Invitrogen

CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), PE, eBioscience™

CD223 (LAG-3) Antibody in Flow Cytometry (Flow)

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CD223 (LAG-3) Antibody (12-2231-82) in Flow

Staining of 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated C57Bl/6 splenocytes with 0.25 µg of Rat IgG1 K Isotype Control PE (Product # 12-4301-82) (blue histogram) or 0.25 µg of Anti-Mouse CD223 (Lag-3) PE (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450, were used for analysis.

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Product Details

12-2231-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioC9B7W (C9B7W)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_494214

Product Specific Information

Description: The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells. CD223 is a 70 kDa type I transmembrane protein with a structure that is similar to CD4. However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain. This results in a 54 kDa fragment containing all the extracellular domains, and a 16 kDa fragment containing the intracellular and transmembrane domains. The 54 kDa can remain membrane-associated or be released as soluble CD223.

CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation. Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function.

Applications Reported: This eBioC9B7W (C9B7W) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioC9B7W (C9B7W) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

BRCA1 deficiency in mature CD8⁺ T lymphocytes impairs antitumor immunity.

Journal for immunotherapy of cancer

Wu B,Qi L,Chiang HC,Pan H,Zhang X,Greenbaum A,Stark E,Wang LJ,Chen Y,Haddad BR,Clagett D,Isaacs C,Elledge R,Horvath A,Hu Y,Li R

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 12-2231-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 12-2231-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Blocking IL1 Beta Promotes Tumor Regression and Remodeling of the Myeloid Compartment in a Renal Cell Carcinoma Model: Multidimensional Analyses.

Clinical cancer research : an official journal of the American Association for Cancer Research

Aggen DH,Ager CR,Obradovic AZ,Chowdhury N,Ghasemzadeh A,Mao W,Chaimowitz MG,Lopez-Bujanda ZA,Spina CS,Hawley JE,Dallos MC,Zhang C,Wang V,Li H,Guo XV,Drake CG

12-2231 was used in Flow cytometry/Cell sorting to evaluate clinically relevant combinations of anti-IL-1b plus either anti-PD-1 or the multi-targeted tyrosine kinase inhibitor (TKI) cabozantinib.

Fri Jan 15 00:00:00 EST 2021

PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer.

Nature communications

Sun Y,Lu D,Yin Y,Song J,Liu Y,Hao W,Qi F,Zhang G,Zhang X,Liu L,Lin Z,Liang H,Zhao X,Jin Y,Yin Y

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 12-2231-82) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

A Fas-4-1BB fusion protein converts a death to a pro-survival signal and enhances T cell therapy.

The Journal of experimental medicine

Oda SK,Anderson KG,Ravikumar P,Bonson P,Garcia NM,Jenkins CM,Zhuang S,Daman AW,Chiu EY,Bates BM,Greenberg PD

12-2231-82 was used in Flow Cytometry to develop enhanced adoptive T cell therapy using immunomodulatory fusion proteins for use in solid tumours.

Mon Dec 07 00:00:00 EST 2020

Targeting CMTM6 Suppresses Stem Cell-Like Properties and Enhances Antitumor Immunity in Head and Neck Squamous Cell Carcinoma.

Cancer immunology research

Chen L,Yang QC,Li YC,Yang LL,Liu JF,Li H,Xiao Y,Bu LL,Zhang WF,Sun ZJ

12-2231 was used in Flow cytometry/Cell sorting to investigate the correlation of CMTM6 expression, a regulator of PD-L1 expression, with the prognosis of patients with Head and Neck Squamous Cell Carcinoma.

Sat Feb 01 00:00:00 EST 2020

LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma Cells.

Immunity

Lino AC,Dang VD,Lampropoulou V,Welle A,Joedicke J,Pohar J,Simon Q,Thalmensi J,Baures A,Flühler V,Sakwa I,Stervbo U,Ries S,Jouneau L,Boudinot P,Tsubata T,Adachi T,Hutloff A,Dörner T,Zimber-Strobl U,de Vos AF,Dahlke K,Loh G,Korniotis S,Goosmann C,Weill JC,Reynaud CA,Kaufmann SHE,Walter J,Fillatreau S

12-2231 was used in Flow cytometry/Cell sorting to identify a natural plasma cell subset that distinctively expresses the inhibitory receptor LAG-3 and mediates this function in vivo.

Tue Jul 17 00:00:00 EDT 2018

Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.

Nature communications

Liu Z,Ravindranathan R,Kalinski P,Guo ZS,Bartlett DL

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 12-2231-82) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2017

Protein Tyrosine Phosphatase Inhibition Prevents Experimental Cerebral Malaria by Precluding CXCR3 Expression on T Cells.

Scientific reports

Van Den Ham KM,Smith LK,Richer MJ,Olivier M

12-2231 was used in Flow cytometry/Cell sorting to demonstrate that inhibition of protein tyrosine phosphatase activity significantly attenuates T cell sequestration within the brain and prevents the development of neuropathology.

Fri Jul 14 00:00:00 EDT 2017

Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth.

Nature communications

Klein JC,Moses K,Zelinskyy G,Sody S,Buer J,Lang S,Helfrich I,Dittmer U,Kirschning CJ,Brandau S

12-2231 was used in Flow cytometry/Cell sorting to demonstrate that cells deficient in endosomal TLR3, 7 and 9 leads to tumour regression and anti-tumour immunity.

Thu Mar 16 00:00:00 EDT 2017

Epithelia Use Butyrophilin-like Molecules to Shape Organ-Specific γδ T Cell Compartments.

Cell

Di Marco Barros R,Roberts NA,Dart RJ,Vantourout P,Jandke A,Nussbaumer O,Deban L,Cipolat S,Hart R,Iannitto ML,Laing A,Spencer-Dene B,East P,Gibbons D,Irving PM,Pereira P,Steinhoff U,Hayday A

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 12-2231-82) in Flow Cytometry

Thu Sep 22 00:00:00 EDT 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 12-2231-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression.

Oncoimmunology

Chen L,Yi X,Goswami S,Ahn YH,Roybal JD,Yang Y,Diao L,Peng D,Peng D,Fradette JJ,Wang J,Byers LA,Kurie JM,Ullrich SE,Qin FX,Gibbons DL

12-2231 was used in Flow cytometry/Cell sorting to show that bone morphogenetic protein-4 causes immunosuppression by augmenting PD-L1 expression in the mesenchymal subset of lung cancer cells.

Tue Jan 12 00:00:00 EST 2021

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 12-2231-82) in Flow Cytometry

Tue Oct 28 00:00:00 EDT 2014

Sequential transcriptional changes dictate safe and effective antigen-specific immunotherapy.

Nature communications

Burton BR,Britton GJ,Fang H,Verhagen J,Smithers B,Sabatos-Peyton CA,Carney LJ,Gough J,Strobel S,Wraith DC

12-2231 was used in Flow cytometry/Cell sorting to study the markers significant for antigen-specific immunotherapy such as regulatory CD4(+) T-cell transcription factors.

Wed Sep 03 00:00:00 EDT 2014

Regulatory T cells that recognize a ubiquitous stress-inducible self-antigen are long-lived suppressors of autoimmune arthritis.

Proceedings of the National Academy of Sciences of the United States of America

van Herwijnen MJ,Wieten L,van der Zee R,van Kooten PJ,Wagenaar-Hilbers JP,Hoek A,den Braber I,Anderton SM,Singh M,Meiring HD,van Els CA,van Eden W,Broere F

12-2231 was used in Flow cytometry/Cell sorting to uncover potent disease-suppressive Treg cells that recognise Hsp 70, with the potential for re-establishing self-tolerance in autoimmunity.

Tue Aug 28 00:00:00 EDT 2012

Modulation of redox balance leaves murine diabetogenic TH1 T cells "LAG-3-ing" behind.

Diabetes

Delmastro MM,Styche AJ,Trucco MM,Workman CJ,Vignali DA,Piganelli JD

12-2231 was used in Flow cytometry/Cell sorting to investigate how soluble LAG-3 can serve as an early T-cell-specific biomarker for type 1 diabetes onset and immunomodulation.

Sun Jul 01 00:00:00 EDT 2012

TCR-dependent and -independent activation underlie liver-specific regulation of NKT cells.

Journal of immunology (Baltimore, Md. : 1950)

Subleski JJ,Hall VL,Wolfe TB,Scarzello AJ,Weiss JM,Chan T,Hodge DL,Back TC,Ortaldo JR,Wiltrout RH

12-2231 was used in Flow cytometry/Cell sorting to investigate TCR -dependent and -independent activation of NKT cells in the liver.

Sat Jan 15 00:00:00 EST 2011

Effector/memory but not naive regulatory T cells are responsible for the loss of concomitant tumor immunity.

Journal of immunology (Baltimore, Md. : 1950)

Lin YC,Chang LY,Huang CT,Peng HM,Dutta A,Chen TC,Yeh CT,Lin CY

12-2231 was used in Flow cytometry/Cell sorting to study the contribution of various Treg cell types to the maintenance of concomitant tumour immunity.

Fri May 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: Activation-induced cytidine deaminase-linked autoimmunity protein; Aida; CD223; LAG-3; Lymphocyte activation gene 3 protein; sLAG 3; sLAG3; soluble LAG 3lymphocyte activating 3; soluble LAG3

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Gene Aliases: CD223; LAG-3; Lag3; Ly66

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UniProt ID: (Mouse) Q61790

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Entrez Gene ID: (Mouse) 16768

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Function(s)

transmembrane signaling receptor activity MHC class II protein binding transmembrane signal receptor

Process(es)

cell surface receptor signaling pathway negative regulation of interleukin-2 biosynthetic process positive regulation of natural killer cell mediated cytotoxicity negative regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), PE, eBioscience™

CD223 (LAG-3) Antibody (12-2231-82) in Flow

Product Details

12-2231-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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