predominantly expressed intracellularly in the lysosomal/endosomal membrane in nearly all cells. CD107a is transiently expressed on the cell surface of degranulating cytolytic T cells, and is also upregulated on the surface of activated platelets and some cancer cells.

Applications Reported: This eBioH4A3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay.

Applications Tested: This eBioH4A3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer’s Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Soluble NKG2D ligands impair CD8⁺ T cell antitumor function dependent of NKG2D downregulation in neuroblastoma.

Oncology letters

Zhang Y,Luo F,Dong K

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 15-1079-42) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab.

Signal transduction and targeted therapy

Zheng G,Guo Z,Li W,Xi W,Zuo B,Zhang R,Wen W,Yang AG,Jia L

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 15-1079-42) in Flow Cytometry

Wed Jun 23 00:00:00 EDT 2021

Circulating prostate cancer cells have differential resistance to fluid shear stress-induced cell death.

Journal of cell science

Hope JM,Bersi MR,Dombroski JA,Clinch AB,Pereles RS,Merryman WD,King MR

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 15-1079-42) in Flow Cytometry

Mon Feb 22 00:00:00 EST 2021

Effects of therapeutic vaccination on the control of SIV in rhesus macaques with variable responsiveness to antiretroviral drugs.

PloS one

Tunggal HC,Munson PV,O'Connor MA,Hajari N,Dross SE,Bratt D,Fuller JT,Bagley K,Fuller DH

15-1079-42 was used in Flow Cytometry to indicate that mucosal immune responses, present prior to infection, can influence efficacy of antiretroviral therapy and the outcome of immunotherapeutic vaccination, suggesting that therapies capable of modulating host mucosal responses may be needed to achieve HIV cure.

Tue Nov 16 00:00:00 EST 2021

HLA-B*27:05 alters immunodominance hierarchy of universal influenza-specific CD8+ T cells.

PLoS pathogens

Sant S,Quiñones-Parra SM,Koutsakos M,Grant EJ,Loudovaris T,Mannering SI,Crowe J,van de Sandt CE,Rimmelzwaan GF,Rossjohn J,Gras S,Loh L,Nguyen THO,Kedzierska K

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 15-1079-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

MAVS Genetic Variation Is Associated with Decreased HIV-1 Replication In Vitro and Reduced CD4⁺ T Cell Infection in HIV-1-Infected Individuals.

Viruses

Stunnenberg M,van Pul L,Sprokholt JK,van Dort KA,Gringhuis SI,Geijtenbeek TBH,Kootstra NA

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 15-1079-42) in Flow Cytometry

Thu Jul 16 00:00:00 EDT 2020

PBRM1 and the glycosylphosphatidylinositol biosynthetic pathway promote tumor killing mediated by MHC-unrestricted cytotoxic lymphocytes.

Science advances

Menasche BL,Davis EM,Wang S,Ouyang Y,Li S,Yu H,Shen J

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 15-1079-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Simian-Human Immunodeficiency Virus SHIV.CH505 Infection of Rhesus Macaques Results in Persistent Viral Replication and Induces Intestinal Immunopathology.

Journal of virology

Bar KJ,Coronado E,Hensley-McBain T,O'Connor MA,Osborn JM,Miller C,Gott TM,Wangari S,Iwayama N,Ahrens CY,Smedley J,Moats C,Lynch RM,Haddad EK,Haigwood NL,Fuller DH,Shaw GM,Klatt NR,Manuzak JA

15-1079-42 was used in Flow cytometry/Cell sorting to evaluate the viral dynamics and immunopathology of SHIV.CH505 in rhesus macaques.

Sun Sep 15 00:00:00 EDT 2019

Differential Effect of Mucosal NKp44⁺ Innate Lymphoid Cells and Δγ Cells on Simian Immunodeficiency Virus Infection Outcome in Rhesus Macaques.

Journal of immunology (Baltimore, Md. : 1950)

Rahman MA,Ko EJ,Enyindah-Asonye G,Helmold Hait S,Hogge C,Hunegnaw R,Venzon DJ,Hoang T,Robert-Guroff M

15-1079-42 was used in Flow cytometry/Cell sorting to investigate NK cell and innate lymphoid cell (ILC) dynamics and function in rhesus macaque rectal tissue and blood following mucosal priming with replicating adenovirus (Ad)-SIV recombinants, systemic boosting with SIV envelope protein, and subsequent repeated low-dose intravaginal SIV exposures.

Fri Nov 01 00:00:00 EDT 2019

Characterization of Antigenic MHC-Class-I-Restricted T Cell Epitopes in the Glycoprotein of Ebolavirus.

Cell reports

Powlson J,Wright D,Zeltina A,Giza M,Nielsen M,Rampling T,Venkatrakaman N,Bowden TA,Hill AVS,Ewer KJ

15-1079 was used in Flow cytometry/Cell sorting to characterize nine immunogenic epitopes on the Ebolavirus GP.

Tue Nov 26 00:00:00 EST 2019

PD1 is expressed on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients.

Oncotarget

Sponaas AM,Yang R,Rustad EH,Standal T,Thoresen AS,Dao Vo C,Waage A,Slørdahl TS,Børset M,Sundan A

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 15-1079-42) in Flow Cytometry

Fri Aug 10 00:00:00 EDT 2018

Complement receptor CD46 co-stimulates optimal human CD8⁺ T cell effector function via fatty acid metabolism.

Nature communications

Arbore G,West EE,Rahman J,Le Friec G,Niyonzima N,Pirooznia M,Tunc I,Pavlidis P,Powell N,Li Y,Liu P,Servais A,Couzi L,Fremeaux-Bacchi V,Placais L,Ferraro A,Walsh PR,Kavanagh D,Afzali B,Lavender P,Lachmann HJ,Kemper C

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 15-1079-42) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

Activation-induced Markers Detect Vaccine-Specific CD4⁺ T Cell Responses Not Measured by Assays Conventionally Used in Clinical Trials.

Vaccines

Bowyer G,Rampling T,Powlson J,Morter R,Wright D,Hill AVS,Ewer KJ

15-1079 was used in Functional assays to compare the use of markers for their sensitivity, specificity and associations with other measures of vaccine immunogenicity and demonstrate these as an additional method of reporting the T cell response to vaccination.

Tue Jul 31 00:00:00 EDT 2018

Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection.

PLoS pathogens

Demers KR,Makedonas G,Buggert M,Eller MA,Ratcliffe SJ,Goonetilleke N,Li CK,Eller LA,Rono K,Maganga L,Nitayaphan S,Kibuuka H,Routy JP,Slifka MK,Haynes BF,McMichael AJ,Bernard NF,Robb ML,Betts MR

15-1079 was used in Flow cytometry/Cell sorting to assess the CD8+ T cell functional evolution from primary to chronic HIV infection, demonstrating that progressive transcriptional dysregulation leads to the reduced CD8+ T cell perforin expression and loss of cytolytic potential characteristic of chronic HIV infection.

Mon Aug 01 00:00:00 EDT 2016

T cells targeting NY-ESO-1 demonstrate efficacy against disseminated neuroblastoma.

Oncoimmunology

Singh N,Kulikovskaya I,Barrett DM,Binder-Scholl G,Jakobsen B,Martinez D,Pawel B,June CH,Kalos MD,Grupp SA

Published figure using CD107a (LAMP-1) monoclonal antibody (Product # 15-1079-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo.

Scientific reports

Kritikou JS,Dahlberg CI,Baptista MA,Wagner AK,Banerjee PP,Gwalani LA,Poli C,Panda SK,Kärre K,Kaech SM,Wermeling F,Andersson J,Orange JS,Brauner H,Westerberg LS

15-1079 was used in Flow cytometry/Cell sorting to study loss of function WASp mutations and natural killer cell function in patients with Wiskott-Aldrich syndrome.

Mon Aug 01 00:00:00 EDT 2016

Dual-Affinity Re-Targeting proteins direct T cell-mediated cytolysis of latently HIV-infected cells.

The Journal of clinical investigation

Sung JA,Pickeral J,Liu L,Stanfield-Oakley SA,Lam CY,Garrido C,Pollara J,LaBranche C,Bonsignori M,Moody MA,Yang Y,Parks R,Archin N,Allard B,Kirchherr J,Kuruc JD,Gay CL,Cohen MS,Ochsenbauer C,Soderberg K,Liao HX,Montefiori D,Moore P,Johnson S,Koenig S,Haynes BF,Nordstrom JL,Margolis DM,Ferrari G

15-1079 was used in Flow cytometry/Cell sorting to develop an immunotherapeutic modality aimed to improve T cell-mediated clearance of HIV-1-infected cells.

Mon Nov 02 00:00:00 EST 2015

Initial HIV-1 antigen-specific CD8+ T cells in acute HIV-1 infection inhibit transmitted/founder virus replication.

Journal of virology

Freel SA,Picking RA,Ferrari G,Ding H,Ochsenbauer C,Kappes JC,Kirchherr JL,Soderberg KA,Weinhold KJ,Cunningham CK,Denny TN,Crump JA,Cohen MS,McMichael AJ,Haynes BF,Tomaras GD

15-1079-42 was used in Functional assays to examine the timing and HIV-1 antigen specificity of antiviral CD8(+) T cells during acute HIV-1 infection.

Fri Jun 01 00:00:00 EDT 2012

Phosphorylation of the myosin IIA tailpiece regulates single myosin IIA molecule association with lytic granules to promote NK-cell cytotoxicity.

Blood

Sanborn KB,Mace EM,Rak GD,Difeo A,Martignetti JA,Pecci A,Bussel JB,Favier R,Orange JS

15-1079 was used in Flow cytometry/Cell sorting to evaluate the nature of the association of myosin IIA with lytic granules in the formation of the immunological synapse in natural killer-cell cytotoxicity.

Thu Nov 24 00:00:00 EST 2011

Phagocytosis of dying tumor cells by human peritoneal mesothelial cells.

Journal of cell science

Wagner BJ,Lindau D,Ripper D,Stierhof YD,Glatzle J,Witte M,Beck H,Keppeler H,Lauber K,Rammensee HG,Königsrainer A

15-1079 was used in Flow cytometry/Cell sorting to identify a novel, 'amateur' phagocyte responsible for clearing dying tumour cells in the peritoneum.

Sun May 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD107 antigen-like family member A; CD107a; LAMP-1; lysosomal-associated membrane protein 1; Lysosome-associated membrane glycoprotein 1; lysosome-associated membrane protein 1

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Gene Aliases: CD107a; LAMP1; LAMPA; LGP120

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UniProt ID: (Human) P11279

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Entrez Gene ID: (Human) 3916

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Function(s)

virus receptor activity protein binding enzyme binding protein domain specific binding membrane trafficking regulatory protein

Process(es)

autophagy granzyme-mediated apoptotic signaling pathway positive regulation of natural killer cell degranulation positive regulation of natural killer cell mediated cytotoxicity viral entry into host cell autophagic cell death protein stabilization establishment of protein localization to organelle Golgi to lysosome transport regulation of organelle transport along microtubule

Performance Guarantee

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CD107a (LAMP-1) Monoclonal Antibody (eBioH4A3), PE-Cyanine5, eBioscience™

CD107a (LAMP-1) Antibody (15-1079-42) in Flow

Product Details

15-1079-42

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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