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Invitrogen
CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), Brilliant Violet™ 480, eBioscience™
FIGURE: 1 / 1
CD223 (LAG-3) Antibody (414-2231-82) in Flow
Swiss Webster mouse splenocytes were unstimulated (left) or stimulated for 72 hours with CD3e and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0031-85) and (Product # 16-0281-85) (right). Cells were then stained with CD8a Monoclonal Antibody, FITC (Product # 11-0081-82) and 1.0 µg of CD223 (LAG-3) Monoclonal Antibody, Brilliant Violet 480. Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
414-2231-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioC9B7W (C9B7W)
Conjugate
Excitation/Emission Max
437/478 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Product Specific Information
Description: The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells. CD223 is a 70 kDa type I transmembrane protein with a structure that is similar to CD4. However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain. This results in a 54 kDa fragment containing all the extracellular domains, and a 16 kDa fragment containing the intracellular and transmembrane domains. The 54 kDa can remain membrane-associated or be released as soluble CD223.
CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation. Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function.
Applications Reported: This eBioC9B7W (C9B7W) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBioC9B7W (C9B7W) antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Brilliant Violet™ 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™
Target Information
LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activation-induced cytidine deaminase-linked autoimmunity protein; Aida; CD223; LAG-3; Lymphocyte activation gene 3 protein; sLAG 3; sLAG3; soluble LAG 3lymphocyte activating 3; soluble LAG3
Gene Aliases: CD223; LAG-3; Lag3; Ly66
UniProt ID: (Mouse) Q61790
Entrez Gene ID: (Mouse) 16768
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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